A5009938266- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Molecular Sensors and Ion Detection
- DNA Repair Mechanisms
- Luminescence and Fluorescent Materials
- DNA and Nucleic Acid Chemistry
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Click Chemistry and Applications
- Advanced NMR Techniques and Applications
- Sulfur Compounds in Biology
- Chemical Synthesis and Analysis
- Cancer, Lipids, and Metabolism
- Crystallography and molecular interactions
- Cancer, Stress, Anesthesia, and Immune Response
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Organic Light-Emitting Diodes Research
- Genetic Neurodegenerative Diseases
- Cell death mechanisms and regulation
- Ion channel regulation and function
- Histone Deacetylase Inhibitors Research
- Concrete Corrosion and Durability
- MicroRNA in disease regulation
- Cancer therapeutics and mechanisms
Indian Institute of Science Education and Research Pune
2014-2025
Savitribai Phule Pune University
2015-2020
Tufts University
2004-2018
Indian Institute of Science Bangalore
2014
Massachusetts General Hospital
2011
Harvard University
2011
University of Wolverhampton
2004
Despite the great interest in artificial ion channel design, only a small number of channel-forming molecules are currently available for addressing challenging problems, particularly biological systems. Recent advances chloride-mediated cell death, aided by synthetic carriers, encouraged us to develop chloride selective supramolecular channels. The present work describes vicinal diols, tethered rigid 1,3-diethynylbenzene core, as pivotal moieties barrel-rosette formation, and activity such...
Transmembrane anion transport modality is enjoying a renewed interest because of recent advances toward anticancer therapy. Here we show bis(sulfonamides) as efficient receptors for selective Cl(-) ion binding and across lipid bilayer membranes. Anion-binding studies by (1)H NMR indicate logical correlation between the acidity sulfonamide N-H proton strength. Such recognition influenced further lipophilicity receptor during ion-transport process. The anion-binding activity bis(sulfonamide)...
Effective targeting of mitochondria has emerged as an alternative strategy in cancer chemotherapy. However, considering mitochondria's crucial role cellular energetics, metabolism and signaling, with small molecules would lead to severe side effects patients. Moreover, mitochondrial functions are highly dependent on other organelles like nucleus. Hence, simultaneous nucleus could more effective anticancer strategy. To achieve this goal, we have developed sub 200 nm particles from dual drug...
Abstract Cancer cells use elevated glutathione (GSH) levels as an inner line of defense to evade apoptosis and develop drug resistance. In this study, we describe a novel 2,4‐nitrobenzenesulfonyl (DNS) protected 2‐hydroxyisophthalamide system that exploits GSH for its activation into free forming supramolecular M + /Cl − channels. Better permeation the DNS compound MCF‐7 compared GSH‐activatable ion transport resulted in higher cytotoxicity, which was associated with increased oxidative...
A BODIPY-based selective thiophenol probe capable of discriminating aliphatic thiols is reported. The fluorescence off–on effect upon reaction with thiol elucidated theoretical calculations. sensing associated a color change from red to yellow and 63-fold enhancement in green fluorescence. Application the for detection demonstrated by live cell imaging.
Artificial biomimetic chloride anionophores have shown promising applications as anticancer scaffolds. Importantly, stimuli-responsive transporters that can be selectively activated inside the cancer cells to avoid undesired toxicity normal, healthy are very rare. Particularly, light-responsive systems promise better applicability for photodynamic therapy because of their spatiotemporal controllability, low toxicity, and high tunability. Here, in this work, we report o-nitrobenzyl-linked,...
A BODIPY-based fluorescence turn-on probe for lysosomal localization and biothiol sensing is reported.
We report a 2-hydroxy-<italic>N</italic><sup>1</sup>,<italic>N</italic><sup>3</sup>-diarylisophthalamide-based self-assembled channel that cotransports M<sup>+</sup>/Cl<sup>−</sup> and induces apoptotic cell death.
This research article explores the distinct TADF efficiency of three donor-acceptor based regio-isomers: DPAOCN (ortho-isomer), DPAMCN (meta-isomer), and DPAPCN (para-isomer). exhibits maximum in both solution solid-state with an impressive reverse inter-system crossing (RISC) rate ∼106 s-1; underlying cause being minimum singlet-triplet splitting energy or ΔEST SOC (spin-orbit coupling) between S1 & T1 states. Apart from TADF, differences crystal packing regio-isomers result intriguing bulk...
