A5086961503- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Cytomegalovirus and herpesvirus research
- Urinary Tract Infections Management
- Infectious Diseases and Mycology
- Herpesvirus Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Orthopedic Infections and Treatments
- Neonatal and Maternal Infections
- Parasite Biology and Host Interactions
- Pneumonia and Respiratory Infections
- Pelvic floor disorders treatments
- Parasites and Host Interactions
- Mycobacterium research and diagnosis
- Antibiotics Pharmacokinetics and Efficacy
- Pediatric Urology and Nephrology Studies
- Plant Pathogens and Fungal Diseases
- Drug-Induced Adverse Reactions
- Renal Transplantation Outcomes and Treatments
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Complement system in diseases
- Helminth infection and control
Leiden University Medical Center
2016-2025
Loyola University Medical Center
2021
Czech Academy of Sciences, Institute of Microbiology
2012-2020
Radboud University Nijmegen
2013-2020
Radboud University Medical Center
2020
Canisius-Wilhelmina Ziekenhuis
2020
University Medical Center Groningen
2020
National Institute for Public Health and the Environment
2020
Amsterdam University Medical Centers
2020
Vrije Universiteit Amsterdam
2020
Triazole resistance is an increasing problem in invasive aspergillosis (IA). Small case series show mortality rates of 50%–100% patients infected with a triazole-resistant Aspergillus fumigatus, but direct comparison triazole-susceptible IA lacking. A 5-year retrospective cohort study (2011–2015) was conducted to compare voriconazole-susceptible and voriconazole-resistant IA. fumigatus culture-positive were investigated identify proven, probable, putative Clinical characteristics, day 42 90...
In patients with invasive aspergillosis (IA), fungal cultures are mostly negative. Consequently, azole resistance often remains undetected. The AsperGenius® multiplex real-time PCR assay identifies clinically relevant Aspergillus species and four resistance-associated mutations (RAMs; TR34/L98H/T289A/Y121F) in the Cyp51A gene. This multicentre study evaluated diagnostic performance of this on bronchoalveolar lavage (BAL) fluid correlated presence RAMs treatment failure mortality. Stored BAL...
This study investigated the dynamics of Aspergillus fumigatus azole-resistant phenotypes in two compost heaps with contrasting azole exposures: free and exposed. After heat shock, to which sexual but not asexual spores are highly resistant, azole-free yielded 98% (49/50) wild-type 2% (1/50) isolates, whereas azole-containing 9% (4/45) 91% (41/45) resistant isolates. From latter compost, 80% (36/45) isolates contained TR46/Y121F/T289A genotype, (1/45) harbored TR46/Y121F/M172I/T289A/G448S had...
Abstract Background Invasive aspergillosis (IA) by a triazole-resistant Aspergillus fumigatus is associated with high mortality. Real-time resistance detection will result in earlier initiation of appropriate therapy. Methods In prospective study, we evaluated the clinical value AsperGenius polymerase chain reaction (PCR) assay hematology patients from 12 centers. This PCR detects most frequent cyp51A mutations A. conferring azole resistance. Patients were included when computed tomography...
Pre-existing lower urinary tract symptoms (LUTS), cognitive impairment, and the high prevalence of asymptomatic bacteriuria (ASB) complicate diagnosis infection (UTI) in older women. The presence pyuria remains cornerstone UTI diagnosis. However, >90% ASB patients have pyuria, prompting unnecessary treatment. We quantified by automated microscopy flowcytometry to determine diagnostic accuracy for derive thresholds women.Women ≥65 years with ≥2 new-onset LUTS 1 uropathogen ≥104 colony-forming...
In adults with febrile urinary tract infection (fUTI), data on optimal treatment duration in patients other than non-pregnant women without comorbidities are lacking.A randomized placebo-controlled, double-blind, non-inferiority trial among 35 primary care centers and 7 emergency departments of regional hospitals the Netherlands. Women men aged ≥ 18 years a diagnosis fUTI were randomly assigned to receive antibiotic for or 14 days (the second week being ciprofloxacin 500 mg placebo orally...
Background Blood cultures are considered the gold standard to distinguish bacteremia from non-bacteremic systemic inflammation. In current clinical practice, bacteraemia is unlikely if blood have been negative for 48–72 hours. Modern BC systems reduced this time-to-positivity (TTP), questioning whether time frame of hrs still valid. This study investigates distribution TTP, probability culture positivity after 24 hours, and identifies predictors prolonged TTP. Methods Adult patients with...
