A5083318543- Axon Guidance and Neuronal Signaling
- Erythropoietin and Anemia Treatment
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Pharmacological Effects and Toxicity Studies
- Tissue Engineering and Regenerative Medicine
- Angiogenesis and VEGF in Cancer
- Mast cells and histamine
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Fetal and Pediatric Neurological Disorders
- Spinal Cord Injury Research
- Signaling Pathways in Disease
- Blood properties and coagulation
- Nitric Oxide and Endothelin Effects
- S100 Proteins and Annexins
- Neurological diseases and metabolism
- interferon and immune responses
- Ion Transport and Channel Regulation
- Healthcare and Venom Research
- Cholinesterase and Neurodegenerative Diseases
- Cellular transport and secretion
- Epilepsy research and treatment
University of Seoul
2025
Oxford University Clinical Research Unit
2024
Ulsan College
2005-2019
University of Ulsan
2005-2019
Asan Medical Center
2016
Institute of Cell Biology and Neurobiology
2003
Tohoku University
1998
Abstract Genetically deregulated tumor cells generate vascular channels by vasculogenic mimicry (VM) that is independent of endothelial blood vessels. The morphological characteristics VM and the role EphA2 in formation were evaluated 144 clinical samples gastric adenocarcinoma AGS cancer cell line. It has long been believed consists PAS-positive basement membrane CD31/CD34-negative cells. Interestingly, we found luminal surface form showed reaction, involvement CD31/CD34-positive channels....
Although erythropoietin‑producing human hepatocellular receptor A2 (EphA2) signaling serves an important role in the tumor microenvironment, its contribution to vasculogenic mimicry (VM) formation gastric cancer cells remains unclear. The aim of present study was investigate EphA2 VM induced by cancer‑associated fibroblasts (CAFs). conditioned medium CAFs (CAF‑CM) prepared from 12 patients with adenocarcinoma. evaluated number tubules and intersections following CAF‑CM treatment....
Various factors including some motor proteins regulate microtubule (MT) transport and influence the formation of neuronal processes. Eg5, a slow non-processive (+)-end directed molecule, is expressed in developing differentiated neurons. However, how Eg5 works neurons still elusive. Thus, we treated primary rat cortical neuron cultures with monastrol, specific inhibitor to investigate its role Immature monastrol extended longer processes than control within few hours. After 3 days, immature...
Abstract Although the neuroprotective effects of erythropoietin (EPO) preconditioning are well known, potential postapplied EPO to protect neurons against excitotoxic injury has not been clearly established. Here we show that kainate (KA)‐induced excitotoxicity, which plays a key role in secondary spinal cord injury, decreased neuron survival, inhibited neurite extension, and significantly reduced expression receptors (EpoR) cultured neurons. Posttreatment with for 48 hr protected KA‐induced...
<i>Objective:</i> To investigate the relationship between enteroviral infection and myocardial tissue apoptosis during development of viral myocarditis in a murine model. <i>Methods:</i> C3H/HeJ mice were inoculated with two strains coxsackievirus B3, specifically CVB3 (cardiovirulent Nancy strain) CVB3/0 (noncardiovirulent strain). Mice sacrificed at 4, 7 10 days postinfection (p.i.). Hearts removed, plaque assays RT-PCR performed to detect presence viruses....
Objective To develop an in vitro model analogous to the environment of traumatic spinal cord injury (SCI), authors evaluated change astrogliosis following treatments with kainate and/or scratch, and degree neurite outgrowth after treatment a inhibitor. Methods Astrocytes were obtained from rat cord. Then, 99% cells confirmed be GFAP-positive astrocytes. For chemical injury, treated at different concentrations (10, 50 or 100 µM). mechanical two kinds uniform scratches made using plastic...
Coxsackievirus B (CVB) causes a wide spectrum of human diseases which are closely associated with direct destruction infected cells. We investigated the morphological and biochemical characteristics CPEs in permissive Vero cells caused by different CVB serotypes. Regardless serotype, experienced similar degrees within 24 h postinfection (p.i.). Using both Hoechst 33342 staining transmission electron microscopy, we consistently observed properties apoptosis, heavily condensed nuclei...
The objective of this study was to investigate the underlying molecular mechanisms and therapeutic time window for preventing astrogliosis with erythropoietin (EPO) treatment after in vitro modeled spinal cord injury (SCI).
Journal Article Involvement of Stromal Membrane-Associated Protein (SMAP-1) in Erythropoietic Microenvironment Get access Yo Sato, Sato Department Cell Biology, Institute Development, Aging and Cancer, Tohoku UniversitySeiryomachi, Aobaku, Sendai 980-8575 Search for other works by this author on: Oxford Academic PubMed Google Scholar Hea Nam Hong, Hong Nobuaki Yanai, Yanai Masuo Obinata 2 To whom correspondence should be addressed. Tel: +81-22-717-8483, Fax: +81-22-717-8488, E-mail:...
Hyperphosphorylation of tau is a characteristic feature the neurodegenerative pathology in Alzheimer's disease (AD). Okadaic acid used as research model AD to increase phosphorylation and neuronal death. Using Western blotting, we found that amounts activated PKB[pS-473] inactivated GSK-3β[pS-9] were increased proportion progress okadaic induced phosphorylation. Immunocytochemistry showed immunoreactivity dystrophic neurites cell bodies degenerating neurons after treatment. Double staining...
RNA interference is an important tool for gene silencing. However, its application to primary cultured cells has been limited by low transfection efficiencies. In this work we developed a vector which encodes both siRNA and red fluorescent protein. Using could markedly suppress green protein (GFP) bim endogenous gene. Primary cortical neurons transfected with against doublecortin showed that expression was significantly inhibited in nearly all the neurons. This identifies should be useful...
Abstract Neuronal stimulation was induced in rats by systemic administration of kainic acid (KA) to determine if such is responsible for changes the expression patterns c‐Kit and stem cell factor (SCF) cerebellar synapses between inhibitory interneurons Purkinje cells. Using immunocytochemistry immunoblotting analyses, we demonstrate that receptor tyrosine kinase its ligand SCF are present on pre‐ postsynaptic sides These proteins upregulated during first 48 hr after KA treatment, whereas...
Tetrahydrobiopterin (BH4) is spontaneously released and extracellularly exerts a toxic effect preferentially on catecholamine cells. Its synthesis rate mainly determined by the activity of enzyme GTP cyclohydrolase I (GTPCH). In present study, role melatonin BH4 was using catecholaminergic CATH.a The neurohormone dose-dependently reduced both intracellular extracellular levels. This due to direct inhibition catalytic existing GTPCH down-regulation gene expression. Thus, an effective...
Abstract The changes in the corpus callosum (CC) with age and gender remain largely subject to dispute, which might come from different strategies for analyzing size shape of CC. We have investigated this issue by measuring some variables reflecting spatial orientation CC on magnetic resonance imaging Koreans, minimize individual variances brain. subjects were composed young adults their twenties (51 male, 59 female) elderly sixties seventies (60 71 female). total area CC, length height...