The rhamnolipids produced by Pseudomonas aeruginosa strains are often a mixture of several homologues. Up to seven (R2C10C10 + R1C10C10 R2C10C12 R1C10C12 R1C12:1C10 R1C12:2 R1C8:2 ) have been identified in cultures P. AT10 from soybean oil refinery wastes. This study deals with the production, purification, and identification (M7), as well their physical chemical characterization evaluation antimicrobial properties. surface tension decreases 26.8 mN/m critical micelle concentration value 1.2...

Two series of long chain imidazolium- and pyridinium-based ionic liquids containing an ester functional group in the alkyl side chain, 3-methyl-1-alkyloxycarbonylmethylimidazolium bromides (CnEMeImBr) 1-alkyloxycarbonylmethylpyridinium (CnEPyrBr), were synthesized their thermal stability, aggregation behavior aqueous medium, antimicrobial activity investigated. The introduction decreased stability functionalized ILs compared to simple (1-alkyl-3-methylimidazolium 1-alkylpyridinium bromides)....

World production of oils and fats is about 2.5 million tonnes, 75% which are derived from plants. Most them used in the food industry for manufacture different products, or directly as salad oil. Great quantities waste generated by oil fat industries: residual oils, tallow, marine soap stock, frying oils. It well known that disposal wastes a growing problem new alternatives use fatty should be studied. Used due to their composition, have great potential microbial growth transformation. The...

Abstract Pseudomonas aeruginosa 47T2, grown in submerged culture with waste frying oil as a carbon source, produced mixture of rhamnolipids surface activity. Up to 11 rhamnolipid homologs (Rha‐Rha‐C 8 ‐C 10 ; Rha‐C /Rha‐C ;Rha‐Rha‐C 12:1 Rha‐Rha‐C 12 /Rha‐Rha‐C 14:1 ) were isolated from cultures P. 47T2 and identified by HPLC‐MS analysis. This article deals the production, isolation, chemical characterization RL . The physicochemical biological properties new product also studied. Its...

Aims: Here we study the effect of monohydrochloride l-arginine, Nα-lauroyl ethylester (LAE), a cationic preservative derived from lauric acid and arginine, on cell envelopes Salmonella typhimurium Staphylococcus aureus at sub-lethal concentration such as their respective minimal inhibitory concentrations, 32 8 μg ml−1, respectively. Methods Results: Bacterial populations were studied by using transmission electron fluorescence microscopy (TEM FM), flow cytometry (FC) ion-flux across cellular...

To study cellular damage induced by Cinnamomum verum essential oil in Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus 29213.The effect of cinnamon bark on these two strains was evaluated plate counts, potassium leakage, flow cytometry transmission electron microscopy (TEM). Exposure to this alterations the bacterial membrane Ps. aeruginosa, which led collapse potential, as demonstrated bis-oxonol staining, loss membrane-selective permeability, indicated efflux K(+) propidium...

Evaluation of the cellular effects Origanum compactum essential oil on Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus 29213.The damage induced by O. these two strains has been studied using different techniques: plate count, potassium leakage, flow cytometry (FC) transmission electron microscopy (TEM). The results showed that treatment led to reduction cells viability dissipated ion gradients. Flow cytometric analysis promoted accumulation bis-oxonol membrane-impermeable nucleic...

Three different sets of cationic surfactants from lysine have been synthesized. The first group consists three monocatenary with one as the polar head charge. second two amino acids positive charges. Finally, four gemini were synthesized in which spacer chain and number type charges regulated. micellization process, antimicrobial activity, hemolytic activity evaluated. critical micelle concentration was dependent only on hydrophobic character molecules. Nevertheless, activities related to...

The present work aims to investigate the antioxidant and antimicrobial activities as well potential of DCS1 lipopeptides produced by Bacillus methylotrophicus strain at inhibition disruption biofilm formation.The biosurfactants were characterized molecules using thin layer chromatography (TLC) Fourier transform infrared spectroscopy (FT-IR). assayed for their activity through five different tests. scavenging effect on DPPH radicals a concentration 1 mg mL-1 was 80.6%. reducing power reached...

Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis evaluate the application of in prevention disruption pathogenic micro-organism biofilm that creates health problems food industry hospitals.An containing molasses was optimized enhance production RSM. A 3·2 g l(-1) achieved with optimum 107·82 molasses, 6·47 NaNO3 9·7 K2 HPO4 /KH2 PO4 , phosphate salts had significant effect...

In this work the synthesis and evaluation of a novel class gemini biocompatible surfactants from arginine with chemical, biological, fundamental research applications are presented for first time. strategy environmental acceptability already single chain Nα-acyl was reinforced by chemical dimerization in order to obtain surfactant high physicochemical efficiency low toxicity. The application these molecules (with very intrinsic toxicity) industrial processess could lead dramatic reduction...

Abstract Microbes have been isolated from soil and water samples after an enrichment culture process with kerosene tested for biosurfactant production by measuring the surface interfacial tensions emulsification power of broths. The isolation characterization extracellular surface‐active agents broth Pseudomonas aeruginosa 44T1 strain made. Preliminary structure identification specific TLC reagents CHCl 3 MeOH (2:1) extracts showed two spots a glycolipidic Rf values 0.70 0.45, respectively,...