A5057831967- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Yeasts and Rust Fungi Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Resistance
- Genetics and Plant Breeding
- Plant Pathogens and Fungal Diseases
- Bioenergy crop production and management
- Antifungal resistance and susceptibility
- Reservoir Engineering and Simulation Methods
- Enhanced Oil Recovery Techniques
- Hydraulic Fracturing and Reservoir Analysis
- Soybean genetics and cultivation
- Oil and Gas Production Techniques
- Crop Yield and Soil Fertility
- Plant-Microbe Interactions and Immunity
- Drilling and Well Engineering
- Mycotoxins in Agriculture and Food
- Concrete and Cement Materials Research
- Innovations in Concrete and Construction Materials
- Legume Nitrogen Fixing Symbiosis
- Agriculture, Plant Science, Crop Management
- Particle physics theoretical and experimental studies
- High-Energy Particle Collisions Research
- Advanced Battery Technologies Research
Washington State University
2016-2025
China National Petroleum Corporation (China)
2018-2024
China National Offshore Oil Corporation (China)
2018-2024
Zhejiang University of Technology
2021-2023
Qingdao Agricultural University
2021-2023
Tsinghua University
2019-2022
Xi'an Aeronautical University
2021
Northwestern Polytechnical University
2019
Quality Research
2017-2018
Sichuan Agricultural University
2016-2018
Background The wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, PST) is responsible for significant yield losses in production worldwide. In spite of its economic importance, the PST genomic sequence not currently available. Fortunately Next Generation Sequencing (NGS) has radically improved sequencing speed and efficiency with a great reduction costs compared to traditional technologies. We used Illumina rapidly access highly virulent race 130 (PST-130). Methodology/Principal...
Abstract Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is a global threat to wheat production. Aegilops tauschii , one of the progenitors, carries YrAS2388 locus for resistance on chromosome 4DS. We reveal that encodes typical nucleotide oligomerization domain-like receptor (NLR). The -resistant allele YrAS2388R has duplicated 3’ untranslated regions and characterized alternative splicing in nucleotide-binding domain. Mutation disrupts its synthetic hexaploid...
Abstract Three members of the Puccinia genus, Pucciniatriticina (Pt), P. striiformis f.sp. tritici (Pst), and graminis (Pgt), cause most common often significant foliar diseases wheat. While similar in biology life cycle, each species is uniquely adapted specialized. The genomes Pt Pst were sequenced compared to that Pgt identify distinguishing gene content, determine variation among wheat rust pathogens, other fungi, basidiomycetes, genes significance for infection. had largest genome...
Effective disease management depends on timely and accurate diagnosis to guide control measures. The capacity distinguish between individuals in a pathogen population with specific properties such as fungicide resistance, toxin production virulence profiles is often essential inform approaches. genomics revolution has led technologies that can rapidly produce high-resolution genotypic information define individual variants of species. However, their application complex fungal pathogens...
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important disease of wheat worldwide. Exploring new resistance genes essential for breeding resistant cultivars. PI 182103, a spring landrace originally from Pakistan, has shown high level to stripe rust in fields many years, but the variety have not been studied. To map gene(s) 185 recombinant inbred lines (RILs) were developed cross with Avocet Susceptible (AvS). The RIL population was genotyped simple sequence repeat (SSR)...
Stripe rust of wheat is a serious disease caused by Puccinia striiformis f. sp. tritici (Pst). Growing resistant cultivars the most preferred approach to control disease. To identify genotypes with quantitative trait loci (QTL) for durable resistance stripe rust, 465 winter entries that were presumed have high-temperature adult-plant (HTAP) used in this study. In greenhouse seedling tests seven Pst races, 16 all tested races. The also phenotyped responses at stage under natural infection...
SUMMARY Stripe rust [caused by Puccinia striiformis Westend. f. sp. tritici Eriks. ( Pst )] is a destructive disease of wheat Triticum aestivum L.) worldwide. Genetic resistance the preferred method for control and Yr5 gene, originally identified in spelta var. album , represents major R ) gene that confers all‐stage to all currently known races United States. To identify transcripts associated with ‐mediated incompatible interaction yr5 ‐compatible interaction, Wheat GeneChip was used...
Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage to stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). The HTAP in is durable as the variety been resistant stage since it was introduced from France United States late 1940s. To map quantitative trait loci (QTL) for resistance, an F8 recombinant inbred line (RIL) population cross × Michigan Amber phenotyped response multiple years fields under natural infection with selected Pst races...
