Abstract We analyzed the potential antibacterial effects of two different PdB against methicillin-resistant S. aureus and P. aeruginosa . The third-degree burn wound healing was also studied. Blood samples were obtained from 10 healthy volunteers biological assays performed antimicrobial activity MRSA determined using disk diffusion (DD), broth microdilution (BMD), time-kill assay methods. 48 Wistar albino rats burned infected with MRSA. Two groups injected PdB, control treated plasma...

Effects of the Aqueous Extract Aloe vera on Morphological and Physiological Properties E. coli ________________________________________ Mahdi Kargaran 1 ; Ali Reza Moradabadi Mohammad Arjomandzadegan 1, * Hossein Hosseini Ghasem Habibi Maryam Tayeboon Hadi Karami Ahmad Akbari 1 Tuberculosis Pediatric Infectious Research Center, Department Microbiology, Arak University Medical Sciences, Arak, IR Iran *Corresponding Authors: Arjomandzadegan, Iran, E-mail: arjomandzadegan@arakmu.ac.ir;...

Abstract Background and aim Treatment of burn wound infections has become a global challenge due to the spread multidrug-resistant bacteria; therefore, development new treatment options for mentioned is essential. Platelets have drawn much attention this purpose because they are safe cost-effective source different antimicrobial peptides growth factors. The present study evaluated antibacterial effects healing properties Platelet-derived Biomaterial (PdB) against Acinetobacter baumannii...

Acinetobacter Baumannii is an opportunistic nosocomial pathogen belonging to the Moraxellaceae family. The emergence of multidrug resistant strains this caused many problems for hospitals and patients. aim current study was isolate, identify, morphologically, physiologically, in vivo analyze a new lytic bacteriophage targeting extensively drug-resistant (XDR) A. baumannii.Different wastewater samples were tested isolation against 19 baumannii isolates obtained from patients hospitalized...

Chronic lymphocytic leukemia (CLL) is one of the most common hematologic malignancy in adults worldwide. This cancer has a poor prognosis at different stages. So, identification new biomarkers important for diagnosis B-CLL. Considering oncogenic role APRIL molecule this as well regulatory miRNAs signaling pathways, present study evaluated targeting gene

Myeloproliferative neoplasms (MPN) are heterogeneous diseases that classified by the presence of Philadelphia chromosome into negative (Ph-neg) and positive (Ph-pos) myeloproliferative neoplasms. In ph-neg group A somatic point mutation (c.1849G>T) in JAK2 gene, part JAK2-STAT signal-transduction pathway, causes substitution phenylalanine for valine (V617F) protein has been identified. This was seen PV 65% to 97% ET (30-57%) primary myelofibrosis (35-95%). Highly sensitive methods have used...

The Janus kinase 2 (JAK2) has an important role in the intracellular signaling normal and neoplastic cells. JAK2 mutation, called V617F, is frequently found Philadelphia chromosome-negative myeloproliferative neoplasms. We aimed to assess analytical efficiency of high-resolution melting (HRM) method using reannealing-curve analysis comparison with routine melting-curve for V617F mutation detection.

Abstract Objective Histopathological studies suggest that parasite load is different between acute and chronic forms of cutaneous leishmaniasis (CL). However, highly sensitive detection methods are still needed to distinguish leishmaniasis. In the present study, we developed a quantitative real-time polymerase chain reaction (PCR) detect quantify Leishmania tropica parasites in paraffin-embedded tissue samples. Results The ability PCR for leishmania was higher than histopathological...

Acute myeloid leukemia is caused by the clonal proliferation of undifferentiated hematopoietic precursors. AML prognosis highly involved in treatment response and determined mutations several genes such as N-RAS. This study aims to identify distribution common N-RAS (codons 12, 13, 61) patients using HRM method confirm this method's efficiency for mutation detection comparing its results with sequencing data Gold standard method.

Abstract Objective The resistance to antimony-containing glucantime is a major obstacle successful treatment, especially in endemic areas. Looking the molecular mechanisms involved this drug will help choosing best treatment. aim of study was evaluate expression multidrug-resistance 1 (MDR1) and protein A (MRPA) genes acute, chronic non-lupoid, lupoid forms dry type cutaneous leishmaniasis (DTCL). Results MDR1 gene over-expressed as 14.4- 1.56-folds acute compared with non-lupoid form,...

Chronic lymphocytic leukemia (CLL) is one of the most prevalent forms in adults. Inactivation DLEU7 gene frequently observed patients with CLL. Furthermore, microRNAs (miRNAs) have been to a critical role pathogenesis several cancers, including leukemia. Considering tumor-suppressive DLEU7, as well tumor suppressor or oncogenic (miRNAs), aim present study was evaluate potential miRNAs targeting B-cells and explore expression changes these genes plasma B-CLL patients.The interacting were...

The chemokine-receptor axes play parts in development of leukemia, CXCL1, CXCL10 and CXCL12 are involved immune responses. Thus, we have examined the serum levels these chemokines parallel with their related cognate receptors (CXCR1, CXCR3 CXCR4) AML (acute myeloid leukemia) patients prior post BMT (bone marrow transplantation) therapy.Clinical specimens were collected from 46 (23 M1 23 M3 subtypes) before/after BMT. concentrations determined by ELISA. mRNA detected QRT_PCR. Data analyzed...

Acute promyelocytic leukemia is the most prevalent AML malignancy. The side effects caused by chemotherapeutic drugs have primarily led to increased use of natural products for cancer treatment. Gaillardin, a medicinal herb, has been reported anti-proliferative on various cells. In this study, cytotoxic and apoptotic Gaillardin were investigated acute cell lines, NB4. Different concentrations used treat NB4 cells 48 72 hours. Then viability was assessed using MTT assay. Flow cytometry...

This study investigated the effect of ethanol extracts horsetail, alfalfa, ortie, chêne and aleppo oak on blood coagulation in vitro. Extraction was performed by maceration method. Extracts were mixed with platelet plasma, then prothrombin time (PT), activated partial thromboplastin (APTT) aggregation tests conducted. Alfalfa extract had a dose-dependent PT. Ortie, reduced PT significantly compared to control group. Alfalfa, ortie APTT, but their insignificant The pooled showed highest...

Thalassemia is a hypochromic microcytic anemia, which characterized by congenital disorders. In thalassemia patients, bone diseases are one of the causes mortality. Our goal was to investigate association between vitamin D deficiency and increased iron uptake cardiac myocytes hepatocytes.Forty patients with major were studied in Amir Kabir Hospital, Arak, Iran. The information obtained through clinical examination. Serum ferritin level determined ELISA T2*MRI performed for measuring content...