- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Phytochemicals and Medicinal Plants
- Berberine and alkaloids research
- Alkaloids: synthesis and pharmacology
- Phytochemistry and Biological Activities
- Heavy metals in environment
- Trace Elements in Health
- Medicinal Plants and Neuroprotection
- Essential Oils and Antimicrobial Activity
- Plant biochemistry and biosynthesis
- Chromium effects and bioremediation
- Botanical Research and Chemistry
- Ethnobotanical and Medicinal Plants Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Agricultural pest management studies
- Microbial Natural Products and Biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Agriculture, Plant Science, Crop Management
- Chemical synthesis and alkaloids
- Plant Pathogenic Bacteria Studies
- Phytochemistry and biological activity of medicinal plants
- Composting and Vermicomposting Techniques
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
Central Institute of Medicinal and Aromatic Plants
2005-2024
Indian Council of Medical Research
2023
National Botanical Research Institute
2022
Maharshi Dayanand University
2022
Academy of Scientific and Innovative Research
2019-2021
University of Missouri–Kansas City
2021
Nirma University
2020
Banaras Hindu University
1979-2013
Command Hospital
2011
Institute of Medical Sciences
1998-2004
Abstract Not much is known about the mechanism of endophyte-mediated induction secondary metabolite production in Catharanthus roseus . In present study two fungal endophytes, Curvularia sp. CATDLF5 and Choanephora infundibulifera CATDLF6 were isolated from leaves plant that found to enhance vindoline content by 229–403%. The endophytes did not affect primary metabolism as maximum quantum efficiency PSII, net CO 2 assimilation, biomass starch endophyte-inoculated plants was similar...
Abstract Tissue specific biosynthesis of secondary metabolites is a distinguished feature medicinal plants. Withania somnifera , source pharmaceutically important withanolides biosynthesizes withaferin-A in leaves and withanolide-A roots. To increase the planta production, sustainable approach needs to be explored. Here, we isolated endophytes from different parts W. plants their promising role withanolide was established both in-vivo grown as well in-vitro raised composite Overall, fungal...
The aim of this study was to determine whether ACC (1-aminocyclopropane-1-carboxylic acid) deaminase-containing bacterial treatments could enhance the tolerance poppy (Papaver somniferum L.) plants against biotic stress downy mildew caused by Peronospora sp.Three different genotypes P. somniferum, that is, Sampada, J-16 and I-14 were included in experiment. bacteria Pseudomonas putida (WPTe) reduced disease severity significantly improved growth yield plants. chlorophyll content,...
Introduction The exact role of copper and zinc in the etiology carcinoma gallbladder is unclear. Some studies suggest Cu/Zn ratio a good indicator extent prognosis gastrointestinal tract. aim present study to estimate micronutrient profile serum, tissues, bile patients with benign malignant diseases. Methods was carried out 60 comprising 30 each cholelithiasis, age sex matched controls. Copper levels were estimated blood, bile, tissue using Perkin Elmer Model 2380 Atomic absorption...
The aim of this study was to identify and characterize promising endophytes capable enhancing the content root alkaloids ajmalicine serpentine in low alkaloid yielding genotype Catharanthus roseus cultivar Prabal possible mechanisms involved.Of four strains isolated from alkaloid-rich C. Dhawal, endophytic CATDLF5 (Curvularia sp.) CATDLF6 (Choanephora infundibulifera) enhanced by 211·7-337·6%, while CATDRF2 (Aspergillus japonicus) CATDS5 (Pseudomonas increased 123·4-203·8% Prabal....
Aims: To investigate intracellular localization of nickel and its chemical nature in Pseudomonas aeruginosa. Methods Results: Transmission electron micrographs Ni‐loaded bacteria exhibited a darkened opaque zone throughout the cell periphery. Energy dispersive X‐ray analysis confirmed deposition metallic nickel. Cell fractionation revealed that 88% accumulated was restricted to periplasm membrane. diffraction patterns ascertained cellular Ni as phosphide (Ni5P4, NiP2 Ni12P5) carbide (Ni3C)...
Carcinoma of the gall bladder is third most common malignancy gastrointestinal tract in eastern Uttar Pradesh and western Bihar regions India.1 The two lie down stream river Ganges, which main source drinking, bathing, irrigation water this part India receives untreated domestic sewage industrial agricultural effluent. High concentrations cadmium have been reported sewage, water, vegetables grown area, higher heavy metals than recommended by World Health Organisation from region. Heavy as...
A simple reversed-phase liquid chromatographic method is developed for the simultaneous quantitation of anticancerous drugs vincristine, vinblastine, and their precursors catharanthine vindoline using a Merck Chromolith Performance high-performance chromatography column. better resolution obtained in comparison with available particulate-type C18 columns. The column provides good reproducibility peak symmetry. Chromatography carried isocratically mobile phase acetonitrile-0.1M phosphate...
Carcinoma of the gallbladder is third most common malignancy gastrointestinal tract in Eastern Uttar Pradesh and Western Bihar regions India. The main source drinking water this region river Ganges, which heavily polluted with agricultural pesticides. Organochlorine pesticides were estimated bile by gas liquid chromatography 60 patients (30 carcinoma 30 cholelithiasis) to observe its association aetiopathogenesis gallbladder. mean biliary concentration benzene hexachloride (BHC) was found be...
Abstract Comparative study on the essential oil constituents of Pogostemon cablin (Blanco) Benth. (Patchouli) and P. travancoricus Bedd. var. were investigated using GC GC/MS analysis. Eleven compounds from 13 identified. Both species shared like α- β-patchoulene, patchouli alcohol (patchoulol), β-caryophyllene, α-guaiene, seychellene selinene, although quantitatively less in travancoricus.
Papaver somniferum L. produces therapeutically-useful alkaloids like morphine, codeine, papaverine, and thebaine that accumulate in the latex of its capsule. Apart from alkaloids, edible seeds plant have high nutritional value culinary use, with seed oil being rich health-promoting unsaturated fatty acids. Sujata is low alkaloid-producing latex-less variety P. developed an alkaloid-rich gum harvest parent (Sampada) for curtailing narcotic menace morphine opium (dried latex). Earlier, it has...
Abstract A reversed‐phase column liquid chromatographic method for the analysis of sennosides and B present in leaf pod extracts Cassia angustifolia has been developed using a Symmetry C 18 linear binary gradient profile. The can be utilised quantitative determination other as baseline resolution most constituents was achieved. is economical terms time taken amount solvent used (25 mL) each analysis. validity with respect to confirmed by comparing UV spectra peak those reference compounds...
A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of medicinally useful laxative principles sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved recoveries 97 96% respectively.