A5100321879- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Cardiac Fibrosis and Remodeling
- Additive Manufacturing and 3D Printing Technologies
- Pluripotent Stem Cells Research
- Neurological and metabolic disorders
- Graphene and Nanomaterials Applications
- Cellular Mechanics and Interactions
- Signaling Pathways in Disease
- Congenital heart defects research
- FOXO transcription factor regulation
- Cardiac Structural Anomalies and Repair
- Electrolyte and hormonal disorders
- Heparin-Induced Thrombocytopenia and Thrombosis
- Muscle and Compartmental Disorders
- Antimicrobial Peptides and Activities
- Ear Surgery and Otitis Media
- Epigenetics and DNA Methylation
- Apelin-related biomedical research
- Bone Tissue Engineering Materials
- Hearing, Cochlea, Tinnitus, Genetics
- Viral Infections and Immunology Research
- Diabetes Management and Research
New Jersey Institute of Technology
2013-2024
University of California, San Diego
2024
Jeonbuk National University
2024
National University Health System
2021
Hudson Institute
2016-2019
John Wiley & Sons (United Kingdom)
2019
Lustgarten Foundation
2017
Cold Spring Harbor Laboratory
2017
National Center for Genetic Engineering and Biotechnology
2016
John Wiley & Sons (United States)
2016
Because adult lung tissue has limited regeneration capacity, transplantation is the primary therapy for severely damaged lungs. To explore whether can be regenerated in vitro, we treated lungs from rats using a procedure that removes cellular components but leaves behind scaffold of extracellular matrix retains hierarchical branching structures airways and vasculature. We then used bioreactor to culture pulmonary epithelium vascular endothelium on acellular matrix. The seeded displayed...
Maintenance of normal myocardial function depends intimately on synchronous tissue contraction, driven by electrical activation and adequate nutrient perfusion in support thereof. Bioreactors have been used to mimic aspects these factors vitro engineer cardiac but, due design limitations, previous bioreactor systems yet simultaneously perfusion, stimulation unconstrained (i.e. not isometric) contraction. To the best our knowledge, system described herein is first integrate three key concert....
Recently, electrospun polyvinylidene fluoride (PVDF) and fluoride-trifluoroethylene (PVDF-TrFE) scaffolds have been developed for tissue engineering applications. These materials piezoelectric activity, wherein they can generate electric charge with minute mechanical deformations. Since the myocardium is an electroactive tissue, unique feature of a scaffold attractive cardiovascular In this study, we examined cytocompatibility function pluripotent stem cell derived cells including mouse...
Electrical and mechanical anisotropy arise from matrix cellular alignment in native myocardium. Generation of engineered heart tissue will be required to match properties provide immediate opportunities investigate the genesis structural determinants functional anisotropy. We investigated influence geometry boundary conditions on fibroblast thin collagen gels. Consistent with previous reports, we found that human dermal fibroblasts align parallel free edges partially constrained gels;...
Abstract Contractile behavior of cardiomyocytes relies on directed signal propagation through the electroconductive networks that exist within native myocardium. Due to their unique electrical properties, electroactive materials, such as graphene, have recently emerged promising candidate materials for cardiac tissue engineering applications. In this work, potential three‐dimensional (3D) nanofibrous graphene and poly(caprolactone) (PCL + G) composite scaffold has been explored first time....
A growing area in the field of tissue engineering is development equivalents as model systems for vitro experimentation and high-throughput screening applications. Although a variety strategies have been developed to enhance structure function engineered cardiac tissues, an inherent limitation with traditional myocardial patches that they do not permit evaluation fundamental relationships between pressure volume characterize global contractile heart. Therefore, following study we introduce...
The requirements for engineering clinically sized cardiac constructs include medium perfusion (to maintain cell viability throughout the construct volume) and protection of myocytes from hydrodynamic shear. To reconcile these conflicting requirements, we proposed use porous elastomeric scaffolds with an array channels providing conduits perfusion, to provide efficient transport oxygen cells, by a combination convective flow molecular diffusion over short distances between channels. In this...
Advancements in regenerative medicine have highlighted the potential of decellularized extracellular matrix (ECM) as a scaffold for organ bioengineering. Although ECM major systems is well-recognized, studies focusing on angiogenic effects pancreatic are limited. This study investigates capabilities ECM, particularly its role promoting angiogenesis. Using Triton-X-100 solution, porcine pancreas was successfully decellularized, resulting significant reduction DNA content (97.1% removal) while...