Fork stabilization at DNA impediments is key to maintaining replication fork integrity and preventing chromosome breaks. Mrc1 Tof1 are two known stabilizers that travel with the fork. In addition a structural role, has damage checkpoint function. Using yeast model system, we analyzed role of expanded CAG repeats medium long lengths, which stall forks cause trinucleotide expansion diseases such as Huntington's disease myotonic dystrophy. We demonstrate stabilizer but not activation function...
Intrinsically cationic and chiral Cγ-substituted peptide nucleic acid (PNA) analogues have been synthesized in the form of γ(S)-ethyleneamino (eam)- γ(S)-ethyleneguanidino (egd)-PNA with two carbon spacers from backbone. The relative stabilization (ΔTm) duplexes modified PNAs as compared to 2-aminoethylglycyl (aeg)-PNA is better complementary DNA (PNA:DNA) than RNA (PNA:RNA). Inherently, PNA:RNA higher stability PNA:DNA duplexes, guanidino are superior amino PNAs. were found be specific...
Synthetic chloride carriers are known to induce chloride-mediated apoptosis inside cancer cells. One of the main disadvantages is unfavorable cytotoxicity towards healthy cells due lack selectivity. The use stimuli, such as light, enzymes, ligands, etc., has enabled selective activation these systems in Light, notably, a significant stimulus that been utilized its excellent spatiotemporal control, remote addressability, and low cytotoxicity. However, previously reported photoresponsive...
Expansion of trinucleotide repeat sequences is the cause multiple inherited human genetic diseases including Huntington's disease and myotonic dystrophy. CTG CAG repeats have been shown to form stable secondary structures that can impair Okazaki fragment processing may impede replication fork progression. We recently showed mutation DNA damage checkpoint proteins results in increased chromosome breaks at expanded CAG/CTG instability (expansions contractions).(1) Here we report long...
Although cocaine- and amphetamine-regulated transcript peptide (CART) is detected in several cortical subcortical areas, its role higher functions has been largely ignored. We examined the significance of CART memory formation tested if downstream actions involve N-methyl-d-aspartate (NMDA) activated extra-cellular signal-regulated kinase (ERK). Newly formed was evaluated using novel object recognition test consisting familiarization (T1) choice trials (T2). The were performed at two time...
Multifunctional organic luminogens exhibiting simultaneous aggregation induced emission (AIE), room-temperature phosphorescence (RTP), and mechanochromism have recently attracted considerable attention owing to their potential applications in optoelectronics bioimaging. However, a comprehensive correlation among these three distinguished properties is yet be unveiled, which will help decipher defined methodologies design future generation multifunctional materials. Herein, we demonstrated...
The DNA damage checkpoint signalling cascade sense damaged and coordinates cell cycle arrest, repair, and/or apoptosis. However, it is still not well understood how the system differentiates between different kinds of damage. N-nitroso-N-ethylurea (NEU), a ethylating agent induces both transversions transition mutations. Immunoblot comet assays were performed to detect breaks activation canonical kinases following NEU upto 2 hours. To investigate whether mismatch repair played role in...
Abstract The formation of a supramolecular synthetic M + /Cl − channel in the membrane phospholipid bilayer has been reported upon activation methyl pivalate‐linked N 1 , 3 ‐dialkyl‐2‐hydroxyisophthalamide by esterases. induces apoptosis cancer cells via intrinsic pathway. Interestingly, was also shown to disrupt autophagy causing alkalization lysosomes – feature that confirmed at cellular and protein level.
Abstract Cancer cells use elevated glutathione (GSH) levels as an inner line of defense to evade apoptosis and develop drug resistance. In this study, we describe a novel 2,4‐nitrobenzenesulfonyl (DNS) protected 2‐hydroxyisophthalamide system that exploits GSH for its activation into free forming supramolecular M + /Cl − channels. Better permeation the DNS compound MCF‐7 compared GSH‐activatable ion transport resulted in higher cytotoxicity, which was associated with increased oxidative...