A5026372875- Liver Disease Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Liver physiology and pathology
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Disease and Transplantation
- Liver Diseases and Immunity
- Radiomics and Machine Learning in Medical Imaging
- Artificial Intelligence in Healthcare
- Brain Tumor Detection and Classification
- Tissue Engineering and Regenerative Medicine
- Endoplasmic Reticulum Stress and Disease
- Drug-Induced Hepatotoxicity and Protection
- Receptor Mechanisms and Signaling
- Organ Transplantation Techniques and Outcomes
- Clinical Nutrition and Gastroenterology
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Hepatitis B Virus Studies
- Lipid metabolism and biosynthesis
- Hepatocellular Carcinoma Treatment and Prognosis
- Heat shock proteins research
- Pharmacogenetics and Drug Metabolism
- Microtubule and mitosis dynamics
- Peptidase Inhibition and Analysis
TU Dortmund University
2011-2025
Leibniz Research Centre for Working Environment and Human Factors
2011-2025
Histological alterations often constitute a fingerprint of toxicity and diseases. The extent to which these are cause or consequence compromised organ function, the underlying mechanisms involved is matter intensive research. In particular, liver disease associated with altered tissue microarchitecture, in turn may compromise perfusion functionality. Research this field requires development orchestration new techniques into standardized processing pipelines that can be used reproducibly...
Acetaminophen (APAP) overdose remains a frequent cause of acute liver failure, which is generally accompanied by increased levels serum bile acids (BAs). However, the pathophysiological role BAs elusive. Herein, we investigated in APAP-induced hepatotoxicity.We performed intravital imaging to investigate BA transport mice, quantified endogenous concentrations mice and patients with APAP overdose, analyzed tissue mass spectrometry MALDI-mass imaging, assessed integrity blood-bile barrier...
Abstract Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by inflammation and progressive fibrosis of the biliary tree. The majority PSC patients suffer from concomitant inflammatory bowel (IBD), which has been suggested to promote development progression. However, molecular mechanisms intestinal may aggravate remain incompletely understood. Here, we employ an IBD-PSC mouse model investigate impact colitis on bile acid metabolism injury. Unexpectedly,...
Cholemic nephropathy (CN) is a severe complication of cholestatic liver diseases for which there no specific treatment. We revisited its pathophysiology with the aim identifying novel therapeutic strategies.
Mouse models are frequently used to study chronic liver diseases (CLDs). To assess their translational relevance, we quantified the similarity of commonly mouse human CLDs based on transcriptome data. Gene‐expression data from 372 patients were compared with acute and consisting 227 mice, additionally nine published gene sets models. Genes consistently altered in humans mice mapped cell types single‐cell RNA‐sequencing validated by immunostaining. Considering top differentially expressed...
Background and Aims Small‐molecule flux in tissue microdomains is essential for organ function, but knowledge of this process scant due to the lack suitable methods. We developed two independent techniques that allow quantification advection (flow) diffusion individual bile canaliculi interlobular ducts intact livers living mice, namely fluorescence loss after photoactivation intravital arbitrary region image correlation spectroscopy. Approach Results The results challenge prevailing...
Mouse models of non-alcoholic fatty liver disease (NAFLD) are required to define therapeutic targets, but detailed time-resolved studies establish a sequence events lacking. Here, we fed male C57Bl/6N mice Western or standard diet over 48 weeks. Multiscale characterization was performed using RNA-seq, histopathology, immunohistochemistry, intravital imaging, and blood chemistry; the results were compared human disease. Acetaminophen toxicity ammonia metabolism additionally analyzed as...
Partial liver removal is an important therapy option for cancer. In most patients within a few weeks, the able to fully regenerate. some patients, however, regeneration fails with often severe consequences. To better understand control mechanisms of regeneration, experiments in mice were performed, guiding creation spatiotemporal 3D model regenerating liver. The represents cells and blood vessels entire lobe, macroscopic subunit. could reproduce experimental data only if biomechanical growth...
Despite progress understanding the mechanisms underlying tumor spread, metastasis remains a clinical challenge. We identified choline-producing glycerophosphodiesterase, EDI3 and reported its association with metastasis-free survival in endometrial cancer. also observed that silencing slowed cell migration other cancer-relevant phenotypes vitro. Recent work demonstrated high expression ER-HER2+ breast cancer compared to molecular subtypes. Silencing cells significantly reduced vitro...
Many physiological processes and pathological phenomena in the liver tissue are spatially heterogeneous. At a local scale, biomarkers can be quantified along axis of blood flow, from portal fields (PFs) to central veins (CVs), i.e., zonated form. This requires detecting PFs CVs. However, manually annotating these structures multiple whole-slide images is tedious task. We describe evaluate fully automated method, based on convolutional neural network, for simultaneously CVs single stained...
Abstract Small-molecule flux in tissue-microdomains is essential for organ function, but knowledge of this process scant due to the lack suitable methods. We developed two independent techniques that allow quantification advection (flow) and diffusion individual bile canaliculi interlobular ducts intact livers living mice, namely Fluorescence Loss After Photoactivation (FLAP) Intravital Arbitrary Region Image Correlation Spectroscopy (IVARICS). The results challenge prevailing...
The process of liver regeneration is unique in regard to other organs. It presents the ability recover up 70% organ mass a very short time frame few days, while also preserving quite efficiently fine and architecture multicellular components (e.g. hepatocytes, sinusoiodal cells, etc) which fundamental for its functions. Our investigations have shown, based on computer models generated using series confocal images from regenerating after CCl4 induced damage that novel aspect hepatocytes...
Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by inflammation and progressive fibrosis of the biliary tree. PSC shows striking association with inflammatory bowel up to 80% patients suffering from concomitant colitis. Although this has been known for decades, molecular mechanisms which intestinal may interact remain largely unknown. Here, we developed an IBD-PSC mouse model simulating impact colitis on bile acid metabolism injury. Unexpectedly,...