Engineering solid tissues, including cardiac muscle, requires the inclusion of a microvasculature. Prevascularization in vitro will likely be dependent upon coculturing parenchymal cells with vascular cells, on matrix that is sufficiently porous to allow microvessel formation. In this study, we examined behavior and function endothelial highly elastomeric 3D poly(glycerol sebacate) (PGS) scaffold, provide flexible biocompatible cell delivery system for developing engineered tissues...
Following myocardial infarction (MI), inflammation plays a crucial role in the pathogenesis of MI injury and macrophages are among key cells activated during initial phases host response regulating healing process. While have emerged as attractive effectors tissue repair, contribution on cardiac cell function survival is not fully understood due to complexity vivo inflammatory microenvironment. Understanding involved how they communicate with one another paramount importance for development...
Heart disease is one of the leading causes death in world. There a growing demand for vitro cardiac models that can recapitulate complex physiology tissue. These provide platform to better understand underlying mechanisms development and aid developing novel treatment alternatives platforms towards personalized medicine. In this review, summary engineered presented. Basic design considerations replicating heart's microenvironment are discussed considering anatomy heart. This followed by...
Myocardial infarction (MI) is a major cause of morbidity and mortality worldwide, especially in aging metabolically unhealthy populations. A target regenerative tissue engineering the restoration viable cardiomyocytes to preserve cardiac function circumvent progression heart failure post-MI. Amelioration ischemia crucial component such restorative strategies. Angiogenic β-sheet peptides can self-assemble into thixotropic nanofibrous hydrogels. These syringe aspiratable cytocompatible gels...
For efficiently assessing the potential for grafted cells to repair infarcted myocardium, a simplified surrogate heart muscle system would offer numerous advantages. Using neonatal rat cardiac myocytes in collagen matrix, we created thin cylindrical engineered tissues (ECTs) that exhibit essential aspects of physiologic function. Furthermore, novel cryo-injured ECT model myocardial infarction offers longitudinal study mechanisms cell-based vitro.
The islet endothelium comprises a specialized population of endothelial cells (IECs) expressing unique markers such as nephrin and α-1 antitrypsin (AAT) that are not found in surrounding tissues. However, due to difficulties isolating maintaining pure these cells, the information on islet-specific is currently very limited. Interestingly, we have identified large subpopulation exhibiting IEC phenotype, while deriving insulin-producing from mouse embryonic stem (mESCs). These were by uptake...
Although structural anisotropy is important for proper function of normal myocardium, it has been a challenge to mimic architecture native tissue in vitro. Previous studies have focused on using two-dimensional cultures utilizing micropatterning substrates. Here, we present three-dimensional (3D) decellularized engineered tissues with controlled varying degree extracellular matrix alignment. Engineered were initially created by embedding cardiac fibroblasts 3D collagen (rat tail type I...
Background A number of drugs are toxic to the cochlear sensory cells known as hair (HCs), resulting in hearing loss. Treatment with survival-promoting growth factors, antioxidants, and inhibitors cell death pathways or proteinases have been shown reduce HC damage vivo and/or vitro animal models. Conversely, translation humans has often disappointing. This may be due complexity intracellular processes. We hypothesized that combining treatments targeting different cellular processes would more...
This fi gure shows an artistic representation of UVC inactivation virus particles in a plastic fl uoropolymer microcapillary lm.The lm has refractive index similar to water and is very transparent light, consisting number parallel microcapillaries embedded at inner diameter the tuned for rapid effi cient continuous ows.The cover image, by Nuno Miguel Reis et al., based on Article Photo microfl uidic capillary systems,
Rhabdomyolysis is the consequence of extensive muscle injury with release cell constituents into plasma.It can arise from trauma and also a variety nontraumatic causes.Trauma, drugs, toxins infection are major causes rhabdomyolysis, but it rarely associated metabolic disorders such as severe electrolyte disturbance, diabetic ketoacidosis, hyperosmolar nonketotic coma, hypothyroidism thyrotoxicosis.There have been several reported cases panhypopituitarism cause has never identified.We...
For efficiently assessing the potential for grafted cells to repair infarcted myocardium, a simplified surrogate heart muscle system would offer numerous advantages. Using neonatal rat cardiac myocytes in collagen matrix, we created thin cylindrical engineered tissues (ECTs) that exhibit essential aspects of physiologic function. Furthermore, novel cryo-injured ECT model myocardial infarction offers longitudinal study mechanisms cell-based vitro
To circumvent the lack of donor pancreas, insulin-producing cells (IPCs) derived from pluripotent stem emerged as a viable cell source for treatment type 1 diabetes. While it has been shown that IPCs can be using various protocols, long-term viability and functional stability in vitro remains challenge. Thus, principles three-dimensional (3D) tissue engineering perfusion flow bioreactor were used this study to establish 3D microenvironment suitable culture IPCs-derived mouse embryonic cells....