Increasing resistance of Aspergillus fumigatus to triazoles in high-risk populations is a concern. Its impact on mortality not well understood, but rates from 50% 100% have been reported.To determine the prevalence voriconazole-resistant A. invasive aspergillosis (IA) and its associated large multicentre cohort haematology patients with culture-positive IA.We performed retrospective study, which outcomes proven/probable IA were analysed. Patients stratified based voriconazole susceptibility...
Abstract Background Postoperative ileus is a common complication of abdominal surgery, leading to patient discomfort, morbidity and prolonged postoperative length hospital stay (LOS). Previous studies suggested that chewing gum stimulates bowel function after but were underpowered evaluate its effect on LOS did not include enhanced recovery surgery (ERAS)-based perioperative care. This study evaluated whether elective reduces time in the setting ERAS-based Methods A multicentre RCT was...
We investigated the prevalence of azole resistance Aspergillus fumigatus isolates in Netherlands by screening clinical A. for during 2013-2018. analyzed azole-resistant phenotypically vitro susceptibility testing and presence mutations Cyp51A gene. Over 6-year period, 508 (11%) 4,496 culture-positive patients harbored an isolate. Resistance frequency increased from 7.6% (95% CI 5.9%-9.8%) 2013 (58/760 patients) to 14.7% 12.3%-17.4%) 2018 (112/764 (p = 0.0001). TR34/L98H (69%)...
COVID-19 associated pulmonary aspergillosis (CAPA) is with increased morbidity and mortality in ICU patients. We investigated the incidence of, risk factors for potential benefit of a pre-emptive screening strategy CAPA ICUs Netherlands/Belgium during immunosuppressive treatment.A retrospective, observational, multicentre study was performed from September 2020-April 2021 including patients admitted to who had undergone diagnostics CAPA. Patients were classified based on 2020 ECMM/ISHAM...
A high prevalence of COVID-19 associated pulmonary aspergillosis (CAPA) has been reported, though histopathological evidence is frequently lacking. To assess the clinical significance Aspergillus species in respiratory samples mechanically ventilated patients, we implemented routine screening for tracheal aspirate (TA).
Cytomegalovirus (CMV) infections after transplantation are commonly treated using a prophylactic or preemptive regimen with (val)ganciclovir. It remains unclear, which approach is most effective in preventing CMV disease D+R- patients. The aim of this retrospective study was to compare the treatment response and antiviral resistance between two regimens renal transplant recipients.Before 2006, valganciclovir applied (42 patients). From 2006 onwards, patients first received prophylaxis for 90...
Varicella zoster virus (VZV) infections are a relevant cause of morbidity and mortality in hematological patients especially hematopoietic stem cell transplant (HSCT) recipients. The present study aimed to investigate the prevalence clinical significance viral persistence antiviral resistance by systematically analyzing all episodes VZV diagnosed our laboratory pediatric adult between 2007 2010.Patient charts were reviewed document patient disease characteristics. loads determined available...
Journal Article Feasibility and Effects of a Diabetes Type II Protocol with Blood Glucose Self-Monitoring in General Practice Get access G RUTTEN, RUTTEN Correspondence to: G. Rutten, Haagstraat 19,6641 AH Beuningen, The Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar J VAN EIJK, EIJK *Department Nursing Home Mediane, Free University AmsterdamNijmegen, E DE NOBEL, NOBEL **Department Internal Medicine, HospitalNijmegen, M BEEK, BEEK H DER VELDEN...
A survey of diagnosis and treatment invasive aspergillosis was conducted in eight University Medical Centers (UMCs) non-academic teaching hospitals the Netherlands. Against a background emerging azole resistance Aspergillus fumigatus routine screening clinical isolates performed primarily UMCs. Azole rates at hospital level varied between 5% 10%, although up to 30% were reported high-risk wards. Voriconazole remained first choice for 13 out 16 hospitals. In documented 14 centres treated...
To accelerate the development of novel vaccines for schistosomiasis, we set out to develop a human model Schistosoma mansoni infection in healthy volunteers. During natural infections, female schistosomes produce eggs that give rise morbidity. Therefore, produced single-sex, male cercariae without egg production and associated pathology. Cercariae were their intermediate snail hosts accordance with principles good manufacturing practice (GMP). The application GMP an unconventional process is...
Summary Patients with haematological malignancies are at risk for invasive fungal diseases ( IFD ). A survey was conducted in all Dutch academic haematology centres on their current diagnostic, prophylactic and therapeutic approach towards the context of azole‐resistance. In 8 centres, a haematologist microbiologist filled questionnaire that focused different subgroups patients. Fungal prophylaxis during neutropaenia directed against Candida consisted fluconazole and/or amphotericin B...