A5056986063- Influenza Virus Research Studies
- Computational Drug Discovery Methods
- Synthesis and biological activity
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Click Chemistry and Applications
- Viral gastroenteritis research and epidemiology
- Essential Oils and Antimicrobial Activity
- Animal Virus Infections Studies
- Diverse Scientific Research Studies
- Synthesis and Characterization of Heterocyclic Compounds
- X-ray Diffraction in Crystallography
- Pharmacological Effects of Natural Compounds
- Crystallization and Solubility Studies
- Cancer therapeutics and mechanisms
- Curcumin's Biomedical Applications
- Monoclonal and Polyclonal Antibodies Research
- Bioactive Compounds and Antitumor Agents
- Phytochemistry and Biological Activities
- COVID-19 epidemiological studies
- Lipid Membrane Structure and Behavior
- Virology and Viral Diseases
- Plant biochemistry and biosynthesis
National Research Centre
2017-2025
National Water Research Center
2019-2025
Ahram Canadian University
2024-2025
Ain Shams University
2023
(1) Background: Drug repositioning is an unconventional drug discovery approach to explore new therapeutic benefits of existing drugs. Currently, it emerges as a rapid avenue alleviate the COVID-19 pandemic disease. (2) Methods: Herein, we tested antiviral activity anti-microbial and anti-inflammatory Food Administration (FDA)-approved drugs, commonly prescribed relieve respiratory symptoms, against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), viral causative agent pandemic....
Six compounds namely, tanshinone IIA (1), carnosic acid (2), rosmarinic (3), salvianolic B (4), baicalein (5), and glycyrrhetinic (6) were screened for their anti-SARS-CoV-2 activities against both the spike (S) main protease (Mpro) receptors using molecular docking studies. Molecular recommended superior affinities of (4) as common results from previously published computational articles. On other hand, actual tested in vitro plaque reduction assay to calculate IC50 values after measuring...
Until now, there has been no direct evidence of the effectiveness repurposed FDA-approved drugs against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infections. Although curcumin, hesperidin, and quercetin have broad spectra pharmacological properties, their antiviral activities SARS-CoV-2 remain unclear. Our study aimed to assess in vitro compared hydroxychloroquine determine mode action. In Vero E6 cells, these compounds significantly inhibited virus replication, mainly as...
Abstract During the current coronavirus disease 2019 (COVID-19) pandemic, symptoms of depression are commonly documented among both symptomatic and asymptomatic quarantined COVID-19 patients. Despite that many FDA-approved drugs have been showed anti-SARS-CoV-2 activity in vitro remarkable efficacy against clinical trials, no pharmaceutical products yet declared to be fully effective for treating COVID-19. Antidepressants comprise five major drug classes treatment depression, neuralgia,...
In this article, 34 anticoagulant drugs were screened in silico against the main protease (Mpro) of SARS-CoV-2 using molecular docking tools. Idraparinux, fondaparinux, eptifibatide, heparin, and ticagrelor demonstrated highest binding affinities towards Mpro. A dynamics study at 200 ns was also carried out for most promising anticoagulants to provide insights into dynamic thermodynamic properties compounds. Moreover, a quantum mechanical conducted which helped us attest some findings....
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe human infections and dromedary camels are considered an intermediary host. The dynamics of natural infection in not well understood. Through systematic surveillance Egypt, nasal, rectal, milk, urine serum samples were collected from between June 2014 February 2016. Locations included quarantines, markets, abattoirs, free-roaming herds farmed breeding herds. overall seroprevalence was 71% RNA detection rate 15%. Imported...
Through surveillance, we isolated and characterized an influenza A virus from Egyptian fruit bats. This had affinity to avian-like receptors but was also able infect mice. Our findings indicate that bats may harbor a diversity of viruses. Such viruses have the potential cross species barrier other species, including domestic birds, mammals, and, possibly, humans.
This report announces the complete genome sequences of two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolates detected in Egypt. The were obtained from oropharyngeal swab specimens Egyptians Upper and Lower Sequence analysis showed mutations that differentiate Egyptian strains reference strain 2019-nCoV WHU01.
Dromedary camels are natural host of the Middle East respiratory syndrome coronavirus (MERS-CoV). However, there limited studies MERS-CoV infection other domestic mammals exposed to infected dromedaries. We expanded our surveillance among in Egypt, Tunisia, and Senegal include mammalian species contact with camels. A total 820 sera 823 nasal swabs from cattle, sheep, goats, donkeys, buffaloes, mules, horses were collected. Swabs tested using RT-PCR virus RNA-positive samples genetically...
