ADVERTISEMENT RETURN TO ISSUEEditorialNEXTGreen Chemistry: A Framework for a Sustainable FutureKrishna N. GaneshKrishna GaneshMore by Krishna Ganeshhttps://orcid.org/0000-0003-2292-643X, Deqing ZhangDeqing ZhangMore Zhanghttps://orcid.org/0000-0002-5709-6088, Scott J. MillerScott MillerMore Millerhttps://orcid.org/0000-0001-7817-1318, Kai RossenKai RossenMore Rossenhttps://orcid.org/0000-0002-3857-0004, Paul ChirikPaul ChirikMore Chirikhttps://orcid.org/0000-0001-8473-2898, Marisa C....

Isothermal titration calorimetry (ITC) is a powerful and highly sensitive technique commonly used to study interactions between biomolecules in dilute aqueous solutions, both from thermodynamic kinetics points of view. In this report, we show that ITC may be follow the binding ligands such as amino acids surface inorganic materials gold nanoparticles. More specifically, have studied one basic acid, lysine, an acidic aspartic with nanoparticles at physiological pH. Strong acid under these...

Since its discovery 12 years ago, aminoethylglycyl peptide nucleic acid (aeg-PNA) has emerged as one of the successful DNA mimics for potential therapeutic and diagnostic applications. An important requisite in vivo applications that received inadequate attention is engineering PNA analogues able discrimination between RNA binding targets. Our approach toward this aim based on structural preorganization backbone to hybridization-competent conformations impart selectivity. This strategy...

The organization of nanoparticles into superstructures predefined geometry is an important challenge in the area nanoscale architecture. Attractive Coulombic interaction between positively charged amine groups on gold particle surfaces and negatively phosphate backbones DNA molecules (see Figure) drives self-assembly linear supercluster structures.

An isothermal titration calorimetric (ITC) investigation of the interaction DNA bases and PNA base monomers with gold nanoparticles is described revealing a binding sequence in order C > G A T. Direct measurement strength ligands nanogold by ITC has important implications surface modification strategies for biomedical, catalysis, nanoarchitecture applications.

Peptide Nucleic Acids (PNA) invented ten years ago are emerging rapidly as useful molecules in DNA hybridization and antisense techniques. Although therapeutic drugs based on PNA still not sight, applications diagnostics, particular situ PCR systems, making rapid strides. The simple structure of combination with impressive mimicking properties has already provided inspiration to many synthetic organic chemists for designing preparing several derivatives analogs towards improving their...

Inherently chiral, cationic am-PNAs having pendant aminomethylene groups at α(R/S) or γ(S) sites on PNA backbone have been synthesized. The modified PNAs are shown to stabilize duplexes with complementary cDNA in a regio- and stereo-preferred manner γ(S)-am superior α(R/S)-am α(R)-am better than the α(S) isomer. enhanced stabilization of am-PNA:DNA is accompanied by greater discrimination mismatched bases. This seems be combined result both electrostatic interactions conformational...

The assembly of nanoparticles in topologically predefined superstructures is an important problem the area nanoscale architecture. In this letter, we demonstrate electrostatic lysine-capped colloidal gold particles on drop-coated DNA films. Electrostatic interaction between positive charges and negative phosphate groups template molecules leads to linear superstructures. use as templates for shows promise extension more complex geometries through rational design base sequences well...

The major and minor groove in duplex DNA are sites of specific molecular recognition by DNA-binding agents such as proteins, drugs metal complexes have functional significance. In view this, understanding the inherent differences their environment allosteric information transfer between them induced assumes importance. Site-specific incorporation 5-aminodansyl-dU, (U * ) oligonucleotides d(CGCGAAU TCGCG) d(CGCGAATU CGCG) leads to fluorogenic nucleic acids, which reporter group resides...

Hybrid-peptides containing diphenylalanine and nucleoside were studied to understand the effects of C-terminus substituents, hydrophobicity H-bonding groups on self-assembly.

