A5090797998- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Cytokine Signaling Pathways and Interactions
- Phagocytosis and Immune Regulation
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Fibroblast Growth Factor Research
- Nanoparticle-Based Drug Delivery
- Hepatitis C virus research
- Pancreatic function and diabetes
- Organ Transplantation Techniques and Outcomes
- Liver Diseases and Immunity
- Prostate Cancer Treatment and Research
- Sphingolipid Metabolism and Signaling
- Pediatric Hepatobiliary Diseases and Treatments
- Obstructive Sleep Apnea Research
- Paraoxonase enzyme and polymorphisms
- Macrophage Migration Inhibitory Factor
- Chemokine receptors and signaling
- Lung Cancer Treatments and Mutations
- Endoplasmic Reticulum Stress and Disease
- Drug-Induced Hepatotoxicity and Protection
- Wnt/β-catenin signaling in development and cancer
University of Twente
2016-2025
Central University of Haryana
2025
European University
2024
Regional Medical Center
2021
RWTH Aachen University
2021
University of Groningen
2009-2021
NewYork–Presbyterian Brooklyn Methodist Hospital
2012-2015
Karolinska Institutet
2012-2015
Target (United States)
2011-2013
Karolinska University Hospital
2013
Abstract Glioblastoma‐associated macrophages (GAMs) play a crucial role in the progression and invasiveness of glioblastoma multiforme (GBM); however, exact crosstalk between GAMs cells is not fully understood. Furthermore, there lack relevant vitro models to mimic their interactions. Here, novel 3D‐bioprinted mini‐brains consisting are presented as tool study interactions these two cell types test therapeutics that target this interaction. It demonstrated mini‐brains, actively recruit...
Tumor-associated macrophages (TAMs) are the key effector cells in tumor microenvironment and induce neoangiogenesis, matrix remodeling, metastasis while suppressing immune system. These protumoral display an M2 phenotype induced by IL-4 IL-13 cytokines. In this study, we hypothesized that inhibition of signal transducer activator transcription 6 (Stat6) pathway, a common downstream signaling pathway IL-13, may be interesting strategy which to inhibit TAM differentiation and, thus, their...
Cancer-associated fibroblasts (CAFs), are the key effector cells in pancreatic ductal adenocarcinoma (PDAC), known to induce tumor growth and progression. Pancreatic stellate (PSCs) precursors of CAFs PDAC that secrete abundant extracellular matrix, factors cytokines. In this study, we targeted human relaxin-2 (RLX), an endogenous hormone, PSCs inhibit their differentiation into CAF-like myofibroblasts. RLX significantly inhibited TGF-β induced by inhibiting pSmad2 signaling pathway. vitro...
Abundant desmoplastic stroma is the hallmark for pancreatic ductal adenocarcinoma (PDAC), which not only aggravates tumor growth but also prevents penetration of chemotherapy, leading to treatment failure. There an unmet clinical need develop therapeutic solutions problem. In this study, we investigated potential integrin α5 (ITGA5) receptor in PDAC stroma. ITGA5 was overexpressed from patient samples, and overexpression inversely correlated with overall survival. vitro, knockdown inhibited...
Liver fibrogenesis is a process tightly controlled by endogenous anti- and pro-fibrogenic factors. Interferon gamma (IFNγ) potent antifibrogenic cytokine in vitro might therefore represent powerful therapeutic entity. However, its poor pharmacokinetics adverse effects, due to the presence of IFNγ receptors on nearly all cells, prevented clinical application so far. We hypothesized that delivery specifically disease-inducing cells concurrently avoiding binding nontarget increase efficacy...
Quiescent hepatic stellate cells (HSCs), in response to liver injury, undergo characteristic morphological transformation into proliferative, contractile and ECM-producing myofibroblasts. In this study, we investigated the implication of canonical Wnt signaling pathway HSCs fibrogenesis. Canonical activation inhibition using β-catenin/CBP inhibitor ICG001 was examined in-vitro TGFβ-activated 3T3, LX2, primary human HSCs, in-vivo CCl4-induced acute injury mouse model. Fibroblasts-conditioned...
