A5017134647- Gut microbiota and health
- Ion channel regulation and function
- Clostridium difficile and Clostridium perfringens research
- Cardiac electrophysiology and arrhythmias
- Synthesis and Catalytic Reactions
- RNA and protein synthesis mechanisms
- Polymer Nanocomposites and Properties
- Neuroscience and Neural Engineering
- Catalytic C–H Functionalization Methods
- biodegradable polymer synthesis and properties
- Polymer crystallization and properties
- Nicotinic Acetylcholine Receptors Study
- Child Nutrition and Feeding Issues
- Chronic Lymphocytic Leukemia Research
- Protein Degradation and Inhibitors
- Calcium signaling and nucleotide metabolism
- Antibiotic Resistance in Bacteria
- Phosphorus and nutrient management
- Crystal structures of chemical compounds
- Autoimmune Bullous Skin Diseases
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Metal complexes synthesis and properties
- Advanced Electron Microscopy Techniques and Applications
- Epigenetics and DNA Methylation
Sichuan Agricultural University
2024
Ministry of Agriculture and Rural Affairs
2024
Agro-Environmental Protection Institute
2024
Zhongnan Hospital of Wuhan University
2023
Wuhan University
2023
Princeton University
2020-2023
Kunming Institute of Zoology
2017-2021
Chinese Academy of Sciences
2009-2021
University of Chinese Academy of Sciences
2018-2021
Tsinghua University
2015-2019
Significance Membrane proteins (MPs), encoded by ∼30% of the coding genes, play vital roles in numerous physiological processes. MPs are targets more than half FDA-approved drugs. High-resolution structural studies functional membrane under near-physiological conditions required to provide an in-depth mechanistic understanding and facilitate drug discovery. To embed into liposomes represents a strategy mimic native conditions. Here we present convenient workflow for cryo-EM analysis...
Although conventional genetic modification approaches for protein knockdown work very successfully due to the increasing use of CRISPR/Cas9, effective techniques achieving depletion in adult animals, especially large animals such as non-human primates, are lacking. Here, we report a chemical approach based on PROTACs technology that efficiently and quickly knocks down FKBP12 (12-kDa FK506-binding) globally vivo. Both intraperitoneal oral administration led rapid, robust, reversible...
Voltage-gated calcium (Cav) channels mediate Ca2+ influx in response to membrane depolarization, playing critical roles diverse physiological processes. Dysfunction or aberrant regulation of Cav can lead life-threatening consequences. Cav-targeting drugs have been clinically used treat cardiovascular and neuronal disorders for several decades. This review aims provide an account recent developments the structural dissection channels. High-resolution structures significantly advanced our...
A novel approach to access ortho iodinated phenols using cyclic hypervalent iodine reagents through palladium(II) catalyzed C-H activation has been developed weak coordination. The reaction showed excellent regioselectivity, reactivity and good functional group tolerance. unique mechanism was proposed.
Inflammatory bowel disease (IBD) is an immunologically mediated and may be caused by abnormal immunological response to gut microbes. Although several studies on the ecological changes associated with IBD, such as community diversities, were reported, no previous have investigated in spatial heterogeneity mechanism of assembly microbiota IBD. In present study, we first applied Taylor's power law extensions compare between microbial communities IBD patients those healthy individuals. We found...
Abstract The R-type voltage-gated Ca 2+ (Ca v ) channels 2.3, widely expressed in neuronal and neuroendocrine cells, represent potential drug targets for pain, seizures, epilepsy, Parkinson’s disease. Despite their physiological importance, there have lacked selective small-molecule inhibitors targeting these channels. High-resolution structures may aid rational design. Here, we report the cryo-EM structure of human 2.3 complex with α2δ−1 β3 subunits at an overall resolution 3.1 Å. is nearly...
Abstract An efficient and convenient cobalt‐promoted annulation reaction has been established for the synthesis of biologically relevant phosphaisoquinolin‐1‐ones, which represents first example a transition‐metal‐promoted C−H/N−H functionalization aryl phophinamides with allenes. Through this strategy, three different types novel phosphaisoquinolin‐1‐one derivatives can be readily prepared in good yields. This new strategy exhibited several advantages, including easily accessible starting...
