A5013546879- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Pluripotent Stem Cells Research
University of Buenos Aires
2018-2020
Abattoir ovaries, which are the main source of oocytes for reproductive biotechnologies, arrive at laboratory under ischaemic conditions. Reoxygenation generates reactive oxygen species (ROS) in tissues, could affect oocyte quality. The aim this study was to evaluate effect supplementation media with dimethylthiourea (DMTU) during collection and washing cumulus–oocyte complexes (COC) on ROS levels, COC apoptosis nuclear cytoplasmic maturation. Thus, (TCM-199) (TCM-199 10% porcine follicular...
Abstract The aim of this study was to assess the presence and distribution apoptosis in porcine cumulus‐oocyte complexes (COCs) its relations with COC morphology developmental competence. COCs were obtained from slaughterhouse ovaries, classified into A1 (top category), A2, B1, B2, C, D based on their morphology. A1, B1 matured fertilized vitro, blastocyst rate compared among them. Before after vitro maturation (IVM), annexin‐V staining, terminal deoxynucleotidyl transferase dUTP nick end...
The aim of this study was to evaluate the effect antioxidant dimethylthiourea (DMTU) as a supplement during collection and washing cumulus–oocyte complexes (COC) on apoptosis in cumulus cells (CC) competition for oocyte development vitro. COC were obtained by follicular aspiration. aspiration medium TCM-199 with 10% porcine fluid, 0.3 mM sodium pyruvate, penicillin/streptomycin. Treated groups supplemented 2 or 20 DMTU. After 44 h vitro maturation (IVM), percentage nuclear determined...