A5107980711- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
- Electrospun Nanofibers in Biomedical Applications
- Hair Growth and Disorders
- Pancreatic function and diabetes
- Bone Tissue Engineering Materials
- Skin and Cellular Biology Research
- 3D Printing in Biomedical Research
- Diabetes Treatment and Management
- Drug Transport and Resistance Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Regulation of Appetite and Obesity
- Genetics and Neurodevelopmental Disorders
- Diabetes Management and Research
- Veterinary Oncology Research
- Neurological Disease Mechanisms and Treatments
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Sarcoma Diagnosis and Treatment
- Magnesium in Health and Disease
Vanderbilt University
2021-2025
Kyungpook National University
2024-2025
Konkuk University
2019-2024
Konkuk University Medical Center
2022-2024
AstraZeneca (Japan)
2016-2022
Ajou University
2021
Republic of Korea Army
2021
AstraZeneca (Brazil)
2017-2020
Rutgers, The State University of New Jersey
2016-2019
Wonkwang University
2014
Abstract Astrocytes are critical components of the neurovascular unit that support blood-brain barrier (BBB) function. Pathological transformation astrocytes to reactive states can be protective or harmful BBB Here, using a human induced pluripotent stem cell (iPSC)-derived co-culture model, we show tumor necrosis factor (TNF) transitions an inflammatory state causes dysfunction through activation STAT3 and increased expression SERPINA3 , which encodes alpha 1-antichymotrypsin (α1ACT). To...
The process of oligodendrogenesis has been relatively well delineated in the rodent brain. However, it remains unknown whether analogous developmental processes are manifested human Here we report forebrain organoids, generated by using OLIG2-GFP knockin pluripotent stem cell (hPSC) reporter lines. OLIG2/GFP exhibits distinct temporal expression patterns ventral organoids (VFOs) versus dorsal (DFOs). Interestingly, can be induced both VFOs and DFOs after neuronal maturation. Assembling to...
The mammalian tissue extracellular matrix (ECM) has been used as a scaffold to facilitate the repair and reconstruction of numerous tissues. However, material properties decellularized ECM (dECM) from in vitro cell cultures effect these on wound remodeling remain unclear. To elucidate its biological activity, we extracted dECM human lung fibroblasts, fabricated it into patch, applied full-thickness skin wound. fibroblast-derived (fdECM) maintained content collagen Ⅰ, Ⅳ, elastin, extraction...
Abstract Aim To evaluate the safety and pharmacokinetics of cotadutide, a dual glucagon‐like peptide‐1 (GLP‐1) glucagon receptor agonist, in overweight Asian participants with or without type 2 diabetes (T2D). Materials Methods In phase 1, randomized, blinded, single‐ascending dose study, 24 Japanese eight Chinese healthy adults (body mass index [BMI] 23‐40 kg/m ) received one subcutaneous cotadutide (50‐150 100 μg, respectively) placebo. The primary endpoint was safety. 2a, double‐blinded,...
<i>Background:</i> As of 2010, the rivastigmine patch was licensed for treatment Alzheimer’s disease (AD) in 64 countries. <i>Methods:</i> This 24-week, multicenter, randomized, double-blind, placebo-controlled study evaluated efficacy, safety and tolerability 5-cm<sup>2</sup> (9-mg loading dose; 4.6 mg/24 h delivery rate) 10-cm<sup>2</sup> (18-mg 9.5 Japanese patients with AD. <i>Results:</i> In primary analysis population...
Here we show that intradermal injection of keratin promotes hair growth in mice, which results from extracellular interaction with forming cells. Extracellular application induces condensation dermal papilla cells and the generation a P-cadherin-expressing cell population (hair germ) outer root sheath via keratin-mediated microenvironmental changes. Exogenous is reflected by finding exposure transforming factor beta 2 (TGFβ2)-induced apoptotic appears to be critical for germ formation....
This study investigated the potential of indirubin-3′-oxime (I3O) compound to mitigate temperature-induced male infertility in Drosophila melanogaster. Elevated temperatures significantly reduced egg-hatching rates, but I3O supplementation improved these suggesting it can partially restore fertility under heat stress. Additionally, was found inhibit soluble epoxide hydrolase (sEH), an enzyme involved metabolism epoxyeicosatrienoic acids, which are vital for reproductive health. exhibited sEH...
Aims To evaluate the efficacy and safety of dapagliflozin as add‐on to insulin in J apanese patients with type 2 diabetes. Materials methods Insulin‐treated were randomized 5 mg or placebo during a 16‐week double‐blind treatment period. Both groups then received 10 (the dose was increased at after week 24 if glycated haemoglobin [ HbA1c ] previous visit >7.5%) 36‐week open‐label extension The exploratory endpoint assess maintenance 5/10 + over 52 weeks treatment. Safety assessed terms...