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most serious plant diseases worldwide. Resistant cultivars are effective way to control disease. YrTr1 an important rust resistance gene that has been used in wheat breeding programs and represented host differential set identify P. tritici races United States. To map YrTr1, AvSYrTr1NIL was backcrossed its recurrent parent Avocet S (AvS). Seedlings BC 7 F 2 , 3 8 1 populations were tested with YrTr1-avirulent...
Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici, continues to cause severe damage worldwide. Durable resistance is necessary for sustainable control the disease. High-temperature adult-plant (HTAP) resistance, which expresses when weather becomes warm and plants grow older, has been demonstrated be durable. We conducted numerous studies understand molecular mechanisms different types stripe using a transcriptomics approach. Through comparing gene expression patterns with...
Puccinia striiformis causes stripe rust on wheat, barley, and grasses. Natural population studies have indicated that somatic recombination plays a possible role in P. variation. To determine whether can occur, susceptible wheat or barley plants were inoculated with mixed urediniospores of paired isolates striiformis. Progeny selected by passing through series inoculations genotypes. Potential recombinant compared the parental set 18 12 genotypes are used to differentiate races f. sp....
Plant fungal pathogens can rapidly evolve and adapt to new environmental conditions in response sudden changes of host populations agro-ecosystems. However, the genomic basis their adaptation, especially at forma specialis level, remains unclear. We sequenced two isolates each representing Puccinia striiformis f. sp. tritici (Pst) P. hordei (Psh), different formae speciales stripe rust fungus highly adapted wheat barley, respectively. The divergence Pst Psh, estimated start 8.12 million...
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a major threat to wheat production worldwide, especially in the United States. To identify loci for effective stripe rust resistance U.S. wheat, genome-wide association study (GWAS) was conducted using panel of 616 spring cultivars and breeding lines. The accessions this were phenotyped response greenhouse at seedling stage with five predominant highly virulent races Pst different field environments adult-plant 2017...
Stripe (yellow) rust, caused by fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a serious disease of wheat in the United States and many other countries. Growing resistant cultivars has been approved to be best approach for control stripe rust. To determine rust resistance genes U.S. winter breeding lines, we analyzed panel 857 lines genome-wide association study (GWAS) using genotyping multiplexed sequencing (GMS) with molecular markers 18 important or quantitative trait loci...
Puccinia striiformis f. sp. tritici (Pst) causes wheat stripe rust (also called yellow rust, Yr), one of the most important diseases worldwide. Characterization virulence in Pst populations is essential for developing cultivars with effective and durable resistance to control disease. A total 138 races, including 120 races that were not previously reported, identified from collections made Canada, China, Ecuador, Egypt, Italy Mexico 2013–2020 using a set 18 Yr single-gene differentials....
Abstract Background Puccinia striiformis is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little know about its genome genes involved in biology pathogenicity pathogen. We initiated functional genomic research by constructing full-length cDNA determined functions first group sequence comparison clones to reported other fungi. Results A library, consisting 42,240 with an average insert 1.9 kb, was constructed...
Abstract Background The stripe rust pathogen, Puccinia striiformis f. sp. tritici ( Pst ), threats world wheat production. Resistance to is often overcome by pathogen virulence changes, but the mechanisms of variation are not clearly understood. To determine role mutation in previous studies 30 mutant isolates were developed from a least virulent isolate using ethyl methanesulfonate (EMS) mutagenesis and phenotyped for changes. progenitor was sequenced, assembled annotated establishing...
Abstract Key message The GWAS and testing with Yr gene linked markers identified 109 loci including 40 novel for all-stage adult plant stage resistance to stripe rust in 459 US contemporary hard winter wheat genotypes. Stripe is a destructive disease, caused by Puccinia striiformis f. sp. tritici ( Pst ). To identify sources of wheat, panel Great Plains genotypes was evaluated at the seedling against five races field environments Oklahoma, Kansas, Washington. results showed that 7–14% were...
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat worldwide. William Som (WS), an Argentinian spring landrace, has consistently exhibited high level resistance to stripe rust for over 20 years in our field evaluations Washington state, USA. A previous study showed high-temperature adult-plant (HTAP) WS. To map the HTAP quantitative trait loci (QTL) WS, 114 F5-8 recombinant inbred lines (RILs) from cross AvS/WS were evaluated response seven...
Abstract Plant pathogens rapidly evolve in response to changes their hosts and environments, resulting destructive epidemics. The fungus Puccinia striiformis f. sp. tritici ( Pst ), responsible for wheat stripe rust disease, exemplifies such evolutionary dynamics. Host population impose selective pressures on pathogens; however, the selection footprints demographic patterns of are not well understood. In this study, we performed whole‐genome sequencing 69 isolates collected from diverse...