A5014796293- Air Quality and Health Impacts
- Nanoparticles: synthesis and applications
- Inhalation and Respiratory Drug Delivery
- Air Quality Monitoring and Forecasting
- Energy and Environment Impacts
- Climate Change and Health Impacts
- Neonatal Respiratory Health Research
- Occupational and environmental lung diseases
- Indoor Air Quality and Microbial Exposure
- Heavy Metal Exposure and Toxicity
- Pesticide Exposure and Toxicity
- Pesticide and Herbicide Environmental Studies
- Nanoparticle-Based Drug Delivery
- Carcinogens and Genotoxicity Assessment
- Toxic Organic Pollutants Impact
- Atmospheric chemistry and aerosols
- Animal testing and alternatives
- Microplastics and Plastic Pollution
- Immune Response and Inflammation
- Noise Effects and Management
- Graphene and Nanomaterials Applications
- Genomics, phytochemicals, and oxidative stress
- Advanced Nanomaterials in Catalysis
- Electrostatics and Colloid Interactions
- Ion Transport and Channel Regulation
Université Paris Cité
2014-2024
Unit of Functional and Adaptive Biology
2014-2024
Centre National de la Recherche Scientifique
2012-2023
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2014-2020
Sorbonne Paris Cité
2011-2018
Délégation Paris 7
2009-2018
Sorbonne Université
2004-2018
Délégation Paris 6
2017
Inserm
2001
Background: There is a strong need for laboratory in vitro test systems the toxicity of airborne particulate matter and nanoparticles. The measurement oxidative stress potential offers promising way forward. Objectives:Aworkshop was convened involving leading workers from field order to review available methods generate Consensus Statement. Discussions: Workshop participants summarised their own research activities as well discussion relative merits different methods. Conclusions: In have an...
Diesel exhaust particles (DEP) induce a proinflammatory response in human bronchial epithelial cells (16HBE) characterized by the release of cytokines after activation transduction pathways involving MAPK and transcription factor NF-kappaB. Because cellular effects induced DEP are prevented antioxidants, they could be mediated reactive oxygen species (ROS). Using fluorescent probes, we detected ROS production nasal exposed to native DEP, organic extracts (OE-DEP), or several polyaromatic...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
The involvement of diesel exhaust particles (DEPs) in respiratory diseases was evaluated by studying their effects on two vitro models human airway epithelial cells. cytotoxicity DEPs, phagocytosis, and the resulting immune response were investigated a bronchial cell line (16HBE14o−) as well nasal cells primary culture. DEP exposure induced time- dose-dependent membrane damage. Transmission electron microscopy showed that DEPs underwent endocytosis translocated through sheet. Flow cytometric...
Abstract Background Increasing environmental and occupational exposures to nanoparticles (NPs) warrant deeper insight into the toxicological mechanisms induced by these materials. The present study was designed characterize cell death carbon black (CB) titanium dioxide (TiO 2 ) NPs in bronchial epithelial cells (16HBE14o- line primary cells) investigate implicated molecular pathways. Results Detailed time course studies revealed that both CB (13 nm) TiO (15 NP exposed exhibit typical...
The uptake of nanoparticles (NPs) by cells remains to be better characterized in order understand the mechanisms potential NP toxicity as well for a reliable risk assessment. Real is still difficult evaluate because adsorption NPs on cellular surface. Here we used two approaches distinguish adsorbed fluorescently labeled from internalized ones. extracellular fluorescence was either quenched Trypan Blue or analyzed using imaging flow cytometry. We this novel technique define inside cell...
It has been shown that nanomaterials (NMs) are able to translocate secondary tissues one of the important being kidneys. Oxidative stress implicated as a possible mechanism for NM toxicity, hence effects on human renal proximal tubule epithelial cells (HK-2) treated with panel engineered consisting two zinc oxide particles (ZnO - coated 110 and uncoated 111), multi walled carbon nanotubes (MWCNT) (NM 400 402), silver 300) five TiO2 NMs 101, NRCWE 001, 002, 003 004) were evaluated. In order...
