A5073885248- Lipid Membrane Structure and Behavior
- Insect and Pesticide Research
- Insect and Arachnid Ecology and Behavior
- Vehicle Routing Optimization Methods
- Infant Nutrition and Health
- Plant and animal studies
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- 14-3-3 protein interactions
- Ubiquitin and proteasome pathways
- Enzyme Structure and Function
- Outsourcing and Supply Chain Management
- Mitochondrial Function and Pathology
- Protein Interaction Studies and Fluorescence Analysis
- Proteins in Food Systems
- Epigenetics and DNA Methylation
- Risk and Safety Analysis
- Cancer-related gene regulation
- Neonatal Health and Biochemistry
- RNA modifications and cancer
- Nanocluster Synthesis and Applications
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
University of Bergen
2016-2025
Norwegian University of Life Sciences
2011-2024
Osnabrück University
2024
Universidade Nova de Lisboa
2024
University of Oslo
2024
Arizona State University
2024
Molde University College
2001-2015
Norwegian University of Science and Technology
2007-2009
SINTEF
2009
The polar bear ( Ursus maritimus ) is the only Arctic land mammal that dives into water to hunt. Despite thermal insulation provided by blubber and fur layers low temperatures, their typically observed be free of ice. This study investigates anti-icing properties fur. Here, we show exhibits ice adhesion strengths comparable fluorocarbon-coated fibers, with a consequence sebum (hair grease). Lipid analyses reveal presence cholesterol, diacylglycerols, anteisomethyl-branched fatty acids,...
The adsorption of eight different proteins (alpha-lactalbumin (types I and III), bovine serum albumin, hemoglobin, myoglobin, cytochrome c, alpha-casein, lysozyme) onto a model anionic surface was performed at equivalent bulk (solvent, ionic strength, pH) conditions. Adsorption monitored on quartz crystal microbalance with dissipation monitoring (QCM-D) citrate-coated gold surfaces as adsorbents has been correlated to native fold stability determined from near- far-UV circular dichroism (CD)...
Purpose In the Norwegian oil and gas industry upstream logistics includes providing offshore installations with needed supplies return flow of used materials equipment. This paper considers a real‐life routing problem for supply vessels serving at Haltenbanken off northwest coast Norway from its onshore base. The purpose is to explore how installation's limited storage capacity affects aiming towards creating efficient routes. Design/methodology/approach A simplified version one vessel...
Protein folding barriers, which range from zero to the tens of RT that result in classical two-state kinetics, are primarily determined by protein size and structural topology [Plaxco KW, Simons KT, Baker D (1998) J Mol Biol 277:985–994]. Here, we investigate thermodynamic barriers two relatively large proteins same topology: bovine α-lactalbumin (BLA) hen-egg-white lysozyme (HEWL). From analysis differential scanning calorimetry experiments with variable-barrier model [Muñoz V, Sanchez-Ruiz...
Exosomes are vesicles involved in intercellular communication. Their membrane structure and core content is largely dependent on the cell of origin. have been investigated both for their biological roles possible use as disease biomarkers drug carriers. These potential technological applications require rigorous characterization exosomal blood brain barrier permeability a description lipid bilayer composition. To achieve these goals, we established 3D static system based existing systems...
Background Cell membrane interactions rely on lipid bilayer constituents and molecules inserted within the membrane, including specific receptors. HAMLET (human α-lactalbumin made lethal to tumor cells) is a tumoricidal complex of partially unfolded (HLA) oleic acid that internalized by cells, suggesting with phospholipid and/or receptors may be essential for effect. This study examined whether interacts artificial membranes alters structure. Methodology/Principal Findings We show surface...
Dysregulation of the biosynthesis cholesterol and other lipids has been implicated in many neurological diseases, including Parkinson's disease. Misfolding α-synuclein (α-Syn), main actor disease, is associated with changes a lipid environment. However, exact molecular mechanisms underlying effect on α-Syn binding to as well oligomerization fibrillation remain elusive, does relative importance compared factors. We probed interactions behaviour using styrene-maleic acid nanodiscs, containing...