ADVERTISEMENT RETURN TO ISSUECommentNEXTGreen Chemistry: A Framework for a Sustainable FutureKrishna N. GaneshKrishna GaneshMore by Krishna Ganeshhttps://orcid.org/0000-0003-2292-643X, Deqing ZhangDeqing ZhangMore Zhanghttps://orcid.org/0000-0002-5709-6088, Scott J. MillerScott MillerMore Millerhttps://orcid.org/0000-0001-7817-1318, Kai RossenKai RossenMore Rossenhttps://orcid.org/0000-0002-3857-0004, Paul ChirikPaul ChirikMore Chirikhttps://orcid.org/0000-0001-8473-2898, Marisa C....

[formula: see text] The replacement of the glycyl component in peptide nucleic acid (PNA) backbone by a prolyl unit bearing nucleobase leads to aminoethylprolyl (aep) PNAs, which are chiral and cationic. homooligomeric aepPNA binds complementary DNA sequences with high affinity sequence specificity, forming highly stable triplexes.

[see structure]. Trinuclear MAS NMR, involving naturally abundant (13C, 15N) and easily deuterated (2H) nuclei, is shown to offer newer opportunities study molecular self-assembly in noncrystalline supramolecular systems.

To better understand DNA recognition and transcription activity by SATB1, the T-lineage-enriched chromatin organizer factor, we have determined its optimal DNA-binding sequence random oligonucleotide selection. The consensus SATB1-binding (CSBS) comprises a palindromic in which two identical AT-rich half-sites are arranged as inverted repeats flanking central cytosine or guanine. Strikingly, CSBS half-site is to conserved element 'TAATA' bound known homeodomains (HDs). Furthermore, show that...

PNAs grafted with cationic aminomethylene (am) pendants on the backbone at glycyl (α) or ethylenediamine (γ) segments show regio (α/γ) and stereochemistry (R/S) dependent binding complementary DNA. These are efficiently taken up by cells, γ(S-am)aeg-PNA being best in all properties.

Intrinsically cationic and chiral Cγ-substituted peptide nucleic acid (PNA) analogues have been synthesized in the form of γ(S)-ethyleneamino (eam)- γ(S)-ethyleneguanidino (egd)-PNA with two carbon spacers from backbone. The relative stabilization (ΔTm) duplexes modified PNAs as compared to 2-aminoethylglycyl (aeg)-PNA is better complementary DNA (PNA:DNA) than RNA (PNA:RNA). Inherently, PNA:RNA higher stability PNA:DNA duplexes, guanidino are superior amino PNAs. were found be specific...

Bimodal PNAs are new PNA constructs designed to bind two different cDNA sequences synchronously form double duplexes. They synthesized on solid phase using sequential coupling and click reaction introduce a second base in each monomer at Cα via alkyltriazole linker. The ternary bimodal PNA:DNA complexes show stability higher than that of individual appropriate create higher-order fused nucleic acid assemblies.

Peptide nucleic acids (PNAs) are linear equivalents of DNA with a neutral acyclic polyamide backbone that has nucleobases attached via tert-amide link on repeating units aminoethylglycine. They bind complementary or RNA sequence specificity to form hybrids more stable than the corresponding DNA/RNA self-duplexes. A new type PNA termed bimodal [Cγ(S/R)-bm-PNA] is designed have second nucleobase amide spacer side chain at Cγ aeg oligomer. Cγ-bimodal oligomers two per unit demonstrated...

ADVERTISEMENT RETURN TO ISSUEEditor's PageNEXTGreen Chemistry: A Framework for a Sustainable FutureKrishna N. GaneshKrishna GaneshMore by Krishna Ganeshhttps://orcid.org/0000-0003-2292-643X, Deqing ZhangDeqing ZhangMore Zhanghttps://orcid.org/0000-0002-5709-6088, Scott J. MillerScott MillerMore Millerhttps://orcid.org/0000-0001-7817-1318, Kai RossenKai RossenMore Rossenhttps://orcid.org/0000-0002-3857-0004, Paul ChirikPaul ChirikMore Chirikhttps://orcid.org/0000-0001-8473-2898, Marisa C....

Three-way junctions are simple and fundamental structural motifs that impart the typical branching property in most DNA nanostructures. While conventional three-way well-known, mesojunctions relatively unexplored. Here, we report synthesis of peptide nucleic acid (PNA)/DNA hybrid mesojunctions, containing a 14 bp duplex arm two PNA/DNA arms. We show mesojunction can be assembled magnesium-free buffers low concentrations calcium or sodium salts. showed higher thermal stability compared to...