Long non-coding RNAs (lncRNAs) have been defined as transcripts longer than 200 nucleotides, which lack significant protein coding potential and possess critical roles in diverse cellular processes. recently functionally characterized plant stress-response mechanisms. In the present study, we perform a comprehensive identification of lncRNAs response to combined stress induced by salinity excess boron Lluteño maize, tolerant maize landrace from Atacama Desert, Chile. We use deep RNA...

Chromatin-remodeling factors regulate the establishment of transcriptional programs during plant development. Although 42 genes encoding members SWI2/SNF2 family have been identified in Arabidopsis thaliana , <10 assigned a precise function on basis mutant phenotype, and none shown to play specific role gametophytic phase life cycle. A. chromatin-remodeling protein 11 ( CHR11 ) encodes an imitation switch (ISWI)-like abundantly expressed female gametogenesis embryogenesis . To determine...

In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from single larva or pupa.This efficiently obtains small specimens, which is difficult and challenging because amount starting tissue.Maceration liquid nitrogen, phenol treatment, ethanol precipitation step are eliminated using methodology.The A 260 /A 280 absorbance ratios isolated were approximately 1.8, suggesting that pure can be used further...

Eugnosta azapaensis Vargas and Moreira, 2015 (Lepidoptera: Tortricidae) is a micromoth native to the Atacama Desert whose larvae induce fusiform galls in shoots of Baccharis salicifolia (Ruiz & Pav.) Pers. (Asteraceae). The presence this cecidogenous tortricid was previously recorded only from type locality, Azapa Valley, Arica Province, northern Chile. However, on B. were recently found Chaca, another coastal valley Desert. adults obtained these preliminarily identified as E. based...

Prey identification in nests of the potter wasp Hypodynerus andeus (Packard) (Hymenoptera, Vespidae, Eumeninae) using DNA barcodes. Geometrid larvae are only prey known for Neotropical (Packard, 1869) coastal valleys northern Chilean Atacama Desert. A fragment mitochondrial gene cytochrome oxidase c subunit 1 was amplified from geometrid collected cells H. Azapa Valley, Arica Province, and used to provide taxonomic identifications. Two species, Iridopsis hausmanni Vargas, 2007 Macaria...

The Region of Arica and Parinacota (Atacama Desert) offers several unexplored remote sites with unique characteristics that would allow for the formulation new bioproducts agriculture. Among them, Jurasi Hot Springs, Polloquere Amuyo Lagoons represent a group open pools fed by thermal water springing from mountains. Their microbiomes remain unspecified, providing opportunity to characterize endemic community these develop sustainable Bacteria were isolated sediments geothermal characterized...

Oregano from Socoroma (Atacama Desert) is characterized by its unique organoleptic properties and distinctive flavor it produced using ancestral pesticide-free agricultural practices performed the Aymara communities. The cultivation in this zone carried out under extreme conditions where standard production of different crops limited several environmental factors, including aridity, high concentration salts, boron among others. However, oregano plants are associated with microorganisms that...

Late embryogenesis abundant (LEA) proteins are a large protein family that mainly function in protecting cells from abiotic stress, but these also involved regulating plant growth and development. In this study, we performed functional analysis of LEA13 LEA30 Arabidopsis thaliana. The results showed the expression both genes increased when plants were subjected to drought-stressed conditions. insertional lines lea13 lea30 identified for each gene, had T-DNA element regulatory region, which...

Onion (Allium cepa L) is an important vegetable crop in Chile with 3.675 ha (ODEPA, www.odepa.gob.cl). Since 2017, a series of symptoms has been increasingly observed, mainly mature bulbs which include water-soaked lesions on leaves and external central cataphylls, evolve to necrotic rot, reducing yields affecting the postharvest life. As season progresses over 90% incidence estimated severely affected fields. Symptomatic plants were collected for disease identification summer (February...

To understand the molecular stress response in maize plants to high salt and boron (B) stress, we focused on transcript accumulation of six stress-related genes Lluteño maize, a sweet corn landrace from Lluta valley (northern Chile).This is tolerant B stress.A randomized complete block design with four replications was used.Seedlings hybrid B73 were exposed 150 mM NaCl 20 ppm nutrient solution for 120 hrs, then root leaf samples collected Na + content determined.Transcript three SOS1, NHX2...

is an endemic plant species of the Atacama Desert, with unique morphological and physiological adaptations. This research was conducted at Cuesta El Águila, Arica Parinacota Region, Chile. Adult juvenile plants were monitored, recording their growth, flowering, fruiting phases. Additionally, community identified. For study endophytic mycoflora, samples seeds, roots, stems, leaves collected, disinfected, cultivated in specific media. The isolated fungi analyzed morphologically molecularly,...

