A5046228019- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Marine animal studies overview
- Atherosclerosis and Cardiovascular Diseases
- HIV Research and Treatment
- Alzheimer's disease research and treatments
- Animal Vocal Communication and Behavior
- Protease and Inhibitor Mechanisms
- Bat Biology and Ecology Studies
- CRISPR and Genetic Engineering
- Lipid Membrane Structure and Behavior
University of California, Los Angeles
2019-2025
University of Washington
2016
Abstract Astrocytes are critical for the development and function of central nervous system. In developing brains, immature astrocytes undergo morphological, molecular, cellular, functional changes as they mature. Although mechanisms that regulate maturation other major cell types in system such neurons oligodendrocytes have been extensively studied, little is known about cellular molecular control astrocyte maturation. Here, we identified markers established an vitro assay studying Maturing...
Astrocytes are critical for the development and function of synapses. There notable species differences between human astrocytes commonly used animal models. Yet, it is unclear whether astrocytic genes involved in synaptic stable or exhibit dynamic changes associated with disease states age humans, which a barrier understanding astrocyte biology its potential involvement neurologic diseases. To better understand properties astrocytes, we acutely purified from cerebral cortices over 40 humans...
Within eukaryotic cells, translation is regulated independent of transcription, enabling nuanced, localized, and rapid responses to stimuli. Neurons respond transcriptionally translationally synaptic activity. Although transcriptional are documented in astrocytes, here we test whether astrocytes have programmed translational responses. We show that seizure activity rapidly changes the transcripts on astrocyte ribosomes, some predicted be downstream BDNF signaling. In acute slices, quantify...
Abstract Although combined antiretroviral therapy (ART) is successful in suppressing viral replication, HIV persists anatomic reservoirs, including the central nervous system (CNS). Current models, such as HSC-reconstituted humanized mice, lack matched human glia brain, limiting insights into CNS infection and pathogenesis. We developed a novel glia-immune mouse model integrating glial cells brain with donor-matched immune reconstitution peripheral blood lymphoid tissues. Neonatal NSG mice...
ABSTRACT In response to central nervous system injury or disease, astrocytes become reactive, adopting context-dependent states and functional outputs. Certain inflammatory insults induce reactive that lose homeostatic functions gain harmful outputs through cellular pathways are not fully understood. Here, we combined single-cell transcriptomics with CRISPRi screening in human iPSC-derived systematically interrogate astrocyte reactivity. We found autocrine-paracrine IL-6 interferon signaling...
Abstract Astrocytes are dynamic cells with important roles in brain function and neurological disease. There notable species differences between human astrocytes commonly used animal models. However, changes of the molecular attributes across disease states, sex, age largely unknown, which is a barrier understanding astrocyte biology its potential involvement diseases. To better understand properties astrocytes, we acutely purified from cerebral cortices over 40 humans various ages, sexes,...
Though the brain was long characterized as an immune-privileged organ, findings in recent years have shown extensive communications between and peripheral immune cells. We now know that alterations system can affect behavioral outputs of central nervous system, but we do not which cells are affected by presence Glial including microglia, astrocytes, oligodendrocytes, oligodendrocyte precursor (OPCs) critical for development function system. In a wide range neurological psychiatric diseases,...