A5020225471- Mast cells and histamine
- Liver physiology and pathology
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Liver Diseases and Immunity
- Pediatric Hepatobiliary Diseases and Treatments
- Organ Transplantation Techniques and Outcomes
- Clinical Nutrition and Gastroenterology
- Amino Acid Enzymes and Metabolism
- Oral and gingival health research
- Fibroblast Growth Factor Research
- Polyamine Metabolism and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cannabis and Cannabinoid Research
- Diet, Metabolism, and Disease
- Neuroendocrine Tumor Research Advances
- Alcohol Consumption and Health Effects
- Digestive system and related health
- Peroxisome Proliferator-Activated Receptors
- Diet and metabolism studies
- Neuropeptides and Animal Physiology
- Peptidase Inhibition and Analysis
- Pharmacological Receptor Mechanisms and Effects
- Vitamin D Research Studies
- Garlic and Onion Studies
Texas A&M University
2017-2021
Bryan College
2020-2021
Institute of Neurobiology
2020
Temple College
2017-2019
Texas A&M Health Science Center
2013-2019
Central Texas Veterans Health Care System
2014-2018
Baylor Scott & White Health
2013-2017
Virginia Commonwealth University
2017
Texas A&M University System
2017
Canon (Japan)
2016
Hepatic fibrosis is marked by activation of hepatic stellate cells (HSCs). Cholestatic injury precedes liver fibrosis, and cholangiocytes interact with HSCs promoting fibrosis. Mast (MCs) infiltrate following release histamine, increasing biliary proliferation. We evaluated if inhibition MC-derived histamine decreases proliferation Wild-type multidrug resistance 2 knockout mice (9-11 weeks) were treated cromolyn sodium for 1 week to block histamine. Biliary mass immunohistochemistry...
Primary sclerosing cholangitis (PSC) patients are at risk of developing cholangiocarcinoma (CCA). We have shown that (1) histamine increases biliary hyperplasia through H1/H2 receptors (HRs) and (2) levels increase mast cells (MCs) infiltrate during PSC CCA. examined the effects chronic treatment with H1/H2HR antagonists on Wild-type multidrug-resistant knockout (Mdr2-/- ) mice were treated by osmotic minipumps saline, mepyramine, or ranitidine (10 mg/kg body weight/day) a combination...
Background and Aims Cholestasis is characterized by increased total bile acid (TBA) levels, which are regulated farnesoid X receptor (FXR)/FGF15. Patients with primary sclerosing cholangitis (PSC) typically present inflammatory bowel disease (IBD). Mast cells (MCs) (i) express FXR (ii) infiltrate the liver during cholestasis promoting fibrosis. In bile‐duct‐ligated (BDL) MC‐deficient mice (B6.Cg‐ KitW‐sh /HNihrJaeBsmJ [ ]), ductular reaction (DR) fibrosis decrease compared BDL wild type, MC...
Background and Aims Nonalcoholic fatty liver disease (NAFLD) is simple steatosis but can develop into nonalcoholic steatohepatitis (NASH), characterized by inflammation, fibrosis, microvesicular steatosis. Mast cells (MCs) infiltrate the during cholestasis promote ductular reaction (DR), biliary senescence, fibrosis. We aimed to determine effects of MC depletion NAFLD/NASH. Approach Results Wild‐type (WT) KitW‐sh (MC‐deficient) mice were fed a control diet (CD) or Western (WD) for 16 weeks;...
Activated mast cells (MCs) release histamine (HA) and MCs infiltrate the liver following bile duct ligation (BDL), increasing intrahepatic mass (IBDM) fibrosis. We evaluated effects of BDL in MC‐deficient ( KitW‐sh ) mice. Wild‐type (WT) mice were subjected to sham or for up 7 days injected with cultured 1× phosphate‐buffered saline (PBS) before collecting serum, liver, cholangiocytes. Liver damage was assessed by hematoxylin eosin alanine aminotransferase levels. IBDM detected...
Cholestasis is a condition that leads to chronic hepatobiliary inflammation, fibrosis, and eventually cirrhosis. Many microRNAs (miRs) are known have role in fibrosis progression; however, the of miR-21 during cholestasis remains unknown. Therefore, aim this study was elucidate cholestasis-induced biliary hyperplasia hepatic fibrosis. Wild-type (WT) miR-21-/- mice underwent Sham or bile duct ligation (BDL) for 1 week, before evaluating liver histology, proliferation, stellate cell (HSC)...
Background and Aims Following liver injury, mast cells (MCs) migrate into the are activated in patients with cholestasis. Inhibition of MC mediators decreases ductular reaction (DR) fibrosis. Transforming growth factor beta 1 (TGF‐β1) contributes to fibrosis promotes disease. Our aim was demonstrate that reintroduction MCs induces cholestatic injury through TGF‐β1. Approach Results Wild‐type, KitW‐sh (MC‐deficient), multidrug resistance transporter 2/ABC B family member 2 knockout mice...
Substance P (SP) promotes cholangiocyte growth during cholestasis by activating its receptor, NK1R. SP is a proteolytic product of tachykinin (Tac1) and deactivated membrane metalloendopeptidase (MME). This study aimed to evaluate the functional role in regulation cholangiocarcinoma (CCA) growth. NK1R, Tac1, MME expression secretion were assessed human CCA cells nonmalignant cholangiocytes. The proliferative effects (in absence/presence NK1R inhibitor, L-733,060) L-733,060 evaluated. In...
Histidine is converted to histamine by histidine decarboxylase (HDC). We have shown that cholangiocytes 1) express HDC, 2) secrete histamine, and 3) proliferate after treatment via ERK1/2 signaling. In bile duct-ligated (BDL) rodents, there enhanced biliary hyperplasia, HDC expression, secretion. This studied aimed demonstrate knockdown of inhibits proliferation downregulation PKA/ERK1/2 HDC(-/-) mice matching wild-type (WT) were subjected sham or BDL. After 1 wk, serum, liver blocks,...
This study aimed to establish mechanistic links between the prolonged intake of desloratadine, a common H1 receptor blocker (i.e., antihistamine), and development obesity metabolic syndrome. Male Sprague-Dawley rats were treated for 16 wk with desloratadine. We analyzed dynamics body weight gain, tissue fat accumulation/density, contractility isolated mesenteric lymphatic vessels, levels blood lipids, glucose, insulin, together parameters liver function. Prolonged desloratadine induced an...
The functions of the liver are very diverse. From detoxifying blood to storing glucose in form glycogen and producing bile facilitate fat digestion, is a active important organ. comprised many varied cell types whose equally Cholangiocytes line biliary tree aid transporting adjusting composition as it travels gallbladder. Hepatic stellate cells portal fibroblasts located different areas within architecture, but both contribute development fibrosis upon activation after injury. Vascular...