A5043943126- Analytical Chemistry and Chromatography
- Chromatography in Natural Products
- Plant chemical constituents analysis
- Plant tissue culture and regeneration
- Molecular Biology Techniques and Applications
- Plant and animal studies
- Plant biochemistry and biosynthesis
- Phytochemical compounds biological activities
- Plant Taxonomy and Phylogenetics
- Insect-Plant Interactions and Control
- Ecology and Conservation Studies
- Ecology and Vegetation Dynamics Studies
- Urban Heat Island Mitigation
- Plant Gene Expression Analysis
- Forest ecology and management
- Flowering Plant Growth and Cultivation
- Climate Change and Health Impacts
- Genetic diversity and population structure
- Urban Green Space and Health
- Protein Interaction Studies and Fluorescence Analysis
- Land Use and Ecosystem Services
- Noise Effects and Management
- Plant Molecular Biology Research
- Natural product bioactivities and synthesis
- Wood Treatment and Properties
South China Agricultural University
2014-2024
Key Laboratory of Guangdong Province
2021-2022
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2014-2020
Hanalei Watershed Hui
2008
Nanchang University
2007
Abstract Background Before studying gene expression of different organisms, it is important to determine the best reference gene. At present, most accurate method detecting quantitative real-time PCR (RT-qPCR). With this method, genes that are stable in biological systems and under conditions can be obtained. Toona ciliata Roem ( T. ). a valuable fast-growing timber specie. In study, 20 were identified using RT-qPCR, as primary prerequisite for future analysis. Four methods, geNorm,...
Neolamarckia cadamba (Roxb.), a close relative of Coffea canephora and Ophiorrhiza pumila, is an important traditional medicine in Southeast Asia. Three major glycosidic monoterpenoid indole alkaloids (MIAs), cadambine its derivatives 3β-isodihydrocadambine 3β-dihydrocadambine, accumulate the bark leaves, exhibit antimalarial, antiproliferative, antioxidant, anticancer anti-inflammatory activities. Here, we report chromosome-scale N. genome, with 744.5 Mb assembled into 22 pseudochromosomes...
Plant tissues contain abundant polysaccharides, phenolic compounds and other metabolites, which makes it difficult to isolate high-quality RNA from them. In addition, Neolamarckia cadamba contains large quantities of components, particularly RNA-binding alkaloids, the isolation even more challenging. Here, we describe a concise efficient method that combines cetyltrimethyl ammonium bromide (CTAB) Kit (Omega) protocols. Gel electrophoresis showed extracted all tissues, using this protocol,...
Neolamarckia cadamba is a fast-growing tropical hardwood tree that used extensively for plywood and pulp production, light furniture fabrication, building materials, as raw material the preparation of certain indigenous medicines. Lack genomic resources hampers progress in molecular breeding genetic improvement this multipurpose species. In study, transcriptome profiling differentiating stems was performed to understand N. xylogenesis. The sequenced using Illumina paired-end sequencing...
Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency segments were evaluated. results showed that time 0.1% mercuric chloride, concentration Plant Preservative Mixture (PPM), pretreatment method, sampling season had significant effects on...
Sequence-related amplified polymorphism (SRAP) markers were used to investigate the genetic diversity among 30 populations of Toona ciliata Roem. sampled from species’ distribution area in China. To analyze SRAP profiles, 1505 primer pairs screened and 24 selected. A total 656 bands ranging 100 1500 bp acquired, these 505 (77%) polymorphic. The information content (PIC) values ranged 0.32 0.45, with an average 0.41. An analysis molecular variance (AMOVA) indicated that most significant...
Purposes of this study are to explore associations between neighborhood landscape elements and public health in a subtropical high-density urban context, elucidate mediating effects physical environmental factors, propose corresponding renewal strategies. Nine sampling sites were selected Guangzhou, China, data collected from 438 participating residents using the MOS item short survey (SF-36 scale). Landscape neighborhoods, including greenness, blue spaces, walking trail systems, squares,...
Zanthoxylum armatum (Z. armatum) is a highly economically important tree that presents special numbing taste. However, the underlying regulatory mechanism of taste remains poorly understood. Thus, elucidation key genes associated with biosynthesis pathways critical for providing genetic information on Z. armatumand breeding high-quality germplasms this species.Here, de novo transcriptome assembly was performed five major organs armatum, including roots, stems, leaf buds, mature leaves and...
