A5010672169- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Genetic diversity and population structure
- Plant and animal studies
- Sperm and Testicular Function
- Lepidoptera: Biology and Taxonomy
- Invertebrate Immune Response Mechanisms
- Wildlife Ecology and Conservation
- Physiological and biochemical adaptations
- Plant Reproductive Biology
- Neurobiology and Insect Physiology Research
- Identification and Quantification in Food
- Genetic and phenotypic traits in livestock
- T-cell and Retrovirus Studies
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
University of Oslo
2022-2023
Syracuse University
2015-2022
National Heart Lung and Blood Institute
2022
University of Washington
2022
New York University
2022
University of Southern California
2022
SignificanceIn species with internal fertilization, sperm spend an important part of their lives within the female. To examine life history during this time, we used semiquantitative proteomics and sex-specific isotopic labeling in fruit flies to determine extent molecular continuity between male female reproductive tracts provide a global catalog sperm-associated proteins. Multiple seminal fluid proteins associate immediately after mating. Few remain long-term storage, whereas...
Seminal fluid proteins (SFPs) mediate an array of postmating reproductive processes that influence fertilization and fertility. As such, it is widely held SFPs may contribute to postmating, prezygotic barriers between closely related taxa. We investigated seminal (SF) diversification in a recently diverged passerine species pair (Passer domesticus Passer hispaniolensis) using combination proteomic comparative evolutionary genomic approaches. First, we characterized compared the SF proteome...
Rapid evolution is a hallmark of reproductive genetic systems and arises through the combined processes sequence divergence, gene gain loss, changes in protein expression. While studies aiming to disentangle molecular ramifications these are progressing, we still know little about basis evolutionary transitions systems. Here conduct first comparative analysis sperm proteomes Lepidoptera, group that exhibits dichotomous spermatogenesis, which males produce functional fertilization-competent...
Fertility depends on the progression of complex and coordinated postmating processes within extracellular environment female reproductive tract (FRT). Molecular interactions between ejaculate FRT proteins regulate many these processes, including sperm motility, migration, storage, modification, along with concurrent changes in female. Although extensive progress has been made proteomic characterization male-derived components seminal fluid, investigations into have remained more limited. To...
Abstract Spermatozoa are one of the most strikingly diverse animal cell types. One poorly understood example this diversity is sperm heteromorphism, where males produce multiple distinct morphs in a single ejaculate. Typically, only morph capable fertilization and function nonfertilizing morph, called parasperm, remains to be elucidated. Sperm heteromorphism has independent origins, including Lepidoptera (moths butterflies), fertilizing eupyrene an apyrene which lacks nucleus nuclear DNA....
Abstract Sperm cells are variable both within and among species. To be able to accurately measure sperm understand their function, it is important that preserved in a manner maintains structural integrity. Formalin widely used fixative storage medium for cells, but few studies have examined the effect of fixation long-term on morphological Ethanol also common agent tissue samples, here we examine if formalin or ethanol alters cell size We found no significant effects process fresh fixed...
Abstract Background Rapid evolution is a hallmark of reproductive genetic systems and arises through the combined processes sequence divergence, gene gain loss, changes in protein expression. While studies aiming to disentangle molecular ramifications these are progressing, we still know little about basis evolutionary transitions systems. Here conduct first comparative analysis sperm proteomes Lepidoptera, group that broadly exhibits dichotomous spermatogenesis, which males simultaneously...
Abstract Spermatozoa are one of the most strikingly diverse animal cell types. One poorly understood example this diversity is sperm heteromorphism, where males produce multiple distinct morphs in a single ejaculate. Typically, only morph capable fertilization and function non-fertilizing morph, called parasperm, remains to be elucidated. Sperm heteromorphism has independent origins, including Lepidoptera (moths butterflies), fertilizing eupyrene an apyrene which lacks nucleus nuclear DNA....