A5017880674- Metabolism and Genetic Disorders
- Protein Structure and Dynamics
- Ubiquitin and proteasome pathways
- Biochemical and Molecular Research
- Spectroscopy and Quantum Chemical Studies
- Computational Drug Discovery Methods
- X-ray Diffraction in Crystallography
- Microbial Natural Products and Biosynthesis
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Crystallization and Solubility Studies
- Seaweed-derived Bioactive Compounds
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Protein Hydrolysis and Bioactive Peptides
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Infant Nutrition and Health
- Meat and Animal Product Quality
- Algal biology and biofuel production
- Neonatal Health and Biochemistry
- Advanced NMR Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Endoplasmic Reticulum Stress and Disease
- Proteins in Food Systems
University of Bergen
2015-2025
Johannes Gutenberg University Mainz
2021
Indian Institute of Chemical Technology
2021
Aarhus University
2006-2015
University of Illinois Urbana-Champaign
2009
Le Mans Université
2009
This case study introduces a green, 1 h single-step method using water-rich natural deep eutectic solvent (WRNADES) for ultrasound-assisted extraction (UAE) of polyphenols from
Protein folding barriers, which range from zero to the tens of RT that result in classical two-state kinetics, are primarily determined by protein size and structural topology [Plaxco KW, Simons KT, Baker D (1998) J Mol Biol 277:985–994]. Here, we investigate thermodynamic barriers two relatively large proteins same topology: bovine α-lactalbumin (BLA) hen-egg-white lysozyme (HEWL). From analysis differential scanning calorimetry experiments with variable-barrier model [Muñoz V, Sanchez-Ruiz...
In this case study, we explored quantitative 1H NMR (qNMR), HPLC-DAD, and the Folin-Ciocalteu assay (TPC) as methods of quantifying total phenolic content a green macroalga, Ulva intestinalis, after optimized accelerated solvent extraction. Tentative qualitative data was also acquired multiple steps purification. The observed polyphenolic profile complex with low individual concentrations. qNMR method yielded 5.5% (DW) polyphenols in crude extract, whereas HPLC-DAD TPC 1.1% 0.4%...
Detailed insight into the interplay between antimicrobial peptides and biological membranes is fundamental to our understanding of mechanism bacterial ion channels action these in host-defense systems. To explore this interplay, we have studied incorporation, membrane-bound structure, conformation peptide alamethicin lipid bilayers using a combination 1H liquid-state NMR spectroscopy molecular dynamics (MD) simulations. On basis experimental data, evaluate simple in-plane transmembrane...
Mutations in the human TGFBI gene encoding TGFBIp have been linked to protein deposits cornea leading visual impairment. The consists of an N-terminal Cys-rich EMI domain and four consecutive fasciclin 1 (FAS1) domains. We compared stabilities wild-type (WT) six mutants known produce phenotypically distinct cornea. Amino acid substitutions first FAS1 (FAS1-1) (R124H, R124L, R124C) did not alter stability. However, within fourth (FAS1-4) (A546T, R555Q, R555W) affected overall stability intact...
The congenital disorder of glycosylation (CDG) due to phosphomannomutase 2 deficiency (PMM2-CDG), the most common N-glycosylation disorder, is a multisystem disease for which no effective treatment available. recent functional characterization disease-causing mutations described in patients with PMM2-CDG led idea therapeutic strategy involving pharmacological chaperones (PC) rescue PMM2 loss-of-function mutations. present work describes high-throughput screening, by differential scanning...
Sustainable production based on renewable biomass and efficient bioprocesses are important elements in the growing blue bioeconomy. The traditional Laminaria hyperborea alginate disposes approximately 80 % of raw material, ignoring large amounts potential high-value products from alga. Particularly, leaf fraction seaweed is often disregarded. This study aimed to characterize high value side-stream – focusing particularly targeting phenolic content. After extraction solvent optimization, 60...
