A5058343596- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Single-cell and spatial transcriptomics
- Viral Infections and Immunology Research
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- RNA regulation and disease
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- HIV Research and Treatment
- Air Quality and Health Impacts
- MicroRNA in disease regulation
- Cardiac Fibrosis and Remodeling
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Colorectal Cancer Treatments and Studies
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Building Energy and Comfort Optimization
- Biomarkers in Disease Mechanisms
Instituto Gulbenkian de Ciência
2025
University of Lisbon
2023-2024
Spanish National Centre for Cardiovascular Research
2017-2022
La Jolla Institute for Immunology
2022
The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging not universally available). Additional approaches to are desirable. We sought identify a novel microRNA for the myocarditis.To specific myocarditis, we performed microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells type 17 helper (Th17) after inducing experimental autoimmune myocardial infarction...
Relapsed or refractory acute myeloid leukemia (AML) remains a major therapeutic challenge. We have recently developed Vδ1+ γδ T cell-based product for adoptive immunotherapy, named Delta One (DOT) cells, and demonstrated their cytolytic capacity to eliminate AML cell lines primary blasts in vitro vivo. However, the molecular mechanisms responsible broad DOT-cell recognition of cells remain poorly understood. Here, we dissected role natural killer (NK) receptor ligands by DOT cells. Screening...
Increasing evidence has pointed to the important function of T cells in controlling immune homeostasis and pathogenesis after myocardial infarction (MI), although underlying molecular mechanisms remain elusive. In this study, a broad analysis markers 283 patients revealed significant CD69 overexpression on Tregs MI. Our results mice showed that expression increased survival left anterior descending (LAD) coronary artery ligation. Cd69–/– developed strong IL-17+ γδT cell responses ischemia...
Thymus-derived regulatory T (tTreg) cells are key to preventing autoimmune diseases, but the mechanisms involved in their development remain unsolved. Here, we show that C-type lectin receptor CD69 controls tTreg cell and peripheral Treg homeostasis through regulation of BIC/microRNA 155 (miR-155) its target, suppressor cytokine signaling 1 (SOCS-1). Using Foxp3-mRFP/cd69+/- or Foxp3-mRFP/cd69-/- reporter mice short hairpin RNA (shRNA)-mediated silencing miR-155 transfection approaches,...
Air pollutants increase the risk and mortality of myocardial infarction (MI). The aim this study was to assess inflammatory changes in circulating immune cells microRNAs MIs related short-term exposure air pollutants. We studied 192 patients with acute coronary syndromes 57 controls stable angina. For each patient, pollution 24-h before admission, collected. All underwent systematic cell analyses. According PM2.5 exposure, 31 were selected for microRNA STEMI exposed showed a reduction CD4+...
Single-cell RNA-sequencing (scRNA-Seq) is widely used to characterize immune cell populations. However, mRNA levels correlate poorly with expression of surface proteins, which are well established define types. CITE-Seq (cellular indexing transcriptomes and epitopes by sequencing) utilizes oligonucleotide-tagged antibodies simultaneously analyze phenotypes transcriptomes. Considering the high costs adding phenotyping scRNA-Seq, we aimed determine 188 tested can detect their antigens on human...
Abstract Cryopreserved peripheral blood mononuclear cells (PBMCs) are frequently collected and provide disease- treatment-relevant data in clinical studies. Here, we developed combined protein (40 antibodies) transcript single cell (sc)RNA sequencing PBMCs. Among 31 participants the WIHS Study, sequenced 41,611 cells. Using Boolean gating followed by Seurat UMAPs Louvain clustering, identified 58 subsets among CD4 T, CD8 B, NK monocytes. This resolution was superior to flow cytometry, mass...
Abstract CD69 depletion from the lymphoid compartment promotes a Th17/Treg imbalance and exacerbates development of atherosclerosis. As atherosclerosis is trigger myocardial infarction, we have analyzed role in Treg cells after a) permanent occlusion left-anterior-descending coronary artery (LAD-ligation) mice, b) angiography two cohorts acute infarction (MI) patients. Our data show that expression critical to maintain immune homeostasis increases overall survival mice LAD-ligation. Cd69−/−...