A5067379892- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Hepatitis Viruses Studies and Epidemiology
- HIV/AIDS Research and Interventions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Bacteriophages and microbial interactions
- HIV, Drug Use, Sexual Risk
- Viral gastroenteritis research and epidemiology
- Liver Diseases and Immunity
- Pneumocystis jirovecii pneumonia detection and treatment
- Immunodeficiency and Autoimmune Disorders
- Blood disorders and treatments
- SARS-CoV-2 and COVID-19 Research
- Mast cells and histamine
- COVID-19 Clinical Research Studies
- Dermatological and COVID-19 studies
- Leptospirosis research and findings
- HIV, TB, and STIs Epidemiology
- Blood groups and transfusion
- Autoimmune Bullous Skin Diseases
- Cytomegalovirus and herpesvirus research
- Infections and bacterial resistance
Saint Petersburg Pasteur Institute
2016-2025
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2020-2023
Institut Pasteur
2015
Russian Academy of Sciences
2011
The aim of the study was to search for candidate genes interacting with HIV attachment receptors (CCR5, CXCR4, CCR2, CD4) and potentially involved in disease pathogenesis, based on complex silico network algorithms. Materials methods. A number web applications were used analyse genetic protein-protein networks, algorithms databases which are complementary. CD4 receptor chemokine co-receptor CCR5, CXCR4 CCR2 as background/baseline all cases, their protein products play a key role process...
Background: Vietnam has made significant strides in reducing the prevalence of HIV infection and achievements its antiretroviral treatment program. However, COVID-19 pandemic financial challenges healthcare system have posed obstacles to maintaining effective monitoring, particularly among vulnerable populations. This study aims evaluate situation drug resistance patients who experienced failure Mekong Delta region compare data from 2019 2022. Methods: The material was blood plasma samples...
Abstract The problem of viral hepatitis A (HA) and E (HE), has remained relevant for many decades. According to estimates by the World Health Organization, millions people are exposed annually risk infection with these (HAV) (HEV) viruses. Because HA HE can be asymptomatic, their true prevalence is very difficult estimate. aim was assess seroprevalence HAV HEV infections in Kyrgyz Republic, taking into account infectious vaccination status socio-demographic characteristics volunteers....
Introduction. The problem of transfusion safety in relation to parenteral viral hepatitis still remains relevant. Viral B (HB) the most common infection transmitted through procedures. One natural phases chronic (CHB) is occult (OBI), characterized by an undetectable HBsAg (regardless other serological markers content) presence virus (HBV) DNA liver tissue and extremely low, up undetectable, level load blood. In Republic Guinea, as countries on continent, prevention HBV transmission based...
Healthcare workers are much more likely to be infected with HIV and hepatitis viruses compared the general population. Although healthcare aware of viruses, several countries in Africa lack a comprehensive grasp disease routes transmission risks. The aim this study was assess prevalence serological molecular biological markers viral among Republic Guinea. material 74 blood serum samples collected from who received additional training at Institute Applied Biological Research Guinea (IRBAG,...
The aim of the study was to identify prevalence drug resistance mutations in hepatitis C virus among patients with relapse disease on therapy direct antiviral drugs. Materials and methods. material included 31 blood plasma samples from chronic Samples were screened for presence HCV RNA. In case detection RNA, amplification carried out using a set primers jointly flanking NS3, NS5A, NS5B genes. After sequencing nucleotide sequences these genes, subtype determined identified. Results...
Leptospirosis is a zoonotic disease found virtually worldwide. Microscopic Agglutination Test with live leptospira (MAT) the reference method for serological diagnosis of leptospirosis. MAT based on assessing serum potential to agglutinate serovar Leptospira maintained at laboratory. At some laboratories having own collections isolated and strains applicable diagnosis, those microorganisms are many years by repeated subculturing, that increases markedly chance strain cross-contamination. The...
To analyze the method for detecting HBV DNA in peripheral blood at low viral load and evaluate its significance identifying HBsAg-negative hepatitis B. In this work, samples of liver tissue biopsy material were used from 128 patients living Russian Federation Republic Uzbekistan without CHB with confirmed detection circle covalently closed hepatocytes. Plasma was measured using «AmpliSens® HBV-Monitor-FL» kit. detected by nested PCR. Analytical sensitivity checked step dilution. According to...
Introduction. The immune status is a multifaceted parameter quantitatively and qualitatively analyzing functional activity system state in organs as well some non-specific mechanisms of antimicrobial protection. Peripheral blood level T-receptor excision rings (TREC) B-cell (KREC), respectively, can serve surrogate markers T- maturation. Currently, the diagnostic kits available on market have two significant disadvantages: i) are aimed at diagnosing immunodeficiency conditions only newborns...