Abstract Precise, fast, and reliable identification quantification of microplastic contamination are essential for determining their environmental concentrations risk assessments. This study investigates the use nuclear magnetic resonance (NMR) spectroscopy to quantify microplastics by analysing dilution series polystyrene (PS), polyisoprene-cis (PI), polybutadiene-cis (PB), polylactic acid (PLA), polyvinyl chloride (PVC) polyurethane (PU). Each polymer type was dissolved in a suitable...
Methylmalonic aciduria (MMA) cblB type is caused by mutations in the MMAB gene. This encodes enzyme ATP:cob(I)alamin adenosyltransferase (ATR), which converts reduced cob(I)alamin to an active adenosylcobalamin cofactor. We recently reported presence of destabilizing pathogenic that retain some residual ATR activity. The aim present study was seek pharmacological chaperones as a tailored therapy for stabilizing protein. High-throughput ligand screening over 2000 compounds performed; six were...
Nuclear magnetic resonance (NMR) metabolomics profiling was evaluated as a new tool in sensory assessment of protein hydrolysates. Hydrolysates were produced on the basis different raw materials (cod, salmon, and chicken), enzymes (Food Pro PNL Bromelain), hydrolysis time (10 50 min). The influence material parameters attributes determined by traditional descriptive analysis 1H NMR spectroscopy. had major attribute intensity metabolite variation, followed enzyme time. However, formation...
The focus on natural foods and “clean” labeled products is increasing encourages development of new biobased ingredients. Fish solubles derived from downstream processing side stream materials in the fish filleting industries have potential as emulsifiers based their surface-active emulsion stabilizing properties. aim this study was to evaluate compare properties critical micelle concentration (CMC) direct protein extracts hydrolysates backbones, identify associations between molecular...
The structure of the chlorosome baseplate protein CsmA from Chlorobium tepidum in a 1:1 chloroform:methanol solution was determined using liquid‐state NMR spectroscopy. data reveal that 59‐residue is predominantly α‐helical with long helical domain extending residues V6 to L36, containing putative bacteriochlorophyll binding domain, and short helix C‐terminal part M41 G49. These elements are compatible model having N‐terminal stretch immersed into lipid monolayer confining protruding...
Vitellogenin (Vg) is an egg-yolk precursor protein in most oviparous species. In honeybee (Apis mellifera), the (AmVg) also affects social behavior and life-span plasticity. Despite its manifold functions, AmVg molecule remains poorly understood. The subject of our structure-oriented study polyserine tract - a little-investigated repetitive segment mostly found insects. We previously reported that tissue specifically cleaved vicinity this tract. Here, we show that, despite potential for...
A clever combination: an in situ solid-state NMR analysis of CsmA proteins the heterogeneous environment photoreceptor Chlorobaculum tepidum is reported. Using different combinations 2D and 3D spectra, 90 % resonances are assigned provide on basis chemical shift data information about structure conformation CsmA-bacteriochlorophyll a complex.
Inhibition of ClpB, the bacterial representative heat-shock protein 100 family that is associated with virulence several pathogens, could be an effective strategy to develop new antimicrobial agents. Using a high-throughput screening method, we have identified compounds bind different conformations ClpB and analyzed their effect on ATPase chaperone activities protein. Two them inhibit these functional properties as well growth Gram negative bacteria (E. coli), displaying activity under...
A series of hafnium complexes were structurally identified showing high activity (up to 500 h<sup>−1</sup>) in the selective alternated copolymerization epoxides with CO<sub>2</sub> under low pressure.
To obtain insight into the functions of proteins and their specific roles, it is important to establish efficient procedures for exploring states that encapsulate conformational space. Global Protein folding State mapping by multivariate NMR (GPS NMR) a powerful high-throughput method provides such an overview. GPS exploits unique ability simultaneously record signals from individual hydrogen atoms in complex macromolecular systems analysis describe spectral variations these few variables...