A5019029226- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- Plant and Fungal Species Descriptions
- Genetic diversity and population structure
- RNA modifications and cancer
- Plant and animal studies
- Ubiquitin and proteasome pathways
- Mosquito-borne diseases and control
- Plant Diversity and Evolution
- Plant-Microbe Interactions and Immunity
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Plant Virus Research Studies
- Cancer Research and Treatments
- Gene expression and cancer classification
- Cancer-related Molecular Pathways
- Hepatitis C virus research
- Plant Parasitism and Resistance
- Hepatitis B Virus Studies
- Microbial Natural Products and Biosynthesis
- Plant tissue culture and regeneration
- Reproductive Biology and Fertility
- Cancer-related molecular mechanisms research
- Ecology and Vegetation Dynamics Studies
Shenzhen Maternity and Child Healthcare Hospital
2025
Northwest A&F University
2023-2025
Air Force Medical University
2012-2025
Chongqing Medical University
2025
The First Hospital of Changsha
2025
Kunming Institute of Botany
2015-2024
Chinese Academy of Sciences
2015-2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
University of Chinese Academy of Sciences
2023-2024
The world's herbaria contain millions of specimens, collected and named by thousands researchers, over hundreds years. However, this treasure has remained largely inaccessible to genetic studies, because both generally limited success DNA extraction the challenges associated with PCR-amplifying highly degraded DNA. In today's next-generation sequencing world, opportunities prospects for historical have changed dramatically, as most NGS methods are actually designed taking short fragmented...
Tight junctions (TJs) play a key role in mediating paracellular ion reabsorption the kidney. The pathway collecting duct of kidney is predominant route for transepithelial chloride that determines extracellular NaCl content and blood pressure. However, molecular mechanisms underlying are not understood. Here we showed mouse cells, claudin-4 functioned as Cl – channel. A positively charged lysine residue at position 65 was critical its anion selectivity. Claudin-4 observed to interact with...
Membrane proteins are generally classified into the following five types: (1) type I membrane proteins, (2) II (3) multipass transmembrane (4) lipid chain-anchored and (5) GPI-anchored proteins. Prediction of protein types has become one growing hot topics in bioinformatics. Currently, we facing two critical challenges this area: first, how to take account extremely complicated sequence-order effects, second, deal with highly uneven sizes subsets a training dataset. In paper, stimulated by...
Camellia, comprising more than 200 species, is a valuable economic commodity due to its enormously popular commercial products: tea leaves, flowers, and high-quality edible oils. It the largest most important genus in family Theaceae. However, phylogenetic resolution of species has proven be difficult. Consequently, interspecies relationships Camellia are still hotly debated. Phylogenomics an attractive avenue that can used reconstruct tree life, especially at low taxonomic levels.Seven...
Solid organ transplant recipients have high risk of lymphomas, including non-Hodgkin lymphoma (NHL) and Hodgkin (HL). A gap in our understanding post-transplant lymphomas involves the spectrum associated risks their many histologic subtypes. We linked nationwide data on solid transplants from US Scientific Registry Transplant Recipients (1987–2008) to 14 state regional cancer registries, yielding 791 281 person-years follow-up for 19 distinct NHL subtypes HL. calculated standardised...
Microarray analysis was performed on RNA isolated from guard cells that were manually dissected leaves of Arabidopsis. By pooling our data with those two earlier studies Arabidopsis cell protoplasts, we provide a robust view the guard-cell transcriptome, which is rich in transcripts for transcription factors, signaling proteins, transporters, and carbohydrate-modifying enzymes. To test hypothesis photosynthesis-derived sugar signals to adjust stomatal opening, determined profile genes...
Motivation Differential co-expression analysis (DCEA) has emerged in recent years as a novel, systematic investigation into gene expression data. While most DCEA studies or tools focus on the relationships among genes, some are developing potentially more promising research domain, differential regulation (DRA). In our previously proposed R package DCGL v1.0, we provided functions to facilitate basic analyses; however, output from v1.0 could not be translated mechanisms straightforward...
