A5029620295- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Genetics and Plant Breeding
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Bioenergy crop production and management
- Botanical Research and Chemistry
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Agricultural Productivity and Crop Improvement
- Genetic and Environmental Crop Studies
- Agriculture, Plant Science, Crop Management
- Plant Disease Management Techniques
- Phytase and its Applications
- Nematode management and characterization studies
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Transgenic Plants and Applications
Washington State University
2007-2025
North Dakota State University
2012-2024
ORCID
2021
International Center for Agricultural Research in the Dry Areas
2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Dakota State University
2014-2019
United States Department of Agriculture
2016
Agricultural Research Service
2016
Edward T. Schafer Agricultural Research Center
2016
Curtin University
2016
Stem rust caused by Puccinia graminis f. sp. tritici was among the most devastating diseases of barley in northern Great Plains U.S. and Canada before deployment stem rust-resistance gene Rpg1 1942. Since then, has provided durable protection against losses widely grown cultivars (cvs.). Extensive efforts to clone synteny with rice excellent flanking markers but failed yield because it does not seem exist rice. Here we report map-based cloning characterization . A high-resolution genetic map...
Necrotrophic pathogens live and feed on dying tissue, but their interactions with plants are not well understood compared to biotrophic pathogens. The wheat Snn1 gene confers susceptibility strains of the necrotrophic pathogen Parastagonospora nodorum that produce SnTox1 protein. We report positional cloning Snn1, a member wall-associated kinase class receptors, which known drive pathways for resistance. Recognition by activates programmed cell death, allows this necrotroph gain nutrients...
We isolated the barley stem rust resistance genes Rpg5 and rpg4 by map-based cloning. These are colocalized on a 70-kb genomic region that was delimited recombination. The gene consists of an unusual structure encoding three typical plant disease protein domains: nucleotide-binding site, leucine-rich repeat, serine threonine kinase. predicted RPG5 has two putative transmembrane sites possibly involved in membrane binding. is expressed at low but detectable levels. Posttranscriptional...
Plant peroxisomes perform multiple vital metabolic processes including lipid mobilization in oil-storing seeds, photorespiration, and hormone biosynthesis. Peroxisome biogenesis requires the function of peroxin (PEX) proteins, PEX10, a C 3 HC 4 Zn RING finger peroxisomal membrane protein. Loss PEX10 causes embryo lethality at heart stage. We investigated with conditional sublethal mutants. Four T-DNA insertion lines expressing pex10 dysfunctional were created an Arabidopsis WT background...
The rpg4 gene confers recessive resistance to several races of wheat stem rust (Puccinia graminis f. sp. tritici) and Rpg5 provides dominant against isolates the rye (P. secalis) in barley. genes are tightly linked on chromosome 5H, positional cloning using high-resolution populations clearly separated genes, unambiguously identifying Rpg5; however, identity remained unclear. High-resolution genotyping critical recombinants at rpg4/Rpg5 locus, designated here as rpg4-mediated locus (RMRL)...
Invasive microbes causing diseases such as sudden oak death negatively affect ecosystems and economies around the world. The deployment of resistant genotypes for combating introduced typically relies on breeding programs that can take decades to complete. To demonstrate how this process be accelerated, we employed a genome-wide association mapping ca . 1,000 resequenced Populus trichocarpa trees individually challenged with Sphaerulina musiva , an invasive fungal pathogen. Among significant...
Parastagonospora nodorum is a necrotrophic fungal pathogen causing Septoria blotch on wheat. We have identified nine effector–host dominant sensitivity gene interactions, and we cloned three of the effector genes, including SnToxA, SnTox1, SnTox3. Because sexual populations P. are difficult to develop under lab conditions, genome-wide association study (GWAS) best population genomic approach identify regions associated with traits using natural populations. In this article, used global...
Septoria nodorum blotch (SNB), a disease caused by the necrotrophic fungal pathogen Parastagonospora nodorum, is threat to wheat (Triticum aestivum) production worldwide. Multiple inverse gene-for-gene interactions involving recognition of effectors (NEs) sensitivity genes play major roles in causing SNB. One interaction involves gene Snn3 and P. NE SnTox3. Here, we used map-based strategy clone Snn3-D1 from Aegilops tauschii, D-genome progenitor common wheat. contained protein kinase sperm...
The stem-rust-susceptible barley cv. Golden Promise was transformed by Agrobacterium-mediated transformation of immature zygotic embryos with the Rpg1 genomic clone Morex containing a 520-bp 5' promoter region, 4,919-bp gene and 547-bp 3' nontranscribed sequence. Representatives 42 transgenic lines obtained were characterized for their seedling infection response to pathotype Pgt-MCC stem rust fungus Puccinia graminis f. sp. tritici. converted from highly susceptible cultivar into resistant...
Barley net form blotch (NFNB), caused by the necrotrophic fungus Pyrenophora teres f. teres, is a destructive foliar disease in barley-growing regions worldwide. Little known about genetic and molecular basis of this pathosystem. Here, we identified small secreted proteinaceous effector (NE), designated PttNE1, from intercellular wash fluids susceptible barley line Hector after inoculation with P. isolate 0-1. Using recombinant inbred (RIL) population developed cross between...
Soybean cyst nematode (SCN; Heterodera glycines Ichinohe) reproduces on the roots of common bean (Phaseolus vulgaris L.) and can cause reductions in plant growth seed yield. The molecular changes caused by SCN infection are unknown. Identification genetic factors associated with resistance could help development improved varieties high resistance. Gene expression profiling was conducted infected HG type 0 using next generation RNA sequencing technology. Two pinto genotypes, PI533561 GTS-900,...
Spot form net blotch (SFNB), caused by the necrotrophic fungal pathogen Pyrenophora teres f. maculata, is an important foliar disease of barley in major production regions around world. Deployment adequate host resistance challenging because virulence P. maculata highly variable and characterized minor-effect resistances are typically ineffective against diverse populations. A world core collection consisting 2,062 accessions origin genotype were phenotyped at seedling stage with four...
We report genomic regions that significantly control resistance to scald, net form (NFNB) and spot blotch (SFNB) in barley. Barley genotypes from Ethiopia, ICARDA, the United States were evaluated Ethiopia North Dakota State University (NDSU). Genome-wide association studies (GWAS) conducted using 23,549 single nucleotide polymorphism (SNP) markers for disease five environments Ethiopia. For NFNB SFNB, we assessed seedling a glasshouse at NDSU. A large proportion of Ethiopian landraces...
Race TTKSK (Ug99) of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici) is a serious threat to both and barley production worldwide because its wide virulence on many cultivars rapid spread from eastern Africa. Line Q21861 one most resistant barleys known this race. To elucidate genetics resistance in line, we evaluated Q21861/SM89010 (Q/SM) doubled-haploid population for reaction race at seedling stage. Segregation resistance:susceptibility Q/SM lines fit 1:1 ratio (58:71 with...
Summary Genetic and genomics tools to characterize host–pathogen interactions are disproportionately directed the host because of focus on resistance. However, understanding genetics pathogen virulence is equally important has been limited by high cost de novo genotyping species with marker data. Non‐resource‐prohibitive methods that overcome limitation now available through genotype‐by‐sequencing ( GBS ). The use a two‐enzyme restriction‐associated DNA RAD )‐ method adapted for I T orrent...