Inhibition of α-glucosidase activity may suppress postprandial hyperglycemia. The inhibition kinetic analysis showed that apigenin reversibly inhibited with an IC50 value (10.5 ± 0.05) × 10(-6) mol L(-1), and the was in a noncompetitive manner through monophasic process. fluorescence quenching conformational changes determined by circular dichroism were due to formation α-glucosidase-apigenin complex, binding mainly driven hydrophobic interactions hydrogen bonding. molecular simulation bound...

Xanthine oxidase (XO), a key enzyme in purine catabolism, is widely distributed human tissues. It can catalyze xanthine to generate uric acid and cause hyperuricemia gout. Inhibition kinetics assay showed that kaempferol inhibited XO activity reversibly competitive manner. Strong fluorescence quenching conformational changes of were found due the formation kaempferol-XO complex, which was driven mainly by hydrophobic forces. The molecular docking further revealed inserted into cavity...

The binding mechanism of molecular interaction between diosmetin and human serum albumin (HSA) in a pH 7.4 phosphate buffer was studied using atomic force microscopy (AFM) various spectroscopic techniques including fluorescence, resonance light scattering (RLS), UV–vis absorption, circular dichroism (CD), Fourier transform infrared (FT–IR) spectroscopy. Fluorescence data revealed that the fluorescence quenching HSA by static procedure. constants number sites were evaluated at different...

The flavonoid family has been reported to possess a high potential for inhibition of xanthine oxidase (XO). This study concerned the structural aspects inhibitory activities and binding affinities flavonoids as XO inhibitors. result indicated that hydrophobic interaction was important in XO, ability increased generally with increasing within class flavones flavonols. planar structure C2═C3 double bonds were advantageous inhibition. Both hydroxylation on ring B substitution at C3 unfavorable...

Recently, with the advance of deep Convolutional Neural Networks (CNNs), person Re-Identification (Re-ID) has witnessed great success in various applications.However, limited receptive fields CNNs, it is still challenging to extract discriminative representations a global view for persons under non-overlapped cameras.Meanwhile, Transformers demonstrate strong abilities modeling long-range dependencies spatial and sequential data.In this work, we take advantages both CNNs Transformers,...

The interaction between farrerol and calf thymus DNA in a pH 7.4 Tris-HCl buffer was investigated with the use of neutral red (NR) dye as spectral probe by UV-vis absorption, fluorescence, circular dichroism (CD) spectroscopy, well viscosity measurements melting techniques. It found that molecules could intercalate into base pairs evidenced decreases iodide quenching effect single-stranded (ssDNA) effect, induced CD changes, significant increases relative denaturation temperature DNA....

Betulinic acid (BA), an important pentacyclic triterpene widely distributed in many foods, possesses high antidiabetic activity. In this study, BA was found to exhibit stronger inhibition of α-glucosidase than acarbose with IC50 value (1.06 ± 0.02) × 10-5 mol L-1 a mixed-type manner. bound form BA-α-glucosidase complex, resulting more compact structure the enzyme. The obtained concentrations and spectra profiles components resolved by multivariate-curve resolution-alternating least-squares...

The interaction of indigo carmine (IC) with calf thymus DNA in physiological buffer (pH 7.4), using ethidium bromide (EB) dye as a fluorescence probe, was investigated by ultraviolet-visible absorption, fluorescence, and circular dichroism (CD) spectroscopy, coupled viscosity measurements DNA-melting studies. Hypochromicity the absorption spectra IC enhancement polarization were observed addition DNA. Moreover, binding to able decrease iodide single-stranded (ssDNA) quenching effects,...

The binding characteristics of sodium saccharin (SSA), an artificial sweetener, with calf thymus DNA (ctDNA) were investigated by multispectroscopic techniques, chemometrics, and molecular simulation. A combined fluorescence UV-vis spectroscopic data matrix was resolved the multivariate curve resolution-alternating least-squares (MCR-ALS) chemometrics algorithm. MCR-ALS analysis extracted simultaneously concentration profiles spectra for three components (SSA, ctDNA, SSA-ctDNA complex) to...

Morin can inhibit the activity of α-glucosidase and attenuate generation AGEs both related to diabetes mellitus.