Abstract Hepatic stellate cells (HSCs) known as “master producers” and macrophages regulators”, are the key cell types that strongly contribute to progression of liver fibrosis. Since Notch signaling regulates multiple cellular processes, we aimed study role in HSCs differentiation polarization evaluate its implication fibrogenesis. pathway components were found be significantly upregulated TGFβ-activated HSCs, inflammatory M1 mouse human fibrotic livers. Interestingly, inhibition using a...
Renal fibrosis leads to end-stage renal disease demanding replacement therapy because no adequate treatment exists. IFN-γ is an antifibrotic cytokine that may attenuate fibrosis. Systemically administered causes side effects be prevented by specific drug targeting. Interstitial myofibroblasts are the effector cells in fibrogenesis. Here, we tested hypothesis cell-specific delivery of platelet-derived growth factor receptor β (PDGFRβ)-expressing attenuates obstructive nephropathy [unilateral...
Tissue fibrosis, characterized by excessive accumulation of aberrant extracellular matrix (ECM) produced myofibroblasts, is a growing cause mortality worldwide. Understanding the factors that induce myofibroblastic differentiation paramount to prevent or reverse fibrogenic process. Integrin-mediated interaction between ECM and cytoskeleton promotes myofibroblast differentiation. In present study, we explored significance integrin alpha 11 (ITGA11), subunit selectively binds type I collagen...
Pancreatic ductal adenocarcinoma (PDAC) is the deadliest tumor due to its highly abundant stroma.Pancreatic stellate cells (PSCs) are considered precursor of cancer-associated fibroblasts (CAFs), which induce progression, invasion, and metastasis.In this study, we investigated role integrin subunit a (ITGA) 11, receptor for collagen type I, in stroma interaction.Clinical sample analysis showed that ITGA11 was overexpressed by CAFs PDAC stroma, as shown with colocalization immunostaining...
Liver diseases are the growing health problem with no clinically approved therapy available. Activated hepatic stellate cells (HSCs) key driver responsible for extracellular matrix deposition, hallmark of liver fibrosis. Fibroblast growth factor 2 (FGF2) has shown to possess anti-fibrotic effects in fibrotic including fibosis, and promote tissue regeneration. Among fibroblast receptors (FGFRs), FGF2 interact primarily FGFR1, highly overexpressed on activated HSCs, inhibit HSCs activation....
Liver fibrosis affects millions of people worldwide and is rising vastly over the past decades. With no viable therapies available, liver transplantation only curative treatment for advanced diseased patients. Excessive accumulation aberrant extracellular matrix (ECM) proteins, mostly collagens, produced by activated hepatic stellate cells (HSCs), a hallmark fibrosis. Several studies have suggested an inverse correlation between collagen-I degrading metalloproteinase-1 (MMP-1) serum levels...
Abstract Hepatic fibrosis, a progressive chronic disease mainly caused by hepatitis viral infections, alcohol abuse or metabolic syndrome leading to liver dysfunction and is the growing cause of mortality worldwide. Tyrosine kinase inhibitor BIBF1120 (Nintedanib) has been evaluated in clinical trials for idiopathic pulmonary fibrosis advanced Hepatocellular carcinoma, but not explored yet. In this study, we aimed investigate therapeutic effects mechanism fibrogenesis. The were TGFβ-activated...
Abstract Drug diffusion within the skin with a needle-free micro-jet injection (NFI) device was compared two well-established delivery methods: topical application and solid needle injection. A permanent make-up (PMU) machine, normally used for dermal pigmentation, utilized as method. For NFIs continuous wave (CW) laser diode to create bubble inside microfluidic containing light absorbing solution. Each method delivered different solutions into ex vivo porcine skin. The first solution...
Non-alcoholic steatohepatitis (NASH) is the leading cause of cirrhosis worldwide and most rapidly growing indication for liver transplantation. Macrophages are important cellular component in inflammatory milieu NASH. Inflammatory pro-fibrotic mediators produced by macrophages causes significant tissue injury many diseases. Therefore, inhibition would be a promising approach to attenuate In this study, we studied implication SYK pathway NASH, investigated PLGA nanoparticles-based delivery...