Thermophiles, mesophiles, and psychrophiles have different amino acid frequencies in their proteins, probably because of the way species adapt to very temperatures environment. In this paper, we analyse how contacts between sidechains vary homologous proteins from that are adapted temperatures, but displaying relatively high sequence similarity. We investigate whether specific acids is a key factor thermostabilisation proteins. The dataset was divided into two subsets with optimal growth...
For studies of protein−lipid interactions, thin films at the air−water surface are often employed as model systems for cell membranes. A convenient manner in which to study these interactions is Langmuir technique, allows formation monolayer phospholipid together with a choice where and how introduce proteins, according desired response variable. Here, distinction has been made between different interaction protocols it also commented upon what extent introduction protein solution prior...
Vitellogenin, an egg-yolk protein precursor common to oviparous animals, is found abundantly in honeybee workers - a caste of helpers that do not usually lay eggs. Instead, vitellogenin (180 kDa) participates processes other than reproduction: it influences hormone signaling, food-related behavior, immunity, stress resistance and longevity. The molecular basis these functions largely unknown. Here, we establish compare the properties from hemolymph (blood) abdominal fat body, two...
To characterize the interaction of peripheral proteins and membranes at molecular level, we studied reversible association bovine α-lactalbumin (BLA) with lipid bilayers composed different forms phosphatidylserine or equimolar mixtures these egg yolk phosphatidylcholine. At pH 4.5, almost all BLA (>90%) associates to negatively charged small unilamellar vesicles. The conformational changes that binding induced on protein were characterized by circular dichroism fluorescence spectroscopy....
Tyrosine hydroxylase (TH), the rate-limiting enzyme in synthesis of catecholamines, is activated by phosphorylation-dependent binding to 14-3-3 proteins. The N-terminal domain TH also involved interaction with lipid membranes. We investigated its different partners, both unphosphorylated (TH-(1–43)) and Ser19-phosphorylated (THp-(1–43)) states surface plasmon resonance. THp-(1–43) showed high affinity for proteins (Kd ∼ 0.5 μm 14-3-3γ -ζ 7 14-3-3η). domains bind negatively charged membranes...
Small noble metal nanoclusters can be formed in situ by direct reduction and stabilization of a precursor biomolecules such as proteins. Considering the diversity amino acid composition proteins, hence their reductive ability, general method for synthesis gold using proteins is presented here. A range (bovine serum albumin, fibrinogen, α-lactalbumin, lysozyme, cytochrome c, myoglobin, β-lactoglobulin α-chymotrypsin) have been studied, based on size, isoelectric point, flexibility...
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in synthesis of catecholamine neurotransmitters, and a reduction TH activity is associated with several neurological diseases. Human regulated, among other mechanisms, by Ser19-phosphorylation-dependent interaction 14-3-3 proteins. The N-terminal sequence (residues 1–43), which corresponds to an extension regulatory domain, also interacts negatively charged membranes. By using X-ray crystallography together molecular dynamics...
This paper reports that the abundances of endogenous cardiolipin and phosphatidylethanolamine halve during elongation Gram-positive bacterium Listeria innocua. The lyotropic phase behaviour model lipid systems describe these modulations in composition indicate average stored curvature elastic stress membrane is reduced on cell, while fluidity appears to be maintained. These findings suggest phospholipid metabolism linked cell cycle changes can facilitate passage succeding stage cycle....
Protein-stabilized gold nanoconstructs are widely studied due to their potential applications in biosensing, drug and gene delivery, bioimaging. While a number of studies have focused on the novel properties such materials emanating from gold, there has been little focus how protein shell is affected by nanocluster formation with respect conformation, stability function. Herein, we show synthesis protein-stabilized varying size small clusters (~8 Au atoms) dispersed within proteins...