A5027372459- HIV Research and Treatment
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- RNA and protein synthesis mechanisms
- Biosimilars and Bioanalytical Methods
- HIV/AIDS drug development and treatment
- Chromosomal and Genetic Variations
- Cancer Research and Treatments
- HIV-related health complications and treatments
- Bacteriophages and microbial interactions
- Sirtuins and Resveratrol in Medicine
- Bone Tumor Diagnosis and Treatments
- Immune cells in cancer
- Calcium signaling and nucleotide metabolism
- Ginseng Biological Effects and Applications
- Advanced Fluorescence Microscopy Techniques
- Viral Infections and Vectors
- Pharmacological Effects of Natural Compounds
- S100 Proteins and Annexins
- interferon and immune responses
University of California, Los Angeles
2017-2025
Xi'an Jiaotong University
2024
Broad Center
2021-2024
Black AIDS Institute
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2010-2016
Hebei Medical University
2009
Northwestern University
2009
Adoptive cell therapy (ACT) using T cells expressing chimeric antigen receptors (CARs) is an area of intense investigation in the treatment malignancies and chronic viral infections. One limitations ACT-based CAR lack vivo persistence maintenance optimal function. Therefore, alternative strategies that increase function CAR-expressing are needed. In our studies humanized bone marrow/liver/thymus (BLT) mouse model nonhuman primate (NHP) HIV infection, we evaluated two CAR-based gene...
Chimeric Antigen Receptor (CAR) T cell therapy shows promise for various diseases. Our studies in humanized mice and non-human primates (NHPs) demonstrate that hematopoietic stem (HSCs) modified with anti-HIV CAR achieve lifelong engraftment, providing functional anti-viral CAR-T cells reduce viral rebound after ART withdrawal. However, exhaustion due to chronic immune activation remains a key obstacle sustained efficacy, necessitating additional measures cure. We recently showed low dose...
Abstract Mouse somatic cells can be reprogrammed into induced pluripotent stem by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. Together with Sox2 plays a major role as master endogenous genes trigger in reprogramming. It has been reported that Sirtuin 1 (Sirt1), member of the family NAD+-dependent protein deacetylases, is involved embryonic cell antioxidation, differentiation, individual development. However, deacetylation enzyme, whether Sirt1...
Due to the durability and persistence of reservoirs HIV-1-infected cells, combination antiretroviral therapy (ART) is insufficient in eradicating infection. Achieving HIV-1 cure or sustained remission without ART treatment will require enhanced persistent effective antiviral immune responses. Chimeric Antigen Receptor (CAR) T-cells have emerged as a powerful immunotherapy show promise treating Persistence, trafficking, maintenance function remain be challenge many these approaches, which are...
A hallmark of HIV-1 infection is chronic inflammation, even in patients treated with antiretroviral therapy (ART). Chronic inflammation drives pathogenesis, leading to loss CD4+ T cells and exhaustion antiviral immunity. Therefore, strategies safely reduce systematic are needed halt disease progression restore defective immune responses. Autophagy a cellular mechanism for disposal damaged organelles elimination intracellular pathogens. pivotal energy homeostasis plays critical roles...
Unresolved inflammation and tissue destruction are supposed to underlie the failure of dental pulp repair. As crucial regulators injury response, stem cells (DPSCs) play a key role in repair regeneration. M2 macrophages have been demonstrated induce osteogenic/odontogenic differentiation DPSCs. Ginsenoside Rb1 (GRb1) is major component ginseng manifested an anti-inflammatory by promoting M1 macrophage polarised into inflammatory disease. However, whether GRb1 facilitates odontogenic DPSCs...
Abstract Although combined antiretroviral therapy (ART) is successful in suppressing viral replication, HIV persists anatomic reservoirs, including the central nervous system (CNS). Current models, such as HSC-reconstituted humanized mice, lack matched human glia brain, limiting insights into CNS infection and pathogenesis. We developed a novel glia-immune mouse model integrating glial cells brain with donor-matched immune reconstitution peripheral blood lymphoid tissues. Neonatal NSG mice...
Type I interferons (IFN-Is) play a dual role in the immune response to HIV-1, providing early antiviral defense while driving dysfunction chronic phase. During acute infection, robust IFN signaling is critical controlling viral replication, activating innate immunity, and limiting reservoir establishment. However, sustained IFN-I activation during infection fuels systemic inflammation, exhaustion, fibrosis, particularly lymphoid tissues such as gut-associated tissue (GALT). Prolonged...