Abstract The adult, pupa, larva and biology of Adenogasteria leguminivora Davis & Vargas gen. et sp. nov. (Lepidoptera: Gracillariidae: Ornixolinae) from Peru Chile are described illustrated. This micromoth was previously reported in the agronomic literature as a pest Caesalpinia spinosa (Molina) (Fabaceae) Peru, but its taxonomic status remained unknown until now. Its larvae feed on seeds three legume trees: Acacia macracantha , C. Inga feuillei . superficially resembles other sympatric...

Abstract Although considered the driest desert in world, Atacama provides unique habitats and a reservoir of biodiversity for plants insects. Caloptilia Hübner, 1825 (Gracillariinae), is highly diverse genus leaf miner micromoths that has not yet been recorded Chile. In this study, we describe new species from relict, vulnerable ecotone transverse valleys Desert northern Adults, immature stages natural history, including mine guacanivora Vargas‐Ortiz & Vargas sp. nov., are described...

To investigate the genetic and molecular regulation that female gametophyte could exert over neighboring sporophytic regions of ovule, we performed a quantitative comparison global expression in wild-type nozzle/sporocyteless (spl) ovules Arabidopsis thaliana (Arabidopsis), using Massively Parallel Signature Sequencing (MPSS). This resulted 1517 genes showing at least 3-fold increased lacking gametophyte, including those encoding 89 transcription factors, 50 kinases, 25 proteins containing...

Extraction of high quality DNA from fungus can be affected because the presence complex cell wall, content polysaccharides or secondary metabolites.In this research, we adapt a simple method extraction and purification isolated soil for Polymerase Chain Reaction (PCR) applications.The methodology described is both rapid cost effective.

Presence of false smut (Graphiola phoenicis (Moug.ex Fr.) Poit.) on Canary date palm (Phoenix canariensis) Easter Island, Chile.Cien.Inv.Agr.44(3): 307-311.Graphiola was found leaves Phoenix canariensis in Hanga Roa, Chile.Amphigenous black pulvinate basidiomata were abundant the leaf blades and rachides, causing extensive foliar damage.The samples examined by a light microscope after three days humid chamber.In addition, molecular tools scanning electron microscopy histological sectioning...

Tillandsia marconae Till & Vitek (Bromeliaceae) is a rare plant native species that grows over sand, in the coastal desert from Perú and Chile considered an endangered species. In this study, we assembled its chloroplast genome. The draft genome of T. ca. 158,873 bp length, containing large single-copy region 86,937 bp, small 18,506 pair inverted repeat regions 26,715 bp. GC content 37.4%. It encodes total 135 genes, including 86 protein-coding 38 tRNA 8 rRNA three pseudogenes. phylogenetic...

Host-associated larval polychromatism is described for the first time Neotropical hairstreak Strymon bubastus (Stoll, 1780) (Lepidoptera, Lycaenidae, Theclinae, Eumaeini) based on larvae collected in Atacama Desert of northern Chile inflorescences Phyla nodiflora (L.) Greene (Verbenaceae) and Waltheria ovata Cav. (Malvaceae) reared to adult. This record a host plant family Verbenaceae S. bubastus. Although other Malvaceae already have been recorded as its hosts, this it feeding W. ovata....

Tillandsia landbeckii Phil. is a vulnerable species belonging to the terrestrial Bromeliaceae family; it highly adapted extremely hyper-arid conditions of Atacama Desert and Peruvian deserts. In this study, we sequenced, assembled, annotated its chloroplast genome. T. genome 159,131 bp in length, containing large single-copy region 87,164 bp, small 18,521 pair inverted repeat regions 26,723 bp. The GC content 37.33%. It encodes total 132 genes, including 86 protein-coding 38 tRNA genes 8...

The little-known Alucita danunciae Vargas, 2011 (Lepidoptera: Alucitidae) is recorded for the first time from Peru, expanding its altitudinal, geographic and host plant ranges. Its larvae were found on fruits of Tecoma fulva arequipensis (Bignoniaceae) at 2900 m elevation in Pocsi, Arequipa, western slopes Andes southern Peru. Genetic divergence between Chilean (type locality) Peruvian DNA barcode haplotypes was 0.6–0.9% K2P.