Two microsporidia species have been shown to infect Apis mellifera, Nosema apis and ceranae. This work presents evidence that N. ceranae infection significantly suppresses the honey bee immune response, although this effect was not observed following with apis. Immune suppression would also increase susceptibility other pathogens senescence. Despite importance of both in health, there is no information about their on bees' system present results can explain different virulence between...

Honeybees Apis mellifera are important pollinators of wild plants and commercial crops. For more than a decade, high percentages honeybee colony losses have been reported worldwide. Nutritional stress due to habitat depletion, infection by different pests pathogens pesticide exposure has proposed as the major causes. In this study we analyzed how nutritional affects strength health. Two groups colonies were set in Eucalyptus grandis plantation at beginning flowering period (autumn),...

Due to their social behaviour, honey bees can be infected by a wide range of pathogens including the microsporidia Nosema ceranae and bacteria Paenibacillus larvae . The use probiotics as food additives for control or prevention infectious diseases is widely used approach improve human animal health. In this work, we generated mixture four Lactobacillus kunkeei strains isolated from gut microbial community bees, evaluated its potential beneficial effect on adult bees. Its administration in...

Uropathogenic Escherichia coli (UPEC) is the most common agent of urinary tract infection (UTI). The classic model pathogenesis proposes ascent UPEC by urethra and external adherence to urothelium. Recently, ability invade urothelial cells form intracellular bacterial communities (IBCs) has been described.The objective present study was determine presence bacteria (IB) in children with UTI caused E. characterize its virulence attributes relation clinical outcomes. One hundred thirty-three...

The formation of intracellular bacterial communities (IBC) has been proposed as a new pathogenic model for urinary tract infections. Scarce reports describe this phenomenon in humans. We the presence IBC uroepithelial cells child with recurrent Urine specimen was collected from Escherichia coli UTI and analyzed by light confocal laser scanning microscopy (CLSM). capability strain to produce infection bladder tissue confirmed mice models. phylogenetic group, virulence factors genes, its...

Glyphosate is the most used pesticide around world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts behavior, cognitive, sensory and developmental abilities, use continues to grow. Recent shown that it also alters composition of bee gut microbiota. In this study we explored impact chronic exposure sublethal doses glyphosate microbiota effects immune response, infection by Nosema ceranae Deformed wing virus (DWV) survival. combined...

The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention several diseases that affect animals, such as neonatal calf diarrhoea. selection strains within a collection based on different in vitro vivo assays, which predict their potential. aim this study was to characterise group Lactobacillus spp. isolated from faeces healthy calves using approach assess ability colonise gastrointestinal tract (GIT) calves. Native were evaluated survive...

Proteus mirabilis is one of the most common etiological agents complicated urinary tract infections, especially those associated with catheterization. This related to ability P. form biofilms on different surfaces. pathogen encodes 17 putative fimbrial operons, highest number found in any sequenced bacterial species so far. The present study analyzed role four fimbriae (MR/P, UCA, ATF and PMF) biofilm formation using isogenic mutants. Experimental approaches included migration over catheter,...

Proteus mirabilis(P. mirabilis) is a common etiological agent of urinary tract infections, particularly those associated with catheterization. P. mirabilis efficiently forms biofilms on different surfaces and shows multicellular behavior called 'swarming', mediated by flagella. To date, the role flagella in biofilm formation has been under debate. In this study, we assessed using an isogenic allelic replacement mutant unable to express flagellin. Different approaches were used, such as...

Proteus mirabilis, a common cause of urinary tract infection, produces number different fimbriae, including ambient temperature fimbriae (ATF). These are optimally expressed at 23°C and their contribution to infection has so far remained unknown. In the present study, clinical isolate P. mirabilis an isogenic allelic replacement mutant unable express ATF were tested for ability in ascending model mice. The atf colonised as well wild-type strain was also able outcompete co-challenge...

The aim of this study was to isolate and identify Lactobacillus spp. isolates from faeces a healthy dog, characterize their potential as probiotics in order evaluate possible use for dogs.An vitro approach used the including evaluation pH bile salts tolerance, production antimicrobial substances, biofilm formation on glass polystyrene surfaces, aggregation ability adhesion canine intestinal mucus. survived different conditions, inhibited growth Escherichia coli Clostridium perfringens,...

Urinary tract infections (UTIs) are among the most common bacterial in humans. Proteus mirabilis is an opportunistic pathogen, capable of causing severe UTIs, with serious kidney damage that may even lead to death. Several virulence factors involved pathogenicity this bacterium. Among these, adherence uroepithelium mediated by fimbriae appears be a significant attribute related urovirulence. expresses several types could pathogenesis UTI, including uroepithelial cell adhesin (UCA). In...

Escherichia coli is one of the main etiological agents neonatal calf diarrhea (NCD). The objective this study was to assess presence virulence genes, genetic diversity, and antibiotic resistance mechanisms in E. associated with NCD Uruguay. PCR used intimin, Shiga-like toxin, stable labile enterotoxin genes. Resistance fluoroquinolones oxyimino-cephalosporins estimated on Müller-Hinton agar plates. Further disc-diffusion tests were performed bacterial multi-resistance. PMQR, ESBL, MCR-1,...

Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides control Varroa mites. These compounds can accumulate at high concentrations in apicultural matrices. In work, effects parasitosis/acaricide on genes involved immunity survival were evaluated. Nurse bees infected with N. and/or chronically treated...

Neonatal calf diarrhoea is one of the challenges faced by intensive farming, and probiotics are considered a promising approach to improve calves’ health. The objective this study was evaluate effect potential probiotic lactobacilli on new-born dairy growth, incidence, faecal score, cytokine expression in blood cells, immunoglobulin A (IgA) levels plasma faeces, pathogen abundance faeces. Two vivo assays were conducted at same farm two annual calving seasons. Treated calves received daily...