In late December 2019, a novel coronavirus, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), escaped the animal–human interface and emerged as an ongoing global pandemic with flu-like illness, commonly known disease 2019 (COVID-19). this study, molecular docking study was carried out for seventeen (17) structural analogues prepared from natural maslinic oleanolic acids, screened against SARS-CoV-2 main protease. Furthermore, we experimentally validated virtual data by...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the latest arisen contagious pathogen related to global outbreak of atypical pneumonia pandemic (COVID-19). The essential oils (EOs) Eucalyptus camaldulensis, E. ficifolia F. Muell., citriodora Hook, globulus Labill, sideroxylon Cunn. ex Woolls, and torquata Luehm. were investigated for its antiviral activity against SARS-CoV-2. EOs phytochemical composition was determined using GC/MS analysis. Correlation with explored also...
Due to the challenges for developing vaccines in devastating pandemic situations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and screening novel antiviral agents are peremptorily demanded. Herein, we developed EGYVIR as a potent immunomodulatory herbal extract with promising activity against SARS-CoV-2. It constitutes combination black pepper curcumin extract. The effect is attributed two key phases disease cases. First, inhibition nuclear translocation NF-kβ p50,...
Dromedary camels are the natural reservoirs of Middle East respiratory syndrome coronavirus (MERS-CoV). Camels mostly bred in African countries then exported into Africa and for consumption. To understand distribution MERS-CoV among North East, we conducted surveillance Egypt, Senegal, Tunisia, Uganda, Jordan, Saudi Arabia, Iraq. We also performed longitudinal studies three camel herds Egypt Jordan to elucidate infection transmission. Between 2016 2018, a total 4027 nasal swabs 3267 serum...
Since the emergence of SARS-CoV-2 at end 2019, 64 candidate vaccines are in clinical development and 173 pre-clinical phase. Five types currently approved for emergency use many countries (Inactivated, Sinopharm; Viral-vector, Astrazeneca, Gamaleya Research Institute; mRNA, Moderna, BioNTech/Pfizer). The main challenge this pandemic was availability to produce an effective vaccine be distributed world’s population a short time. Herein, we developed whole virus NRC-VACC-01 inactivated tested...
Since the emergence of SARS-CoV-2 pandemic in 2019, it has remained a significant global threat, especially with newly evolved variants. Despite presence different COVID-19 vaccines, discovery proper antiviral therapeutics is an urgent necessity. Nature considered as historical trove for drug discovery, crises. During our efforts to discover potential anti-SARS CoV-2 natural therapeutics, screening in-house products and plant crude extracts library led identification C. benedictus extract...
The n -hexane extract showed a significant antiviral activity. In addition, annotated compounds exhibited high binding scores on 6 target viral proteins in silico .
In this article, emulsomes (EMLs) were fabricated to encapsulate the N-(5-nitrothiazol-2-yl)-carboxamido derivatives (3a-3g) in an attempt improve their biological availability and antiviral activity. Next, both cytotoxicity anti-SARS-CoV-2 activities of examined compounds loaded EMLs (F3a-g) assessed Vero E6 cells via MTT assay calculate CC50 inhibitory concentration 50 (IC50) values. The most potent 3e-loaded (F3e) elicited a selectivity index 18 with IC50 value 0.73 μg/mL. Moreover, F3e...
We detected highly pathogenic avian influenza A(H5N1) virus in wild rats collected from a rural area Giza, Egypt, near poultry farms, markets, and backyard flocks. Sequence phylogenetic analyses indicated that the belonged to clade 2.3.4.4b, which has been predominant genotype circulating Egypt worldwide since 2021-2022. Active surveillance of viruses domestic mammals is recommended prevent further spread humans.
Egypt is a hotspot for avian influenza virus (AIV) due to the endemicity of H5N1 and H9N2 viruses. AIVs were isolated from 329 samples collected in 2016–2018; 48% H9N2, 37.1% H5N8, 7.6% H5N1, 7.3% co-infections with 2 3 subtypes. The 32 hemagglutinin (HA) sequences viruses formed well-defined lineage within clade 2.2.1.2. 10 HA H5N8 belonged subclade 2.3.4.4. 11 isolates showed high sequence homology other Egyptian G1-like prevalence ducks (2.4%) was higher than chickens (0.94%). Genetic...
There is an urgent need to develop and synthesize new anti-influenza drugs with activity against different strains, resistance mutations, suitability for various populations. Herein, we tested in vitro vivo the antiviral of 1,2,3-triazole glycosides incorporating benzimidazole, benzooxazole, or benzotriazole cores synthesized by using a click approach. The Cu-catalyzation strategy consisted 1,3-dipolar cycloaddition azidoalkyl derivative respective heterocyclic glycosyl acetylenes five six...