A5053053735- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Weed Control and Herbicide Applications
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Seed Germination and Physiology
- Flowering Plant Growth and Cultivation
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Lipid metabolism and biosynthesis
- Nitrogen and Sulfur Effects on Brassica
- Plant-Microbe Interactions and Immunity
- Plant and animal studies
- Plant Gene Expression Analysis
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Agronomic Practices and Intercropping Systems
- Genomics, phytochemicals, and oxidative stress
- Molecular Biology Techniques and Applications
- Allelopathy and phytotoxic interactions
- Plant Diversity and Evolution
- Plant Pathogens and Fungal Diseases
- Postharvest Quality and Shelf Life Management
Edward T. Schafer Agricultural Research Center
2015-2024
United States Department of Agriculture
2002-2023
Wells Fargo (United States)
2022-2023
Agricultural Research Service
2011-2022
Bioscience Research
2005-2015
North Dakota State University
2012
Bioscience (China)
2009
Washington State University
1997-2002
University of California, Riverside
1995-2000
Necrotrophic pathogens live and feed on dying tissue, but their interactions with plants are not well understood compared to biotrophic pathogens. The wheat Snn1 gene confers susceptibility strains of the necrotrophic pathogen Parastagonospora nodorum that produce SnTox1 protein. We report positional cloning Snn1, a member wall-associated kinase class receptors, which known drive pathways for resistance. Recognition by activates programmed cell death, allows this necrotroph gain nutrients...
Dormancy of buds is a critical developmental process that allows perennial plants to survive extreme seasonal variations in climate. transitions underground crown the model herbaceous weed leafy spurge were investigated using 23 K element cDNA microarray. These data represent first large-scale transcriptome analysis dormancy an species. Crown collected monthly from August through December, over five year period, used monitor changes during transitions. Nearly 1,000 genes...
LapA RNAs, proteins, and activities increased in response to systemin, methyl jasmonate, abscisic acid (ABA), ethylene, water deficit, salinity tomato (Lycopersicon esculentum). Salicylic inhibited wound-induced increases of RNAs. Experiments using the ABA-deficient flacca mutant indicated that ABA was essential for wound systemin induction LapA, acted synergistically induce gene expression. In contrast, pin2 (proteinase inhibitor 2) not dependent on exogenous ABA. Whereas both le4 (L....
Reliable reference selection for the accurate quantification of gene expression under various experimental conditions is a crucial step in qRT-PCR normalization. To date, only few housekeeping genes have been identified and used as tea plant. The validity those are not clear since their stabilities rigorously examined. identify more appropriate studies on plant, we examined stability 11 candidate from three different sources: orthologs Arabidopsis traditional stably expressed whole-genome...
ABSTRACT Crown buds of field‐grown leafy spurge ( Euphorbia esula L.) were examined to determine relationships between carbohydrate metabolism and gene expression throughout para‐, endo‐, eco‐dormancy during the transition from summer, autumn, winter, respectively. The data indicates that endo‐dormancy plays a role in preventing new shoot growth autumn winter. Cold temperature was involved breaking endo‐dormancy, inducing flowering competence, inhibiting growth. An inverse relationship...
Abstract Modern high-throughput molecular and analytical tools offer exciting opportunities to gain a mechanistic understanding of unique traits weeds. During the past decade, tremendous progress has been made within weed science discipline using genomic techniques deeper insights into weedy such as invasiveness, hybridization, herbicide resistance. Though adoption newer “omics” proteomics, metabolomics, physionomics slow, applications these omics platforms study plants, especially...
Maize seedlings contain high amounts of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), and the effect DIMBOA is directly associated with multiple insect-resistance against insect pests such as Asian corn borer leaf aphids. Although numerous genetic loci for insect-resistant traits have been identified, little known about controls regarding content. In this study, best linear unbiased prediction (BLUP) values content in two ecological environments across 310 maize inbred lines were...
Dormancy and subsequent regrowth of adventitious buds is a critical physiological process for many perennial plants. We have used the expression hormone cell cycle-responsive genes as markers to follow this in leafy spurge (Euphorbia esula). In conjunction with earlier studies, we show that loss mature leaves results decreased sugar levels increased gibberellin perception underground buds. Gibberellin sufficient induction S phase-specific but not M gene expression. Loss both apical axillary...
Quantitative real-time polymerase chain reaction (qRT-PCR) is the most important tool in measuring levels of gene expression due to its accuracy, specificity, and sensitivity. However, accuracy qRT-PCR analysis strongly depends on transcript normalization using stably expressed reference genes. The aim this study was find internal genes for various experimental conditions seed, adventitious underground bud, other organs leafy spurge. Eleven candidate (BAM4, PU1, TRP-like, FRO1, ORE9, BAM1,...
Leafy spurge (Euphorbia esula L.) is a noxious perennial weed that produces underground adventitious buds, which are crucial for generating new vegetative shoots following periods of freezing temperatures or exposure to various control measures. It also capable flowering and producing seeds, but requires vernalization in some cases. DORMANCY ASSOCIATED MADS-BOX (DAM) genes have been proposed play direct role the transition winter-induced dormancy maintenance through regulation FLOWERING...
Plant model systems have contributed greatly to the dramatic progress in understanding fundamental aspects of plant biology. Using weeds will also help facilitate focused funding and research weed science community. Criteria for developing require attention weedy characteristics that impart economic losses a wide geographic distribution, attributes present potential political scientific support. Expressed sequence tag (EST) databases are most practical approach identifying new genes...
Signals from both leaves and apical or axillary meristems of leafy spurge are known to inhibit root bud growth. To test the hypothesis that carbohydrates growth regulators affect growth, decapitated plants were hydroponically treated with glucose, sucrose, gibberellic acid (GA), abscisic (ABA), 1-naphthaleneacetic (NAA), 6-benzylaminopurine (BA), a GA biosynthesis inhibitor, paclobutrazol. Both glucose sucrose caused suppression at concentrations 30 mM. The inhibitory effect was counteracted...
Genomics programs in the weed science community have not developed as rapidly that of other crop, horticultural, forestry, and model plant systems. Development genomic resources for selected weeds are expected to enhance our understanding biology, just they In this report, we describe development, characteristics, information gained from an expressed sequence tag (EST) database perennial leafy spurge. ESTs were obtained using a normalized cDNA library prepared comprehensive collection...
Leafy spurge (Euphorbia esula L.) is an herbaceous weed that maintains a perennial growth pattern through seasonal production of abundant underground adventitious buds (UABs) on the crown and lateral roots. During normal growing season, differentiation bud to shoot inhibited by physiological factors external affected structure; phenomenon referred as paradormancy. Initiation from paradormant UABs can be accomplished removal aerial shoots (hereafter paradormancy release).In this study,...
Abstract The nature of the vegetative to reproductive transition in shoot apical meristem Camelina sativa summer annual cultivar CO46 and winter Joelle was confirmed by treating seedlings with or without 8 weeks vernalization. True their life cycle classification, required a vernalization treatment induce bolting flowering, whereas did not. In this study, whole genome sequence, RNAseq, resequencing PCR‐amplified transcripts for key floral repressor were used better understand factors...
Maize is a cold-sensitive crop, and it exhibits severe retardation of growth development when exposed to cold snaps during right after seedling emergence. Although different agronomic, physiological, molecular approaches have been tried overcome the problems related stress in recent years, mechanisms causing resistance maize are still unclear. Screening breeding varieties for may be sustainable option boost production under low-temperature environments. Herein, seedlings 39 genotypes were...