A5035269942- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Carcinogens and Genotoxicity Assessment
- Nuclear Structure and Function
- Cancer-related Molecular Pathways
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
Ludwig Cancer Research
2022-2023
University of California, San Diego
2022
University of Virginia
2015-2020
Indian Institute of Science Education and Research Pune
2014
University of Virginia Medical Center
2014
Predicting the response and identifying additional targets that will improve efficacy of chemotherapy is a major goal in cancer research. Through large-scale vivo vitro CRISPR knockout screens pancreatic ductal adenocarcinoma cells, we identified genes whose genetic deletion or pharmacologic inhibition synergistically increase cytotoxicity MEK signaling inhibitors. Furthermore, show viability scores combined with basal gene expression levels could model global cellular responses to drug...
Abstract Proper chromosome segregation depends upon kinetochore phosphorylation by the Chromosome Passenger Complex (CPC). Current models suggest activity of CPC decreases in response to inter-kinetochore stretch that accompanies formation bi-oriented microtubule attachments, however little is known about tension-independent phosphoregulation. Microtubule bundles initially lie close proximity inner centromeres and become depleted metaphase. Here we find these microtubules control a tension...
Centromeric chromatin is required for kinetochore assembly during mitosis and accurate chromosome segregation. A unique nucleosome containing the histone H3–specific variant CENP-A defining feature of centromeric chromatin. In humans, deposition occurs in early G1 just after mitotic exit at time when machinery localizes to centromeres. The mechanism by which deposited onto an existing, condensed template not understood. Here we identify selective association chaperone HJURP with condensin II...
Functional characterisation of proteins and large-scale, systems-level studies are enabled by extensive sets cloned open reading frames (ORFs) in an easily-accessible format that enables many different applications. Here we report the release first stage Xenopus ORFeome, which contains 8673 ORFs from Gene Collection (XGC) for laevis, into a Gateway® donor vector enabling rapid in-frame transfer to expression vectors. This resource represents estimated 7871 unique genes, approximately 40%...
The DNA damage checkpoint signalling cascade sense damaged and coordinates cell cycle arrest, repair, and/or apoptosis. However, it is still not well understood how the system differentiates between different kinds of damage. N-nitroso-N-ethylurea (NEU), a ethylating agent induces both transversions transition mutations. Immunoblot comet assays were performed to detect breaks activation canonical kinases following NEU upto 2 hours. To investigate whether mismatch repair played role in...
Summary Chromothripsis, the shattering and imperfect reassembly of one (or a few) chromosome(s) 1 , is an ubiquitous 2 mutational process generating localized complex chromosomal rearrangements that drive genome evolution in cancer. Chromothripsis can be initiated by missegregation errors mitosis 3,4 or DNA metabolism 5-7 lead to entrapment chromosomes within micronuclei their subsequent fragmentation next interphase upon mitotic entry 6,8-10 . Here, we use inducible degrons demonstrate...