The majority of ovarian primordial follicles are preserved in a dormant state to maintain the female reproductive lifespan, and only few activated enter growing follicle pool each wave. Recent studies have shown that follicular activation depends on mammalian target rapamycin complex 1 (mTORC1)‐KIT ligand (KITL) signaling pre‐granulosa cells its receptor (KIT)‐phosphoinositol 3 kinase (PI3K) oocytes. However, upstream regulator mTORC1 is unclear. results present study showed phosphorylated...
Acer yangbiense is a newly described critically endangered endemic maple tree confined to Yangbi County in Yunnan Province Southwest China. It was included programme for rescuing the most threatened species China, focusing on "plant with extremely small populations (PSESP)". We generated 64, 94, and 110 Gb of raw DNA sequences obtained chromosome-level genome assembly A. through combination Pacific Biosciences Single-molecule Real-time, Illumina HiSeq X, Hi-C mapping, respectively. The final...
Significance How the kidney handles chloride reabsorption has long been a mystery. Here, we have discovered pathway in that utilizes channel-activating protease 1 and claudin-4 to physiologically regulate tight junction permeability chloride. Such not only is important renal regulation of but also may play key roles transport across many other epithelia such as lung, salivary gland, skin. This discovery attests concept “druggable” junction. Proteases or protease-dependent mechanisms be...
African swine fever (ASF), caused by virus (ASFV), is a highly contagious disease with high morbidity and mortality in domestic pigs. Although adaptation of ASFV to Vero cells has been investigated, the phenotypic changes corresponding genomic variations during other cell lines remain unclear. To obtain cell-adapted strain, different were tested determine whether they support infection. Interestingly, wild-type strain ASFV-HLJ/18 can infect HEK293T replicate at low level. After continuous...
Inteins are protein-intervening sequences that can self-excise and concomitantly splice together the flanking polypeptides. Two-piece split inteins capable of protein trans-splicing have been found in nature engineered laboratories, but they all a similar site corresponding to endonuclease domain intein. Can be at other sites do trans-splicing? After testing 13 into Ssp DnaB mini-intein, we report finding three new each produced two-piece intein trans-splicing. These functional located...
The aim of this study is to assess the role Frizzled-4 (FZD4) in familial exudative vitreoretinopathy (FEVR) and Coats disease.Tissue samples were collected for DNA extraction automated sequencing two coding exons FZD4 both directions. Cases carrying a mutation demonstrating extreme disease severity selected direct all LRP5, NDP TSPAN12. Clinical data obtained purpose identifying genotype-phenotype correlations.68 probands diagnosed as having autosomal dominant or sporadic FEVR. Eleven...
The molecular nature of tight junction architecture and permeability is a long-standing mystery. Here, by comprehensive biochemical, biophysical, genetic, electron microscopic analyses claudin-16 -19 interactions--two claudins that play key polygenic roles in fatal human renal disease, FHHNC--we found 1) form stable dimer through cis association transmembrane domains 3 4; 2) mutations disrupting the interaction increase ultrastructural complexity but reduce permeability; 3) no claudin...
Significance The role of tight junction permeability in causing human diseases is an important but understudied area. Here, we discovered a previously unidentified mechanism the kidney that uses Kelch-like 3-dependent ubiquitination pathway to regulate claudin-8 and paracellular chloride. importance posttranslational regulation structure function shown here, with emphasis on its potential hypertension if proper disrupted by genetic mutations. Such may also be explored as pharmacologic tool...
The Cornales is the basal lineage of asterids, largest angiosperm clade. Phylogenetic relationships within order were previously not fully resolved. Fifteen plastid genomes representing 14 species, ten genera and seven families newly sequenced for comparative analyses genome features, evolution, phylogenomics based on different partitioning schemes filtering strategies.All plastomes species had typical quadripartite structure with a size ranging from 156,567 bp to 158,715 bp, which included...