A5074565889- Milk Quality and Mastitis in Dairy Cows
- Vitamin D Research Studies
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Infant Nutrition and Health
- Advanced Proteomics Techniques and Applications
- Probiotics and Fermented Foods
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- Reproductive Physiology in Livestock
- Bacteriophages and microbial interactions
- T-cell and B-cell Immunology
- Animal Genetics and Reproduction
- Identification and Quantification in Food
- Cancer-related molecular mechanisms research
- Reproductive System and Pregnancy
- MicroRNA in disease regulation
- Leptospirosis research and findings
- Metabolomics and Mass Spectrometry Studies
- Mycobacterium research and diagnosis
- Immune Response and Inflammation
- Microbial infections and disease research
- Mass Spectrometry Techniques and Applications
- Digestive system and related health
National Animal Disease Center
2016-2025
Agricultural Research Service
2016-2025
United States Department of Agriculture
2014-2025
Ames National Laboratory
2019
Teagasc - The Irish Agriculture and Food Development Authority
2014
New York State College of Veterinary Medicine
2014
Cornell University
2014
Carter Center
2000-2002
University of Virginia
2000-2002
University of Washington
2001
Milk fat globule membranes (MFGM) were isolated from the milk of mid-lactation Holstein cows. The purified MFGM fractionated using 1-dimensional SDS gels. Tryptic peptides gel slices further on a micro-capillary high performance liquid chromatograph connected to nanospray-tandem mass spectrometer. Analysis data resulted in 120 proteins being identified by two or more unique peptide sequences. Of these proteins, 71% are membrane associated with remainder cytoplasmic secreted proteins. Only 15...
The means by which calcium is transported into the milk produced mammary glands a poorly understood process. One hypothesis that it occurs during exocytosis of secretory products via Golgi pathway, consistent with observation SPCA1 Ca2+-ATPase, expressed in Golgi, induced lactating tissue. However, massive up-regulation PMCA2bw plasma membrane Ca2+-ATPase also lactation and more strongly correlated increases calcium, suggesting may be secreted directly this pump. To examine physiological...
Deficiency of serum levels 25-hydroxyvitamin D3 has been related to increased risk lower respiratory tract infections in children. Respiratory syncytial virus (RSV) is a leading cause low infants and young The neonatal calf model RSV infection shares many features common with In the present study, we hypothesized that calves circulating (25(OH)D3) would be more susceptible than high 25(OH)D3. Calves were fed milk replacer diets different vitamin D for 10 wk period establish two treatment...
Numerous in vitro studies have shown that toll-like receptor signaling induces 25-hydroxyvitamin D3 1α-hydroxylase (1α-OHase; CYP27B1) expression macrophages from various species. 1α-OHase is the primary enzyme converts to 1,25-dihydroxyvitamin (1,25(OH)2D3). Subsequently, synthesis of 1,25(OH)2D3 by has been modulate innate immune responses macrophages. Despite numerous induced macrophages, however, evidence pathogens vivo limited. The objective this study was evaluate gene and mammary...
Bacterial biofilms are organized sessile communities of bacteria enclosed in extracellular polymeric substances (EPS). To analyze organization and EPS high resolution magnification by scanning electron microscopy (SEM), it is important to preserve the complex architecture biofilms. Therefore, fixation abilities formalin, glutaraldehyde, Methacarn (methanol/chloroform/acetic acid-6:3:1) fixatives were evaluated identify which fixative would best structure bacterial Economically Gram-negative...
T cell recognition of autoantigens is critical to progressive immune-mediated destruction islet cells, which leads autoimmune diabetes. We identified a naturally presented autoantigen from the human antigen glutamic acid decarboxylase, 65-kDa isoform (GAD65), by using combination chromatography and mass spectrometry peptides bound type I diabetes (insulin-dependent mellitus, IDDM)-associated HLA-DR4 molecule. Peptides encompassing this epitope-stimulated GAD65-specific cells diabetic...
Abstract Peptides associated with class II MHC molecules are of variable length because in contrast to peptides I molecules, their amino and C termini not constrained by the structure peptide interaction binding site. The proteolytic processing events that generate these still well understood. To address this question, extracted from HLA-DR*0401 were analyzed using two types mass spectrometry instrumentation. This enabled identification >700 candidate a single analysis provided...
Simian virus 40 large tumor (T) antigen contains three H-2Db-restricted (I, II/III, and V) one H-2Kb-restricted (IV) cytotoxic T lymphocyte (CTL) epitopes. We demonstrate that a hierarchy exists among these CTL epitopes, since vigorous responses against epitopes I, IV are detected following immunization of H-2b mice with syngeneic, T-antigen-expressing cells. By contrast, weak response the epitope V was only loss variant B6/K-3,1,4 cells, which have lost expression IV. Limiting-dilution...
Deficiency of serum levels 25-hydroxyvitamin D3 has been correlated with increased risk infectious diseases such as tuberculosis and influenza. A plausible reason for this association is that expression genes encoding important antimicrobial proteins depends on concentrations 1,25-dihydroxyvitamin produced by activated immune cells at sites infection, synthesis dependent the availability D3. Thus, increasing 25(OH)D3 cell infection hypothesized to aid in clearance infection. This report...
γδ T cells respond to stimulation via toll-like receptors (TLR). Bovine express TLR3 and TLR7, that are key for the recognition of viruses such as bovine respiratory syncytial virus (BRSV); however, responses these receptors, their role during viral infections, remains unclear. Here, we demonstrate neonatal exhibit robust chemokine cytokine production in response agonist, Poly(I:C), TLR7 Imiquimod. Importantly, observe a similar phenotype T-cell subsets purified from calves infected with...