A5012115531- Barrier Structure and Function Studies
- Helicobacter pylori-related gastroenterology studies
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Gut microbiota and health
- Immune Cell Function and Interaction
- Cancer Mechanisms and Therapy
- Protein Degradation and Inhibitors
- Microscopic Colitis
- Tissue Engineering and Regenerative Medicine
- CNS Lymphoma Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- Anesthesia and Neurotoxicity Research
- Digestive system and related health
- Epigenetics and DNA Methylation
- Pneumothorax, Barotrauma, Emphysema
- Neurogenetic and Muscular Disorders Research
- Lung Cancer Treatments and Mutations
- Cancer-related cognitive impairment studies
- Mesenchymal stem cell research
- Lymphoma Diagnosis and Treatment
- Electrical and Bioimpedance Tomography
- Histone Deacetylase Inhibitors Research
- Body Composition Measurement Techniques
- Electrospun Nanofibers in Biomedical Applications
University of Maryland, College Park
2020-2025
North Carolina State University
2013-2018
Sungkyunkwan University
2010-2015
Samsung Medical Center
2010-2015
North Central State College
2014
Intestinal anoxia/reoxygenation (A/R) injury induces loss of barrier function followed by epithelial repair. Myosin light chain kinase (MLCK) has been shown to alter via regulation interepithelial tight junctions, but not studied in intestinal A/R injury. We hypothesized that would disrupt junction MLCK activation and myosin (MLC) phosphorylation. Caco-2BBe1 monolayers were subjected anoxia for 2 h reoxygenation 21% O2, after which was determined measuring transepithelial electrical...
Abstract Existing gastrointestinal (GI) diagnostic tools are unable to non-invasively monitor mucosal tight junction integrity in vivo beyond the esophagus. In GI tract, local inflammatory processes induce alterations proteins, enhancing paracellular ion permeability. Although transepithelial electrical resistance (TEER) may be used laboratory assess barrier integrity, there no existing methodologies for characterizing dilation vivo. Addressing this technology gap, intraluminal bioimpedance...
Angiogenesis is one of the most important processes for cancer cell survival, tumor growth and metastasis. Vascular endothelial factor (VEGF) its receptor, particularly VEGF receptor-2 (VEGFR-2, or kinase insert domain-containing KDR), play critical roles in tumor-associated angiogenesis. We developed TTAC-0001, a human monoclonal antibody against VEGFR-2/KDR from fully naïve single-chain variable fragment phage library. TTAC-0001 was selected as lead candidate based on affinity, ligand...
We have previously reported that the ClC-2 chloride channel has an important role in regulation of tight junction barrier function during experimental colitis, and pharmaceutical activator lubiprostone initiates intestinal repair ischemic-injured intestine. Thus, we hypothesized activation would a protective therapeutic effect murine models which be absent mice.We administered to wild-type or mice with dextran sulfate sodium (DSS) 2, 4, 5-trinitrobenzene sulfonic acid-induced colitis....
Vascular endothelial growth factor (VEGF) and its receptors are considered the primary cause of tumor-induced angiogenesis. Specifically, VEGFR-2/kinase insert domain receptor (KDR) is part major signaling pathway that plays a significant role in tumor angiogenesis, which associated with development various types metastasis. In particular, KDR involved angiogenesis as well cancer cell survival. this study, we evaluated therapeutic potential TTAC-0001, fully human antibody against...
Adherens junctions (AJs), together with tight (TJs), form an apical junctional complex that regulates intestinal epithelial cell-to-cell adherence and barrier homeostasis. Within the AJ, membrane-bound E-cadherin binds β-catenin, which functions as essential intracellular signaling molecule. We have previously identified a novel protein in region of junction complex, chloride channel protein-2 (ClC-2), we used to study TJ regulation. In this study, investigated possible effects ClC-2 on...
Intestinal epithelial cell activities during homeostasis and regeneration are well described, but their potential interactions with stromal cells remain unresolved. Exploring the functions of these heterogeneous intestinal mesenchymal (iMSCs) remains challenging due to lack specific markers for most functionally homogenous subpopulations. In recent years, however, novel clustering techniques such as single-cell RNA sequencing (scRNA-seq), fluorescence-activated sorting (FACS), confocal...
Intestinal epithelial cell activities during homeostasis and regeneration are well described, but their potential interactions with stromal cells remain unresolved. Exploring the functions of these heterogeneous intestinal mesenchymal (iMSCs) remains challenging. This difficulty is due to lack specific markers for most functionally homogenous subpopulations. In recent years, however, novel clustering techniques such as single-cell RNA sequencing (scRNA-seq), fluorescence-activated sorting...
The aim of present study is to investigate more functional neural stem cells (NSCs) could be isolated from brains with amyotrophic lateral sclerosis (ALS) and expanded in vitro, based on previous reports demonstrating de novo neurogenesis enhanced replace degenerating tissue.Thirteen- or eighteen-week-old mutant human Cu/Zn superoxide dismutase (SOD1(G93A)) transgenic ALS wild-type SOD1 control mice were utilized. Changes numbers NSCs the dentate gyrus analyzed by immunohistochemistry...
Intraepithelial T lymphocytes (T-IELs), which constitute over 50% of the total in animal, patrol mucosal epithelial lining to defend against pathogen invasion while maintaining gut homeostasis. In addition expressing cell markers such as CD4 and CD8, T-IELs display receptors (TCR), including either TCRαβ or TCRγδ. Both humans mice share similar T-IEL subsets: TCRγδ+, TCRαβ+CD8αα+, TCRαβ+CD4+, TCRαβ+CD8αβ+. Among these subsets, human are predominantly TCRαβ+ (over 80%), whereas those mostly...
We hypothesized that methotrexate (MTX) normalizes the increased permeability of blood-tumor barrier and thus reduces accessibility rituximab (RTX) to central nervous system (CNS) lymphoma. Here, we evaluated combinational treatment capability RTX MTX using an alternative schedule against CNS developed a lymphoma animal model closely mimics morphological molecular characteristics human by injecting Raji Burkitt cells into brains immune-compromised mice tested novel which penetration was not...
Intestinal epithelial cells (IECs) play key roles in nutrient absorption and fluid secretion, providing a barrier to protect cells. IECs, derived from intestinal stem (ISCs), are crucial maintaining homeostasis regeneration, which is gastrointestinal diseases such as inflammatory bowel disease (IBD). To study injury/regeneration, primary cultured colonoids were utilized model system mimic injury. They retain the composition differentiation of tissue, making it suitable for studying...
Lubiprostone, a ClC‐2 chloride channel activator, induces intestinal secretion and is used in the management of idiopathic chronic constipation. Recent studies have suggested that lubiprostone has multiple targets mechanisms action intestine. However, we previously reported initiates barrier repair ischemic‐injured intestine via its principal target, ClC‐2. Thus, hypothesized would protective or therapeutic effect an vivo colitis model. We administrated dextran sulfate sodium (DSS)‐induced...