A5061207415- Lysosomal Storage Disorders Research
- RNA and protein synthesis mechanisms
- Trypanosoma species research and implications
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Atherosclerosis and Cardiovascular Diseases
- RNA Research and Splicing
- Platelet Disorders and Treatments
- RNA modifications and cancer
- Genetics, Bioinformatics, and Biomedical Research
- Hemophilia Treatment and Research
- Blood Coagulation and Thrombosis Mechanisms
- Machine Learning in Bioinformatics
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Autoimmune and Inflammatory Disorders Research
- Genetic Syndromes and Imprinting
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Complement system in diseases
- Cellular transport and secretion
- Nitric Oxide and Endothelin Effects
- RNA Interference and Gene Delivery
- Biomarkers in Disease Mechanisms
- Electron Spin Resonance Studies
Center for Biologics Evaluation and Research
2022-2025
United States Food and Drug Administration
2022-2025
University of Michigan
2012-2018
Abstract Monoclonal antibodies (mAbs) are commonly used biologic therapeutics with a wide variety of clinical applications. During the development process, manufacturers consider different production parameters to improve protein yield and achieve appropriate quality product. Synonymous gene recoding is one such attributes that often considered implemented enhance expression. However, it has be caution, as may lead misfolding ER stress, which complicates efforts manufacture desired mAb. To...
Fabry disease results from loss of activity the lysosomal enzyme α-galactosidase A (GLA), leading to accumulation globoseries glycosphingolipids in vascular endothelial cells. Thrombosis and stroke are life-threatening complications disease; however, mechanism vasculopathy remains unclear. We explored relationship between GLA deficiency cell von Willebrand factor (VWF) secretion vivo vitro models disease. Plasma VWF was significantly higher at two months increased with age Gla-null compared...
Reactive oxygen species are a key mediator of myocardial reperfusion injury. Endogenous cellular defenses against reactive often become overwhelmed after ischemia and reperfusion. Therefore, exogenous supplementation various antioxidant compounds has been hypothesized to protect Reduced glutathione (GSH) is an important endogenous that affords protection oxidative damage. Oral administration GSH limited due poor gastrointestinal absorption. A liposomal preparation (lipGSH) capable oral was...
Fabry disease is an X-linked disorder caused by a defect in the gene encoding lysosomal hydrolase, alpha-galactosidase A (GLA). The loss GLA function causes age-dependent accumulation of globotriaosylceramide endothelial cells, which results decreased nitric oxide bioavailability and premature mortality affected patients. Based on this, we hypothesized that deficiency promotes inflammation. We observed significant increase plasma von Willebrand Factor (vWF) mice compared to age-matched wild...