A5050220567- Protein Kinase Regulation and GTPase Signaling
- Pancreatic and Hepatic Oncology Research
- Melanoma and MAPK Pathways
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Pancreatitis Pathology and Treatment
- Radiopharmaceutical Chemistry and Applications
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Kruppel-like factors research
- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
- Long-Term Effects of COVID-19
- GDF15 and Related Biomarkers
- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Protein Degradation and Inhibitors
- Amyloidosis: Diagnosis, Treatment, Outcomes
Universitat Oberta de Catalunya
2025
Health Research Institute of the Balearic Islands
2021-2024
Hospital Universitario Son Espases
2023
Universitat de Barcelona
2011-2021
Departament de Salut
2021
Institute for Research in Biomedicine
2015
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2011-2014
Institut Català d'Oncologia
2013
Biomedical Research Institute of Lleida
2013
Institut d'Investigació Biomédica de Bellvitge
2013
Drugs that target the chief mediator of nuclear export, chromosome region maintenance 1 protein (CRM1) have potential as therapeutics for leukemia, but existing CRM1 inhibitors show variable potencies and a broad range cytotoxic effects. Here, we report structural analysis antileukemic activity new generation small-molecule CRM1. Designated selective export (SINE), these compounds were developed using molecular modeling to screen small virtual library against signal (NES) groove The 2.2-Å...
Abstract Early identification of ATTRv amyloidosis disease onset is still often delayed due to the lack validated biomarkers this disease. Light chain neurofilament (NfL) have shown promising results in early diagnosis disease, but data needed, including with alternative measuring methods. Our aim was study levels NfL measured by ELISA. Furthermore, interstitial matrix metalloproteinase type 1 (MMP-1) serum were as a potential new biomarker ATTRv. Serum and MMP-1 using ELISA assays 90...
Abstract KRAS phosphorylation has been reported recently to modulate the activity of mutant protein in vitro. In this study, we defined S181 as a specific site required license oncogenic function vivo. The phosphomutant S181A failed induce tumors mice, whereas phosphomimetic S181D exhibited an enhanced tumor formation capacity, compared with wild-type protein. Reduced growth composed cells expressing nonphosphorylatable was correlated increased apoptosis. Conversely, activation AKT and ERK,...
Abstract Better understanding on interactions between SARS‐CoV‐2 and host cells should help to identify factors that may be targetable combat infection COVID‐19 pathology. To this end, we have conducted a genome‐wide CRISPR/Cas9‐based loss‐of‐function screen in human lung cancer infected with SARS‐CoV‐2‐pseudotyped lentiviruses. Our results recapitulate many findings from previous screens used full viruses, but also unveil two novel critical factors: the lysosomal efflux transporter SPNS1...
Although COVID-19 initially caused great concern about respiratory symptoms, mounting evidence shows that also the pancreas is productively infected by SARS-CoV-2. However, severity of pancreatic SARS-CoV-2 infection and its pathophysiology still under debate. Here, we investigate consequences role host factor Placenta-associated protein (PLAC8). We analyze plasma levels enzymes inflammatory markers in a retrospective cohort study 120 patients distributed 3 severity-stratified groups....
Activating mutations in the K-Ras small GTPase are extensively found human tumors. Although these induce generation of a constitutively GTP-loaded, active form K-Ras, phosphorylation at Ser181 within C-terminal hypervariable region can modulate oncogenic function without affecting vitro affinity for its effector Raf-1. In striking contrast, phosphorylated shows increased interaction cells with Raf-1 and p110α, catalytic subunit PI 3-kinase. Because majority is located plasma membrane,...
Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes cancer-related death worldwide. It commonly diagnosed in advanced stages and therapeutic interventions are typically constrained to systemic chemotherapy, which yields only modest clinical outcomes. In this review, we examine recent developments targeted therapy tailored address distinct molecular pathway alteration required for PDAC. Our review delineates principal signaling pathways mechanisms implicated initiation...
The small G-protein Ras was the first oncogene to be identified and has a very important contribution human cancer development (20-23% prevalence). K-RasB, one of members family, is that most mutated plays prominent role in pancreatic, colon lung development. proteins are membrane bound GTPases cycle between inactive, GDP-bound active, GTP-bound, states. Most research into K-RasB activity regulation focused on analysis how GTP-exchange factors (GEFs) GTPase activating (GAPs) regulated by...
Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes cancer-related death worldwide. It commonly diagnosed in advanced stages and therapeutic interventions are typically constrained to systemic chemotherapy, which yields only modest clinical outcomes. In this review, we examine recent developments targeted therapy tailored address distinct molecular pathway alteration required for PDAC. Our review delineates principal signaling pathways mechanisms implicated initiation...
Coronavirus disease 19 (COVID-19) is a persistent global pandemic with very heterogeneous presentation ranging from mild to dismal prognosis. Early detection of sensitivity and severity COVID-19 essential for the development new treatments. In present study, we measured levels circulating growth differentiation factor 15 (GDF15) angiotensin-converting enzyme 2 (ACE2) in plasma severity-stratified patients uninfected control characterized vitro effects cohort frequency ACE2 SNPs. Our results...
Mitosis entails mechanistic changes required for maintaining the genomic integrity in all dividing cells. The process is intricate and temporally spatially regulated by ordered series of activation de-activation protein kinases. mitotic kinases ensure stepwise progression entry into mitosis after G2 phase cell cycle, followed prophase, pro-metaphase, metaphase, anaphase, telophase, subsequently cytokinesis birth two daughter cells with equal segregation distribution genome. major include...
Abstract Background Although COVID-19 initially caused great concern about respiratory symptoms, mounting evidence shows that also the pancreas is productively infected by SARS-CoV-2. However, severity of pancreatic SARS-CoV-2 infection and its pathophysiology are still under debate. Here we investigated consequences role host factor Placenta-associated protein (PLAC8) Methods We analyzed plasma levels enzymes inflammatory markers in a retrospective cohort study 120 patients distributed 3...
The biology of aging is focused on the identification novel pathways that regulate underlying processes to develop interventions aimed at delaying onset and progression chronic diseases extend lifespan. However, research field has been conducted mainly in animal models, yeast, Caenorhabditis elegans cell culture. Thus, it unclear what extent this knowledge transferable humans since they might not reflect complexity people. Organoid culture an vitro 3D cell-culture technology reproduces...
<div>Abstract<p>KRAS phosphorylation has been reported recently to modulate the activity of mutant KRAS protein <i>in vitro</i>. In this study, we defined S181 as a specific site required license oncogenic function vivo</i>. The phosphomutant S181A failed induce tumors in mice, whereas phosphomimetic S181D exhibited an enhanced tumor formation capacity, compared with wild-type protein. Reduced growth composed cells expressing nonphosphorylatable was correlated...
<p>PDF file - 646K, Fig. S3. Histological pattern of mice grafts derived from NIH3T3 expressing phosphomutants.</p>
<p>PDF file - 25K, Fig. S5. GTP loading of endogenous Ras isoforms in the DLD1 KRAS wt/- cells expressing oncogenic phosphomutants.</p>
<p>PDF file - 25K, Fig. S2. Quantitation of apoptosis by cleaved caspase-3 measurement.</p>
<p>PDF file - 31K, Fig. S4. Treatment with PKC inhibitor Edelfosine inhibits tumor growth of HAKRASG12V in a Ser181 KRAS phosphorylation-dependent fashion.</p>