Brain endothelial cells experience mechanical forces in the form of blood flow-mediated shear stress and underlying matrix stiffness, but intersectional contributions these factors towards blood-brain barrier (BBB) impairment neurovascular dysfunction have not been extensively studied. Here, we developed vitro models to examine sensitivity primary human brain microvascular (BMECs) substrate with or without exposure fluid stress. Using a combination molecular profiling techniques, show that...
Historically, studying the development of brain and central nervous system (CNS) tissues has been challenging. Human pluripotent stem cell (hPSC) technology allowed for in vitro reconstitution relevant, early trajectories by using small molecules recombinant proteins to guide differentiation cells toward relevant CNS phenotypes. However, many these protocols fail recapitulate cell-guided programs intrinsic embryonic development, particularly signaling centers that emerge within neural tube...
This report describes two cases of solitary subcutaneous nodular lymphoid lesions in dogs. Case 1 involved a 6-year-old male Maltese and 2 5-year-old female Yorkshire Terrier. Both presented with firm, non-ulcerated dorsal nodules were unresponsive to corticosteroids. Surgical excision was performed for diagnosis. Histopathology immunohistochemistry revealed distinct patterns. exhibited well-formed follicles CD20+/PAX5+ B cells strong BCL6 but absent BCL2 expression, consistent B-cell...
The ATP-binding cassette subfamily B member 1 (ABCB1), encoding a multidrug transporter referred to as P-glycoprotein (Pgp), plays critical role in the efflux of xenobiotics humans and is implicated cancer resistance chemotherapy. Therefore, developing high-throughput animal models screen for Pgp function bioavailability substrates inhibitors paramount. Here, we generated validated zebrafish knockout line abcb4, human homolog. CRISPR/Cas9 genome editing technology was deployed generate...
Axo-glial interactions are critical for myelination and the domain organization of myelinated fibers. Cell adhesion molecules belonging to Cadm family, in particular Cadm3 (axonal) its heterophilic binding partner Cadm4 (Schwann cell), mediate these along internode. Using targeted shRNA-mediated knockdown, we show that removal axonal promotes Schwann cell vitro DRG neuron/Schwann myelinating system. Conversely, over-expressing on surface neuron axons results an almost complete inability by...
ABSTRACT In response to central nervous system injury or disease, astrocytes become reactive, adopting context-dependent states and functional outputs. Certain inflammatory insults induce reactive that lose homeostatic functions gain harmful outputs through cellular pathways are not fully understood. Here, we combined single-cell transcriptomics with CRISPRi screening in human iPSC-derived systematically interrogate astrocyte reactivity. We found autocrine-paracrine IL-6 interferon signaling...
4075 Background: TOPAZ-1 (NCT03875235) is a randomized, double-blind, global, Phase 3 study evaluating the efficacy and safety of D + GC as first-line treatment for patients (pts) with advanced BTC. significantly improved overall survival (OS) versus placebo (PBO) (Oh D-Y, et al. J Clin Oncol 2022;40[suppl 4]. Abs 378). Methods: A pre-specified subgroup analysis was performed outcomes including OS pts enrolled in Asia (China, Hong Kong, India, Japan, South Korea, Taiwan, Thailand) or rest...
A bstract Excessive brain iron accumulation is observed early in the onset of Alzheimer's disease, notably prior to widespread proteinopathy. These findings suggest that increases levels are due a dysregulation transport mechanism at blood–brain barrier. Astrocytes release signals (apo‐ and holo‐transferrin) communicate needs endothelial cells order modulate transport. Here we use iPSC‐derived astrocytes investigate how early‐disease amyloid‐β disrupt secreted by stimulate from cells. We...
Intervertebral disc degeneration (IVDD) is a common cause of lower back pain. Programmed cell death (PCD) including apoptosis and autophagy known to play key mechanistic roles in the development IVDD. We hypothesized that nucleus pulposus cells make up center IVD can be affected by aging environmental oxygen concentration, thus affecting Here, we evaluated phenotype changes PCD signaling two different percentages (5% (hypoxia) 20% (normoxia)) serial passage 20. NP were isolated from lumbar...
Tendons are responsible for transmitting mechanical forces from muscles to bones body locomotion and joint stability. However, tendons frequently damaged with high forces. Various methods have been utilized repairing tendons, including sutures, soft tissue anchors, biological grafts. experience a higher rate of retear post-surgery due their low cellularity vascularity. Surgically sutured vulnerable reinjury inferior functionality when compared native tendons. Surgical treatment using grafts...
In human Alzheimer's disease (AD), the aggregation of tau protein is considered a significant hallmark, along with amyloid-beta. The formation neurofibrillary tangles due to aberrant phosphorylation disrupts microtubule stability, leading neuronal toxicity, dysfunction, and subsequent cell death. Nesfatin-1 neuropeptide primarily known for regulating appetite energy homeostasis. However, function in neuroprotective role has not been investigated. this study, we aimed elucidate effect on...