The advent of direct-acting antiviral drugs (DAAs) was a breakthrough in the treatment patients with chronic hepatitis C, yet high viral replication errors can lead to development resistance associated variants (RAVs). Thus, assessment RAV infected is necessary monitor effectiveness. aim our study investigate presence primary mutations NS3 and NS5 regions HCV treatment-naive patients. Samples were taken from 42 who had not previously received DAA treatment. In present study, we used method...
Highly active antiretroviral therapy (HAART) is currently a combination of three (less frequently four) drugs; these target pathways involved in various stages HIV replication the body. Treatment failure problem facing doctors and patients using HAART. The most common cause therapeutic development drug resistance. emergence resistance associated with processes involving mutation occurring viral genome under influence evolutionary factors. Sequencing reactions were performed AmpliSens...
A method has been developed for HBV DNA finding in biological material at low viral load based on nested PCR with real-time detection of three targets. When developing the method, blood plasma samples were used from 128 CHB patients living regions Russian Federation and countries Central Asia 173 hemodialysis center North-West Federal District. Analytical sensitivity was tested using stepwise dilution method. detected by PCR. According to us, first stage, is amplified stage oligonucleotides...
Abstract To study the structure of human immunodeficiency virus (HIV)‐1 drug resistance (DR) in patients with newly diagnosed infection. Residents Republic Guinea ( N = 2168) were tested for HIV using enzyme‐linked immunosorbent assay (ELISA). Individuals a positive result further examined presence viral load blood plasma. was analyzed Sanger sequencing. The obtained sequences genotyped REGA (version 3.0) and MEGA 7. Analysis DR mutations performed Stanford University Database. Serological...
According to the latest data released by UNAIDS, global number of people living with HIV (PLHIV) in 2021 was 38.4 million, 1.5 million new infections. In different countries, a significant proportion these cases occur adult fertile population aged 15-49 years. Vietnam had national prevalence 0.3% total at end 2019, approximately 230,000 PLHIV. The most effective way prevent mother-to-child transmission is ART reduce maternal viral load. HIV-infected pregnant women should undergo monthly...
One of the most serious health problems in world are hepatotropic viruses that cause chronic liver disease. Hepatitis B virus is distributed globally; around 5% carriers also infected with hepatitis delta virus. Co-infection or superinfection and D significantly associated a much more severe disease, compared infection only However, examination for presence regions not mandatory. It should be noted complete genotype mapping isolated on territory CIS countries former Soviet Union, there yet,...
The Vietnam Ministry of Health (MOH) has intensified efforts in its aim to eliminate AIDS by 2030. Expanding the program for prevention mother-to-child transmission (PMTCT) is a significant step towards achieving this goal. However, there are still HIV-exposed children who do not have access PMTCT services, and some participated but contracted HIV. This study focused on assessing prevalence profile HIV mutations among under 18 months age had recently tested positive HIV, while gaining...
The aim of our work was to compare the HBV, HCV and HIV vertical transmission risk in Republic Guinea. Materials methods . material for study 305 blood plasma samples from pregnant women living Conakry, were examined presence serological (HBsAg, antibodies antiHBs IgG, anti-HBcore anti-HCV Ag/Ab-HIV) molecular (HBV DNA, RNA, RNA) markers. Results discussion When assessing overall prevalence markers among patients, incidence HBV 76.06 %. Antibodies detected only 1 case, which amounted 0.32 3...
The disease caused by the highly contagious SARS-CoV-2 virus – “novel coronavirus infection (COVID-19)” had killed more than 6.5 million people at end of December 2022. severity manifestation infectious process varies from asymptomatic forms to rapid progression life-threatening conditions requiring emergency measures. One factors, which affects outcome disease, is lymphopenia, cause may be a violation lymphopoiesis. identification laboratory markers high risk mortality in patients with...
According to the WHO data, number of infected people exceeded 765.2 million during COVID-19 pandemic. The severity patient’s condition is determined by immune system hyperactivation. Activation T- and B-lymphocyte subsets plays a prominent role in control infectious process. A content small circular DNA molecules — T-cell receptor excision circles (TREC circles) B-cell (“kappa”) rings (KREC Kappa-deleting recombination peripheral blood can be used as marker functionally active T B cells...
The aim of the study was to assess occurrence serological and molecular genetic markers HIV infection, viral hepatitis B C among persons in pre-trial detention. Materials methods. material identified 138 samples blood sequences obtained from men pretrial detention center St. Petersburg. Patients were examined by ELISA for presence infection (Ag/Ab HIV), HBV (HBsAg, antiHBs IgG, anti-HBcore IgG) HCV (anti-HCV IgG). Determination RNA, as well DNA PCR with hybridization-fluorescence detection...
Introduction. The spread of the human immunodeficiency virus type 1 (HIV-1) has become a global concern and approached pandemic status. St. Petersburg, major transportation, tourist, cultural, industrial center, border city, is characterized by high migration population. growing number migrants can contribute to importation new genetic variants trigger recombination processes in population Petersburg Leningrad Region.The aim characterize present-day HIV-1 subtype-specific profile...