A structure-hopping strategy was applied to discover a series of novel 4-aminoquinoline-3-carboxamide derivatives as potent, reversible BTK inhibitors. Compared the previously described cinnoline scaffold compounds, 4-aminoquinoline analogues showed significantly improved drug-like properties, especially in their aqueous solubility. The most potent compound, 25, displayed stronger inhibitory effect on both BTKWT (IC50 = 5.3 nM) and BTKC481S 39 nM). In rodent collagen-induced arthritis model,...
The emergence and spread of antibiotic resistance have become emerging threats to human health. gut is a large reservoir for genes. resistome may be influenced by many factors, but the consumption antibiotics at both individual country level should one most significant factors. Previous studies suggested that different populations vary, lack quantitative characterization supported with relatively datasets. In this study, we filled gap analyzing dataset 1,267 samples America, China, Denmark,...
Background: The implications of gut microbiome to obesity have been extensively investigated in recent years although the exact mechanism is still unclear. question whether or not influences assembly has addressed. significant because it fundamental for investigating diversity maintenance and stability microbiome, latter should hold a key understanding etiological obesity. Methods: In this study, we adopt dual neutral theory modeling strategy address from both species community perspectives,...
This paper describes the hydrothermal synthesis, full characterization, and architectural diversity of three intriguingly bioactive cobalt–organic frameworks, namely, 3D [Co(HL − )2(BPY)] n ·4nH2O (1), 2D )2(BPE)] (2), )2(DPP)] (3) coordination polymers, synthesized through a mixed ligand strategy using H 2 L (1-H-indazole-3-carboxylic acid) as main structural block flexible bipyridine its derivatives (BPY = 4,4′-bipydine, BPE 1,2-bis(4-pyridyl)ethane, DPP 1,3-bis(4-pyridyl)propane)...
Abstract Thickened water and thickened milk are frequently used in dysphagia management. However, there is still lack of information regarding the correlation between their measured viscosity sensory viscosity. In this work, relationship was investigated by rheological measurements a series tactile tests. Both magnitude estimation (ME) triangle protocols were for evaluation. The data indicated that both shear thinning, but obviously higher than same amount thickeners. For participants...
Colorectal cancer (CRC) is the third commonest malignant tumor. Previous studies have revealed that composition change of human gut microbiome, measured by community diversity, associated with progression CRC. However, a further question, whether or not mechanism assembly and diversity maintenance microbiome influenced CRC has been addressed. To address this we applied Hubbell's neutral theory for biodiversity to reanalyze dataset from Wang et al.'s (2012) study sampled 46 patients 56...
The melting and recrystallization behavior of poly(ethylene terephthalate) (PET)/SiO2 nanocomposites after isothermal crystallization from the melt was studied by step-scan differential scanning calorimetry (SDSC). influences SiO2 contents, temperature, time on process were examined. Two endotherms (in SDSC CP.A curves, reversible part) one exotherm CP.IsoK irreversible PET/SiO2 observed during process. All are ascribed to melting–recrystallization mechanism. low-temperature endotherm is...
Cystic fibrosis (CF) is a hereditary disease that characterized by defective mucociliary clearance, airway obstruction, chronic infection, and persistent inflammation. pulmonary exacerbation (CFPE) majorly causes the morbidity of CF patients. Although has been demonstrated to change composition lung microbial community, previous studies have not made efforts study differences in mechanism assembly diversity maintenance community In this study, we applied neutral theory biodiversity...
That both stochastic neutral and deterministic niche forces are in effect shaping the community assembly diversity maintenance is becoming an increasingly important consensus. However, assessing effects of disease on balance between two human microbiome has not been explored to best our knowledge. In this article, we applied a hybrid model address issue by analyzing potential HIV infection gut adopted further step multimodality testing improve interpretation their model. Our study revealed...
The melting and recrystallization behavior of Poly(ethylene terephthalate) (PET)/SiO2 nanocomposites after isothermal crystallization from the melt was studied by Step-scan differential scanning calorimetry (SDSC). influence SiO2 contents, temperature time on process were examined. Two endotherms(in SDSC CP.A curves, reversible part) one exotherm (in CP.IsoK irreversible part)of PET/SiO2 observed during process. This ascribes to melting-recrystallization mechanism .The low endotherm...
of the mechanism per se has been very limited.Traditionally, there have two diametrically opposite opinions on this issue, i.e., deterministic niche theory and stochastic neutral [21-26] extensive investigations conducted, including reconciling both theories in various frameworks hybrid models macro ecology plants animals.The forces that govern formation micro biota is supposed to be controlled by a number factors, such as host species, genotype, diet, health interactions between microbes...