A5007703186- Extracellular vesicles in disease
- Retinal Diseases and Treatments
- Biomarkers in Disease Mechanisms
- Circular RNAs in diseases
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Barrier Structure and Function Studies
- Connective tissue disorders research
- Connexins and lens biology
- MicroRNA in disease regulation
- Retinal and Optic Conditions
- Neuroblastoma Research and Treatments
- Stress Responses and Cortisol
- Ocular Diseases and Behçet’s Syndrome
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neurological Disease Mechanisms and Treatments
- Mosquito-borne diseases and control
- Ocular Oncology and Treatments
- Neurotransmitter Receptor Influence on Behavior
- Cancer-related molecular mechanisms research
- Metabolomics and Mass Spectrometry Studies
- Salivary Gland Disorders and Functions
- Cell Image Analysis Techniques
- Cleft Lip and Palate Research
Pennsylvania State University
2011-2025
Penn State Milton S. Hershey Medical Center
2015-2024
University of Michigan
2013-2024
Michigan Medicine
2024
W.K. Kellogg Foundation
2018-2021
Michigan United
2020-2021
Hershey (United States)
2018
Northeastern University
2002
National Institutes of Health
2001
National Institute of Mental Health
1998-2000
purpose. VEGF is a potent permeabilizing factor that contributes to the pathogenesis of diabetic retinopathy and brain tumors. VEGF-induced vascular permeability in vivo cell culture requires PKC activity, but mechanism by which regulates barrier properties remains unknown. This study was conducted examine how diabetes alter occludin phosphorylation endothelial permeability. methods. Chemical inhibitors activators were used treat primary retinal cells culture. In vitro kinase assays Western...
Purpose: Current evidence suggests that retinal neurodegeneration is an early event in the pathogenesis of diabetic retinopathy. Our main goal was to examine whether, human retina, common proteins and pathways are shared with brain neurodegenerative diseases. Methods: A proteomic analysis performed on three groups postmortem retinas matched by age: nondiabetic control (n = 5), without glial activation 5). Retinal lysates from each group were pooled run SDS-PAGE gel. Bands analyzed...
To investigate the molecular components of vitreous in order to better understand retinal physiology and disease.Vitreous was acquired from patients undergoing vitrectomy for macular hole and/or epiretinal membrane, postmortem donors, C57BL/6J mice. Unbiased proteomic analysis performed via electrospray ionization tandem mass spectrometry (MS/MS). Gene ontology results were confirmed with transmission electron microscopy, atomic force nanoparticle tracking (NTA).Proteomic obtained prior...
// Oliver D. Mrowczynski 1 , Achuthamangalam B. Madhankumar Jeffrey M. Sundstrom 2 Yuanjun Zhao Yuka Imamura Kawasawa 3 Becky Slagle-Webb Christine Mau Russell A. Payne Elias Rizk Brad E. Zacharia and James R. Connor Department of Neurosurgery, Pennsylvania State University College Medicine, Hershey, PA 17033, USA Ophthalmology, Pharmacology, Biochemistry Molecular Biology, Institute for Personalized Correspondence to: Mrowczynski, email: omrowczynski@pennstatehealth.psu.edu Keywords:...
Background Anti-GD2 monoclonal antibody immunotherapy has significantly improved the overall survival rate for high-risk neuroblastoma patients. However, 40% of patients fail to respond or develop resistance treatment, and molecular mechanisms by which this occurs remain poorly understood. Tumor-derived small extracellular vesicles (sEVs) have emerged as critical regulators in modulating response immunotherapy. In study, we investigated role neuroblastoma-derived sEVs promoting anti-GD2...
The molecular function of occludin, an integral membrane component tight junctions, remains unclear. VEGF-induced phosphorylation sites were mapped on occludin by combining MS data analysis with bioinformatics. In vivo Ser490 was validated and protein interaction studies combined crystal structure suggest that attenuates the between ZO-1. This study demonstrates bioinformatics can successfully identify novel from limiting samples.
To evaluate the imaging features of choroidal metastasis using enhanced depth optical coherence tomography (EDI-OCT).Enhanced 24 metastatic tumors with <2.5 mm thickness on ultrasonography were retrospectively evaluated.Of tumors, 10 (42%) located at macula and 14 (58%) between equator. Tumor was plateau-shaped in 18 (75%) dome-shaped 6 (25%) tumors. On EDI-OCT, showed low internal reflectivity 17 (71%) high 7 (29%) Most common associated overlying choriocapillaris thinning (100%) shaggy or...
Pro-inflammatory cytokines and growth factors such as VEGF (vascular endothelial factor) contribute to the loss of BRB (blood-retinal barrier) subsequent macular oedema in various retinal pathologies. signalling requires PKCβ [conventional PKC (protein kinase C)] activity; however, inhibition only partially prevents VEGF-induced permeability does not affect pro-inflammatory cytokine-induced permeability, suggesting involvement alternative pathways. In present study, we provide evidence for...
Abstract Although neoadjuvant chemotherapy is a standard component of breast cancer treatment, recent evidence suggests that chemotherapeutic drugs can promote metastasis through poorly defined mechanisms. Here we utilize xenograft mouse models triple-negative to explore the importance chemotherapy-induced tumor-derived small extracellular vesicles (sEV) in metastasis. Doxorubicin (DXR) enhanced tumor cell sEV secretion accelerate pulmonary by priming premetastatic niche. Proteomic analysis...
Tight junctions (TJs) are dynamic cellular structures that critical for compartmentalizing environments within tissues and regulating transport of small molecules, ions, fluids. Phosphorylation-dependent binding the transmembrane protein occludin to structural organizing ZO-1 contributes regulation barrier properties; however, details their interaction controversial. Using angle X-ray scattering (SAXS), NMR chemical shift perturbation, cross-saturation, in vitro binding, site-directed...
Purpose: The goals of this study were to evaluate the safety office-based vitreous sampling, and determine utility these samples with multiplex cytokine analysis. Methods: Vitreous collected from needle aspiration rate adverse events during follow-up was reviewed. concentrations in a subset patients diabetic macular edema (DME) analyzed using 42 plex-cytokine bead array. These results compared proliferative retinopathy (PDR) controls (macular hole, epiretinal membrane, symptomatic floaters)...
Human cytomegalovirus (HCMV) is a herpesvirus that leads to serious health consequences in neonatal or immunocompromised patients. Clinical management of infection these at-risk groups remains concern even with approved antiviral therapies available. It necessary increase our understanding the cellular changes occur during and their importance virus spread. This may help identify new targets will lead development novel treatment strategies. Extracellular vesicles (EVs) represent an important...
Proper control of cell cycle progression and barrier function are essential processes to the maintenance epithelial homeostasis. The contribution tight junction proteins is well established, whereas their only beginning be understood. Centrosomes principal microtubule organizing centers in eukaryotic cells centrosome duplication separation linked mitotic entry. Here we demonstrate that occludin localizes with centrosomes Madin-Darby canine kidney cells. Immunocytochemistry biochemical...
The 2024 Mary Tyler Moore Vision Initiative (MTM Vision) Workshop on Data convened to discuss best practices and specific considerations for building a comprehensive, shareable MTM data lake. workshop aimed accelerate the development of new indications, therapies, regulatory pathways diabetic retinal disease (DRD) by standardizing harmonizing clinical ocular 'omics analyses. Standardization collection, use common elements, interoperability were emphasized, alongside federated learning...