A5064258729- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Hedgehog Signaling Pathway Studies
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- NF-κB Signaling Pathways
- Neuroblastoma Research and Treatments
- Genomic variations and chromosomal abnormalities
- Cancer-related gene regulation
- Congenital Ear and Nasal Anomalies
- RNA regulation and disease
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Cancer and Skin Lesions
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Genetic factors in colorectal cancer
- Natural product bioactivities and synthesis
- melanin and skin pigmentation
- Epigenetics and DNA Methylation
- Insect and Pesticide Research
Penn State Milton S. Hershey Medical Center
2015-2024
Pennsylvania State University
2016-2024
University of Wisconsin Carbone Cancer Center
2007-2023
University of Wisconsin–Madison
2009-2023
Hershey (United States)
2015-2018
Pediatric Oncology Group
2018
Madison Group (United States)
2015
Cancer Institute (WIA)
2008-2014
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2011
University of Pennsylvania
2011
We previously showed that the calcium-binding protein S100A4 is overexpressed during progression of prostate cancer (CaP) in humans and TRAMP (transgenic adenocarcinoma mouse prostate) model. tested a hypothesis gene plays role invasiveness human CaP may be associated with its metastatic spread. observed siRNA-mediated suppression significantly reduced proliferative invasive capability highly cells PC-3. evaluated mechanism through which controls by using macroarray containing 96 well...
Insulin-like growth factor 2 mRNA-binding protein-1 (IMP-1) is an oncofetal protein that binds directly to and stabilizes oncogenic c-Myc regulates, in turn, its posttranscriptional expression translation. In contrast normal adult tissue, IMP-1 reexpressed and/or overexpressed human cancers. We show knockdown of colon cancer cell lines increases the mature let-7 miRNA family members downregulates several mRNA targets: IMP-1, Cdc34, K-Ras. further loss inhibits Lin-28B, K-Ras, suppresses...
Iron is essential for normal brain development and function. Hence, understanding the mechanisms of iron efflux at blood-brain barrier their regulation are critical establishment homeostasis. Here, we have investigated role exosomes in mediating transfer H-ferritin (FTH1)- or transferrin (Tf)-bound across endothelial cells (BBBECs). Our study used ECs derived from human-induced pluripotent stem that grown bicameral chambers. When were exposed to 55Fe-Tf 55Fe-FTH1, 55Fe activity exosome...
Insulin-like growth factor 2 mRNA-binding proteins (IGF2BP1, IGF2BP2, and IGF2BP3) are a family of RNA-binding that play an essential role in the development disease by regulating mRNA stability translation critical regulators cell division metabolism. Genetic chemical inhibition these slows down cancer proliferation, decreases invasiveness, prolongs life span variety animal models. The induction tissues' immunogenicity is increasingly recognized, but, impact IGF2BPs on innate adaptive...
The Hedgehog (Hh) signaling pathway plays a crucial role in embryogenesis and has been linked to the development of several human malignancies. transcription factor Gli2 key transduction Hh signals by modulating some target genes, yet mechanisms that control protein expression are largely unknown. Here we report β-transducin repeat-containing (β-TrCP) E3 ubiquitin ligase is required for degradation. β-TrCP2 directly binds wild type promotes its ubiquitination. Single amino acid substitution...
Wnt and Hedgehog signaling pathways play central roles in embryogenesis, stem cell maintenance, tumorigenesis. However, the mechanisms by which these two interact are not well understood. Here, we identified a novel mechanism pathway stimulates transcriptional output of signaling. Wnt/beta-catenin induces expression an RNA-binding protein, CRD-BP, turn binds stabilizes GLI1 mRNA, causing elevation activity. The newly described mode regulation seems to be important several functions Wnt,...
Igf2 mRNA binding protein 1 (IMP1, CRD-BP, ZBP-1) is a messenger RNA that we have shown previously to regulate colorectal cancer (CRC) cell growth in vitro . Furthermore, increased IMP1 expression correlates with enhanced metastasis and poor prognosis CRC patients. In the current study, sought elucidate IMP1-mediated functions pathogenesis vivo Using xenografts, demonstrate overexpression promotes xenograft tumor dissemination into blood. intestine-specific knockdown of Imp1 dramatically...
Igf2bp1 is an oncofetal RNA binding protein whose expression in numerous types of cancers associated with upregulation key pro-oncogenic RNAs, poor prognosis, and reduced survival. Importantly, synergizes mutations Kras to enhance signalling oncogenic activity, suggesting that molecules inhibiting could have therapeutic potential. Here, we isolate a small molecule interacts hydrophobic surface at the boundary KH3 KH4 domains, inhibits RNA. In cells, compound reduces level other mRNA targets,...
Background Anti-GD2 monoclonal antibody immunotherapy has significantly improved the overall survival rate for high-risk neuroblastoma patients. However, 40% of patients fail to respond or develop resistance treatment, and molecular mechanisms by which this occurs remain poorly understood. Tumor-derived small extracellular vesicles (sEVs) have emerged as critical regulators in modulating response immunotherapy. In study, we investigated role neuroblastoma-derived sEVs promoting anti-GD2...
Aberrant activation of the Hedgehog (Hh) signaling pathway has been reported in various cancer types including prostate cancer. The GLI2 transcription factor is a primary mediator Hh signaling. However, its relative contribution to development tumors poorly understood. To establish role maintaining tumorigenic properties cells, we developed GLI2-specific small hairpin RNA. Knockdown these cells resulted significant down-regulation pathway, followed by inhibition colony formation,...
Wingless (Wnt) signaling pathway regulates a variety of cellular processes including proliferation, differentiation, survival, apoptosis and cell motility.Aberrant activation Wnt/b-catenin has been observed in approximately one-third melanomas this subset very poor prognosis suggesting that targeting Wnt could be promising strategy against subtype.Mel 928 Mel 1241 melanoma cells representative with constitutive 1011 lack were treated dietary triterpene lupeol its effects on growth, b-catenin...
Alternative cleavage and polyadenylation generates multiple transcript variants producing mRNA isoforms with different length 3′-UTRs. enables differential post-transcriptional regulation via the availability of cis-acting elements in Microphthalmia-associated transcription factor (MITF) is a master regulator melanocyte development melanogenesis. This central also implicated melanoma development. Here, we show that cells favor expression MITF shorter 3′-UTR. We establish this isoform...