The uncertainty about whether, in China, the genus Melia (Meliaceae) consists of one species (M. azedarach Linnaeus) or two and M. toosendan Siebold & Zuccarini) remains to be clarified. Although putative are morphologically distinguishable, genetic evidence supporting their taxonomic separation is lacking. Here, we investigated diversity population structure 31 populations across natural distribution range China. We used sequence-related amplified polymorphism (SRAP) markers obtained...
Urbanization and climate change have been rapidly occurring globally. Evidence-based healthy city development is required to improve living quality mitigate the adverse impact of outdoor neighborhood environment on public health. Taking Guangzhou as an example explore association health preferably offer some implications for better future development, we measured ten environmental factors (temperature (T), wind-chill index (WCI), thermal stress (HSI), relative humidity (RH), average wind...
Abstract In order to explore which ecological strategies the remnant vegetation is taken adapt urbanization, we investigated three kinds of typical (grassland, plantation, and secondary forest) in 16 sample sites along urban–rural gradient city Guangzhou. this study, examined plant species composition, diversity, analyzed seed dominant tree Schima superba ( S. ) forest sites. Those indicators are strongly related with strategies. Eighteen landscape pattern characteristics were determined...
In highly fragmented urban areas, plant diversity of remnant vegetation may depend not only on community structure and topographical factors, but also landscape heterogeneity. Different buffer radius settings can affect the relative importance these factors to diversity. The aim this study was examine heterogeneity, structure, under different radii in biodiversity hotspots. We established 48 plots Guangzhou city, one A 500 m, 1000 2000 m around center each sample plot, 17 heterogeneity...
<italic>Neolamarckia cadamba</italic> is an indigenous, timber-producing tree species in Southern China that plays important role the sustainable development of local forestry industry. However, geographic genetic variation across its natural distribution area has yet to be characterized for best utilization. Here, we report growth and wood properties <italic>N. from 10 provenances represent entire of<italic> N. China. There was significant diameter breast height (DBH), (H), volume (V),...
Abstract Background:Before studying gene expression of different organisms, it is important to determine the best reference gene. At present, most accurate method detecting quantitative real-time PCR (RT-qPCR). With this method, genes that are stable in biological systems and under conditions can be obtained. Toona ciliata Roem ( T. ). a valuable fast-growing timber specie. In study, 20 were identified using RT-qPCR, as primary prerequisite for future analysis. Four methods, geNorm,...
<title>Abstract</title> The full text of this preprint has been withdrawn by the authors while they make corrections to work. Therefore, do not wish work be cited as a reference. Questions should directed corresponding author.
Abstract Neolamarckia cadamba ( N. ) is an evergreen tree species known for its rapid growth, remarkable wood properties, and significant value in medicine, feeding, landscape. In order to clone a individual with excellent genotype, plant regeneration protocol was successfully established leaves wrapped by bud scales as explants. The optimal sterilization method the 0.1% Mercury Chloride (HgCl 2 treatment 1 min before culturing on Murashige Skoog’s medium (MS) supplemented 3.0 mg/L...
Broussonetia papyrifera (L.) L'Hér. ex Vent. has considerable economic and ecological value a long history of use in China. In this paper, root cuttings were used as the material to establish an efficient vegetative propagation B. papyrifera. The results revealed that segments with diameter 1.5~2.0 cm length 20~30 most suitable for shoot regeneration, these had highest adventitious induction rates (93.3%), strongest shoots, multiplication coefficients (7.07). With regard methods burial,...
Toona ciliata is a traditional woody plant that can be used as medicinal material in China. The extracts of its roots, stems, leaves, and flowers all have wide range bioactive compounds. However, T. has been facing an unresolved pest problem caused by Hypsipyla robusta Moore (HRM), which seriously affects growth development. In this study, the expression level TcMYB3 gene reached maximum (28-fold) at 12 h transcriptome sequencing young stems eaten HRM for 0, 3, 12, 21 were performed. A large...
Neolamarckia cadamba (Roxb.) Bosser is a tropical evergreen broadleaf tree species that could play an important role in meeting the increasing demand for wood products. However, multi-level genetic variation and selection efficiency growth traits N. poorly characterized. We therefore investigated of early by monitoring height (HT), diameter at breast (DBH), volume (V) 39 half-sib families from 11 provenances ages 2, 3, 4, 5, 6 years progeny test. Age-related trends rate, parameters...