A5031223616- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Reproductive Physiology in Livestock
- Reproductive biology and impacts on aquatic species
- Tissue Engineering and Regenerative Medicine
- Tree Root and Stability Studies
- Ubiquitin and proteasome pathways
- Soil Mechanics and Vehicle Dynamics
- Plant Toxicity and Pharmacological Properties
- Urinary Bladder and Prostate Research
- Animal Genetics and Reproduction
- Polymer-Based Agricultural Enhancements
- Selenium in Biological Systems
- Genetic and phenotypic traits in livestock
- Xenotransplantation and immune response
Universidade Federal de São Paulo
2019-2025
State University of Norte Fluminense
2012-2020
Varicocele is associated with a progressive decrease in male fertile potential, but it has yet to be determined if the duration of varicocele altered sperm functional quality. This experimental study investigated time-dependent effects on spermatogenesis, parameters, and traits. Thirty-five mature Wistar rats (200 ± 25 g) were included. After 2 weeks adaptation, randomly divided (n = 7/group) into control, months sham (sham-2), (VCL-2), 4 (sham-4), (VCL-4) groups. Cauda epididymides incised...
We aimed to evaluate the effects of L-arginine (L-arg) in quality vitro heparin-induced capacitation cryopreserved bovine spermatozoa and its on IVP.The experimental groups were: Control 0 hour without pre-capacitation, sperm capacitated for 30 min absence COC with heparin (Control min), 1 mM L-arg + heparin.The pattern was evaluated by chlortetracycline assay integrity plasma membrane (PM) acrosome (AM) association Hoescht 33342 propidium iodide.Further, we assess rate blastocysts...
We aimed to elucidate whether NO acts in vitro sperm capacitation bovine via cGMP/PKG1 pathway. For this, cryopreserved were capacitated with 20 µg/ml heparin (Control) plus treatments: 1 mM L-arginine (L-arg, precursor), 50 µM Rp-8-Bromo-β-phenyl-1,N2 -ethenoguanosine-3',5'-cyclic monophosphorothioate (Rp-8-Br-cGMPS, selective inhibitor of the binding site for cGMP PKG1), 2-Phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO, scavenger), and combinations L-arg + RP-8-Br-cGMPS PTIO....
Male infertility is one important factor among the multifactorial causes of couple infertility, being oxidative stress main related sources. Sperm a specialized cell extremely susceptible to stress. To understand and mitigate this event, many studies have used different antioxidants, orally or in vitro supplementation, trying improve sperm quality function. Considering extensive available literature regarding approaches attempts solve male fertility issues, aim review evaluating effects...
Summary This study aimed to assess the effects of inhibition nitric oxide synthase (NOS) on events that modulate bovine in vitro oocyte maturation. Cumulus–oocyte complexes (COCs) were cultured with hemisections (HSs) follicular walls a maturation medium supplemented different concentrations (0.1–10.0 mM) N ω -nitro- l -arginine methyl ester hydrochloride ( -NAME). Controls consisted COCs presence (+HSs) or absence HSs (–HSs) no additional -NAME supplementation. The following parameters...
Tema e Alcance: A estimativa da fertilidade do reprodutor é uma ferramenta importante na escolha macho, não só porque reflete o estado individual reprodutor, mas também dito resultado influencia futuro rebanho, a presente revisão aborda assunto no macho bovino desde perspectiva biotecnologia sêmen. Características: descreve as características seminais que influenciam potencial reprodutivo apresenta de forma geral técnicas laboratoriais rotineiras inovadoras avaliação espermatozoides....
Nuclear maturation of the oocytes, characterised by a resumption meiosis, occurs at end growing phase in preovulatory follicles, after peak gonadotropins. The mechanisms and substances involved, which gonadotropins lead to final nuclear maturation, are still unknown. withdrawal immature oocytes from follicular environment is sufficient trigger spontaneous hindering study these events vitro model. However, co-culture with hemi-sections (HS) walls holds meiosis arrest therefore an alternative...
A L-arginina (L-arg) é o principal precursor da síntese do NO, contudo, precursora também de creatina, agmatina, ureia, proteica, L-ornitina, poliaminas, L-prolina e L-glutamato