A5012414509- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Supramolecular Self-Assembly in Materials
- Genomics and Chromatin Dynamics
- Lipid Membrane Structure and Behavior
- Polydiacetylene-based materials and applications
- Chemical Synthesis and Analysis
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Surfactants and Colloidal Systems
- Force Microscopy Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Porphyrin and Phthalocyanine Chemistry
- Gallbladder and Bile Duct Disorders
- Nanopore and Nanochannel Transport Studies
- Analytical Chemistry and Chromatography
- Molecular spectroscopy and chirality
- Pediatric Hepatobiliary Diseases and Treatments
- Diatoms and Algae Research
- MRI in cancer diagnosis
- Radiomics and Machine Learning in Medical Imaging
- Enzyme Structure and Function
- Cholangiocarcinoma and Gallbladder Cancer Studies
Sapienza University of Rome
2013-2025
Istituto Pasteur
1998-2014
National Interuniversity Consortium for the Physical Sciences of Matter
2007
Istituto di Metodologie Chimiche
2004
Istituto Superiore di Sanità
2004
University of Bologna
2001-2003
Istituto Nazionale per la Fisica della Materia
2002
Industriale Chimica (Italy)
1993
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTValidity of the nearest-neighbor approximation in evaluation electrophoretic manifestations DNA curvaturePasquale De Santis, Antonio Palleschi, Maria Savino, and Anita ScipioniCite this: Biochemistry 1990, 29, 39, 9269–9273Publication Date (Print):October 2, 1990Publication History Published online1 May 2002Published inissue 2 October 1990https://pubs.acs.org/doi/10.1021/bi00491a023https://doi.org/10.1021/bi00491a023research-articleACS...
Abstract The problem of the abiotic origin RNA from prebiotically plausible compounds remains unsolved. As a potential partial solution, we report spontaneous polymerization 3′,5′‐cyclic GMP in water, formamide, dimethylformamide, and (in water) presence Brønsted base such as 1,8‐diazabicycloundec‐7‐ene. reaction is untemplated, does not require enzymatic activities, thermodynamically favoured selectively yields 3′,5′‐bonded ribopolymers containing many 25 nucleotides. We propose pathway on...
The energy of DNA deformation plays a crucial and active role in its packaging function the cell. Considerable effort has gone into developing methodologies capable evaluating local sequence-directed curvature flexibility chain. These studies thus far have focused on constructs expressly tailored either with anomalous or tracts. Here we demonstrate that these two structural properties can be mapped also along chain “natural” any sequence basis scanning force microscope (SFM) images. To know...
Synthetic vesicles were prepared by mixing anionic and cationic surfactants, aqueous sodium dodecylsulfate with didodecyltrimethylammonium or cetyltrimethylammonium bromide. The overall surfactant content the (anionic/cationic) mole ratios allow one to obtain negatively charged vesicles. In phase diagram, vesicular region is located between a solution phase, lamellar liquid crystalline dispersion, precipitate area. Characterization of was performed electrophoretic mobility, NMR, TEM, DLS we...
Cubosomes are non-lamellar lipid nanoparticles that have drawn a significant attention in the field of nanomedicine due to their tunable properties. However, formation vesicles during preparation cubosomes, and presence mixed bicontinuous cubic phases, may lead artifacts lack correlation between physico-chemical biological characterization. In this work, we formulated cubosomes composed by monoolein as building block triblock copolymer Pluronic® F108 stabilizer, encapsulating three porphyrin...
Type 1 diabetes and thyroid disease are T cell-dependent autoimmune endocrinopathies. The standard substitutive administration of the deficient hormones does not halt process; therefore, development immunotherapies aiming to preserve residual hormonal cells, is crucial importance. PTPN22 C1858T mutation encoding for R620W lymphoid tyrosine phosphatase variant, plays a potential pathophysiological role in autoimmunity. encoded protein Lyp negative regulator cell antigen receptor signaling;...
Background: In recent years, the multi-drug resistance of bacteria and fungi strains has become a worldwide problem. The incidence fungal bacterial infections is experiencing serious growth microorganisms have developed several new mechanisms antibiotic resistance. Methods: Antimicrobial peptides (AMPs) constitute one well-known barrier defense systems plants. They been isolated from roots, seeds, flowers, stems leaves wide variety species activities towards phytopathogens, as well against...
Vesicular entities were obtained by mixing didodecyldimethylammonium bromide and sodium dodecylsulfate in non-stoichiometric ratios. The vesicles bear a positive surface charge, due to the cationic species being excess, adsorb significant amounts of protein, presumably electrostatic interactions. We modulated net charge bovine serum albumin pH observed its binding onto above vesicles. Binding is controlled albumin: it substantial when has negative charges excess negligible, or non-existent,...
Abstract Nucleosomes, the fundamental repeating subunits of all eukaryotic chromatin, are responsible for packaging DNA into chromosomes inside cell nucleus and controlling gene expression. While it has been well established that nucleosomes exhibit higher affinity select sequences, until recently was unclear whether such preferences exerted a significant, genome-wide effect on nucleosome positioning in vivo. For this reason, an increasing interest is arising wide-ranging series experimental...
Abstract We report that 3′,5′‐cyclic CMP undergoes nonenzymatic di‐ and trimerization at 20 °C under dry conditions upon proton or UV irradiation. The reaction involves stacking of the cyclic monomers subsequent polymerization through serial transphosphorylations between stacked monomers. Proton‐ UV‐induced oligomerization demonstrates pyrimidines—similar to purines—might also have taken part in spontaneous generation RNA plausible prebiotic as well an extraterrestrial context. observed...
In autoimmune diseases as Type 1 diabetes, the actual treatment that provides missing hormones is not able, however, to interrupt underlining immunological mechanism. Importantly, novel immunotherapies are exploited protect and rescue remaining hormone producing cells. Among probable targets of immunotherapy, C1858T mutation in PTPN22 gene, which encodes for lymphoid tyrosine phosphatase (Lyp) variant R620W, reveals an autoimmunity related pathophysiological role. Our scope was establish new...
MR venography has been recommended for the evaluation of deep venous thrombosis. The purpose our study was to determine role venography, in particular at level pelvis where other diagnostic modalities show major limitations.Forty-three patients with clinical suspicion thrombosis were examined by means pelvic venography. In all cases, a 2D-TOF sequence used cranial arterial presaturation. selected i.e. when small intraluminal filling defect present, cine-PC addition order exclude presence...
Two diastereomeric cationic surfactants derived from L-proline, in which the second chiral center is a quaternary nitrogen, have been separated and fully characterized. The recognition properties of aggregates formed by two investigated circular dichroism (1)H NMR through deracemization racemic 2-carboxy-2'-dodecyloxy-6-nitrobiphenyl.