SIRT1, a mammalian ortholog of yeast silent information regulator 2 (Sir2), is an NAD+-dependent protein deacetylase that plays critical role in the regulation vascular function. The current study aims to investigate functional significance activity SIRT1 heart. Here we show early postnatal hearts expressed highest level compared adult and aged hearts. We generated transgenic mice with cardiac-specific expression dominant-negative form human (SIRT1H363Y), which represses endogenous activity....
Transfection is one of the most frequently used techniques in molecular biology that also applicable for gene therapy studies humans. One biggest challenges to investigate protein function and interaction have reliable monospecific detection reagents, particularly antibodies, all human products. Thus, a method can optimize transfection efficiency based on not only expression target interest but uptake nucleic acid plasmid, be an important tool biology. Here, we present simple, rapid robust...
Cannabis ( sativa ) is a widely used drug in the United States and frequency of cannabis use particularly high among people living with HIV (PLWH). One key component cannabis, non-psychotropic (−)-cannabidiol (CBD) exerts wide variety biological actions, including anticonvulsive, analgesic, anti-inflammatory effects. However, exact mechanism action through which CBD affects immune cell signaling remains poorly understood. Here we report that modulates type I interferon responses human...
Safe and efficient gene transfer systems are the basis of therapy applications. Non-integrating lentiviral (NIL) vectors among most promising candidates for tools, because they exhibit high efficiency in both dividing non-dividing cells do not present a risk insertional mutagenesis. However, non-integrating cannot introduce stable exogenous expression to cells, thereby limiting their application. Here, we report design vector that contains minimal scaffold/matrix attachment region (S/MAR)...
The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 ORF2, that code for an RNA-binding protein a with endonuclease reverse transcriptase activities, respectively. In this work, we examined the effects of full length L1-ORF2 ORF2 fragments on green fluorescent gene (GFP) expression when inserted into pEGFP-C1 vector downstream GFP. All sense...
Abstract Objectives The objectives of current study were to investigate the role and related mechanism Ginsenoside Rb1 (GRb1) on regulating apical periodontitis (AP) prognosis. Materials Methods Clinical specimens used determine involvement calcium overload‐induced macrophage pyroptosis in periapical tissues. Next, a ion‐chelating agent (BAPTA‐AM) was applied detect suppression intracellular overload pyroptosis. Then, network pharmacology, western blot (WB) analysis, Fluo‐4 assay conducted...
Chronic immune activation and inflammation are hallmarks of Human Immunodeficiency Virus-1 (HIV-1) pathogenesis. Therefore, approaches to safely reduce systematic essential improve responses thus slow or prevent HIV progression. Autophagy is a cellular mechanism for the disposal damaged organelles elimination intracellular pathogens. It not only vital energy homeostasis, but also plays critical role in regulating immunity. However, how it regulates antiviral T cell during infection unclear....
Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA cells and study gene protein function. Although several strategies have been developed, there wide variation with regards efficiency, toxicity reproducibility. Thus, sensitive robust method that can optimize efficiency based not only on expression of the target interest but also uptake nucleic acids, be an important tool biology. Herein, we present simple, rapid flow...
We investigated whether apolipoprotein A-I (apoA-I) mimetic peptides 4F and 6F can be a novel therapeutic strategy to reduce blood gut bioactive lipids, proinflammatory effects of endotoxin (LPS) aberrant activation cyclooxygenase 2 (COX-2) as instigators increased risk for cardiometabolic disease in chronic treated HIV.We used two humanized murine models HIV infection (n = 109 mice) explants from infected 10) persons determine Tg6F attenuate vivo ex lipids (measured by mass spectrometry)...
Gremlin1 is a multifunctional protein whose expression demonstrated to be involved in series of physiology and pathological processes. The association between apcial periodontitis (AP) has been established. M1-polarized macrophages are crucial immune cells that exacerbate the progression apical periodontal inflammatory response, but function during activation periapical lesions still unclear. This study attempts explore regulatory effects on macrophage polarization microenviroment.
ABSTRACT Chimeric Antigen Receptor (CAR) T cell therapy has emerged as a powerful immune for various diseases. Our studies in humanized mice and non-human primates (NHPs) demonstrate that hematopoietic stem (HSCs) modified with anti-HIV CAR leads to lifelong engraftment supply of functional anti-viral CAR-T cells, leading significantly reduced viral rebound after ART withdrawal. However, exhaustion, driven by chronic activation, remains major challenge the continuous efficacy therapy,...
The human immunodeficiency virus (HIV-1) pandemic continues to spread unabated worldwide, and currently, there is no vaccine available against HIV. Although combinational antiretroviral therapy (cART) has been successful in suppressing viral replication, it cannot completely eradicate the reservoir from HIV-infected individuals. A safe effective cure strategy for HIV infection will require multipronged methods, therefore advancements of animal models HIV-1 are pivotal development research....