The lung epithelium constitutes the first barrier against invading pathogens and also a major surface potentially exposed to nanoparticles. In order ensure preserve epithelial function, alveolar compartment possesses local defence mechanisms that are able control bacterial infection. For instance, macrophages professional phagocytic cells engulf bacteria environmental contaminants (including nanoparticles) secrete pro-inflammatory cytokines effectively eliminate bacteria/contaminants....
The contribution of air particles in human cardio-respiratory diseases has been enlightened by several epidemiological studies. However the respective involvement coarse, fine and ultrafine health effects is still unclear. aim present study to determine which size fraction from a chemically characterized background aerosol most important short term biological effect decipher determinants such behaviour.Ambient aerosols were collected at an urban site Paris using four 13-stage low pressure...
We report on the uptake, toxicity and degradation of magnetic nanowires by NIH/3T3 mouse fibroblasts. Magnetic diameters 200 nm lengths comprised between 1 {\mu}m 40 are fabricated controlled assembly iron oxide ({\gamma}-Fe2O3) nanoparticles. Using optical electron microscopy, we show that after 24 h incubation wires internalized cells located either in membrane-bound compartments or dispersed cytosol. fluorescence were identified as late endosomal/lysosomal endosomes labeled with lysosomal...
Epidemiological studies in urban areas have linked increasing respiratory and cardiovascular pathologies with atmospheric particulate matter (PM) from anthropic activities. However, the biological fate of metal-rich PM industrial emissions developed countries remains understudied. Lead toxicity bioaccessibility assessments were therefore performed on a lead recycling plant, using complementary chemical acellular tests toxicological assays, as function size (PM(10-2.5), PM(2.5-1) PM(1))...
Nowadays, effects of fine particulate matter (PM2.5) are well-documented and related to oxidative stress pro-inflammatory response. Nevertheless, epidemiological studies show that PM2.5 exposure is correlated with an increase pulmonary cancers the remodeling airway epithelium involving regulation cell death processes. Here, we investigated components Parisian involved in either induction or inhibition quantified by different parameters apoptosis delineated mechanism underlying this effect.In...
The involvement of particulate matter (PM) in cardiorespiratory diseases is now established developed countries whereas developing areas such as Africa with a high level specific pollution, PM pollution and its effects are poorly studied. Our objective was to characterize the biological reactivity urban African aerosols on human bronchial epithelial cells relation physico-chemical properties identify toxic sources. Size-speciated aerosol chemical composition analyzed Bamako (BK, Mali, 2...
Few experimental techniques allow the analysis of protein corona in situ. As a result, little is known on effects nanoparticles weakly bound proteins that form soft corona. Despite its biological importance, our understanding molecular bases driving formation limited. Here, we show hemoglobin can either hard or silica depending pH conditions. Using cryoTEM and synchrotron-radiation circular dichroism, alter structure stability Molecular dynamics simulation identified structural elements...
We have previously shown that exposure to diesel exhaust particles (DEPs) stimulates human airway epithelial cells secrete the inflammatory cytokines interleukin-8, interleukin-1β, and granulocyte-macrophage colony-stimulating factor (GM-CSF) involved in allergic diseases. In present paper, we studied mechanisms underlying increase GM-CSF release elicited by DEPs using bronchial cell line 16HBE14o−. RT-PCR analysis has an mRNA levels after DEP treatments. Comparison of effects DEPs,...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
The initiation of an inflammatory process is the main adverse effect observed following exposure airway epithelium to nanoparticles (NPs). This study was designed explore pro-inflammatory potential two different NPs similar size but compositions (CB 13 nm and TiO(2) 15 nm) on a human bronchial epithelial cell line (16HBE14o-). expression granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL-6), tumor necrosis alpha (TNFalpha) evaluated in terms mRNA, intracellular...
Aspergillus fumigatus, a saprophytic mould, is responsible for life-threatening, invasive pulmonary diseases in immunocompromised hosts. The role of the airway epithelium involves complex interaction with inhaled pathogen. Antimicrobial peptides direct antifungal and chemotactic activities may boost immune response.The inducible expression defensins by human bronchial epithelial 16HBE cells A549 pneumocyte exposed to A. fumigatus was investigated. Using RT-PCR real time PCR, we showed an...