A5056557106- Proteins in Food Systems
- Food Allergy and Anaphylaxis Research
- Protein Hydrolysis and Bioactive Peptides
- Microplastics and Plastic Pollution
- Allergic Rhinitis and Sensitization
- Dermatology and Skin Diseases
- Advanced Glycation End Products research
- Protein Interaction Studies and Fluorescence Analysis
- Tea Polyphenols and Effects
- Hemoglobin structure and function
- Algal biology and biofuel production
- Recycling and Waste Management Techniques
- Effects and risks of endocrine disrupting chemicals
- Phytochemicals and Antioxidant Activities
- Microencapsulation and Drying Processes
- Nitric Oxide and Endothelin Effects
- Contact Dermatitis and Allergies
- biodegradable polymer synthesis and properties
- Consumer Attitudes and Food Labeling
- Erythrocyte Function and Pathophysiology
- Biochemical effects in animals
- Neuroendocrine regulation and behavior
- Neonatal Health and Biochemistry
- Dye analysis and toxicity
- Occupational exposure and asthma
University of Belgrade
2016-2025
University of Belgrade – Faculty of Chemistry
2014-2024
Faculty (United Kingdom)
2015-2016
University of Zagreb
2012
Institute of Chemistry, Technology and Metallurgy
2009
Institute for Technology of Nuclear and other Mineral Raw Materials
2004-2009
Centre of Research Excellence in Nutrition and Metabolism
2007
Abstract Digestion is the key step for delivering nutrients and bioactive substances to body. The way different food components interact with each other digestive enzymes can modify digestion process affect human health. Understanding how during essential rational design of functional products. Plant polyphenols have gained much attention roles they play in However, their strong beneficial effects on health also been associated a negative impact process. Due generally low absorption phenolic...
Scope The aim of this work was to investigate the effects high‐intensity ultrasound (sonication), on structure and allergenicity major cow's milk allergen, beta‐lactoglobulin ( BLG ). Methods results Structural changes upon sonication were monitored by circular dichroism spectroscopy, tryptophan emission fluorescence, hydrophobic dye retinol binding, as well digestibility phenol‐oxidase cross‐linking capacity. Allergenicity in individual patients’ sera, basophil activation test, skin prick...
Abstract Conglutins represent the major peanut allergens and are renowned for their resistance to gastro-intestinal digestion. Our aim was characterize digestion-resistant peptides (DRPs) of conglutins by biochemical biophysical methods followed a molecular dynamics simulation in order better understand basis food protein allergenicity. We have mapped proteolysis sites at N- C-termini limited internal segment, while other potential remained unaffected. Molecular showed that only occurred...
Contaminating microplastics can interact with food proteins in the matrix and during digestion. This study investigated adsorption of chicken egg protein ovalbumin to polystyrene (PS, 110 260 μm) polyethylene terephthalate (PET, 140 MPs acidic neutral conditions alterations structure. Ovalbumin affinity depended on size (smaller > larger), type (PS PET) pH (pH 3 7). In bulk solution, does not change secondary structure significantly, but induces loosening (at 3) tightening 7) tertiary Formed...
Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim this study was to examine the effects enzymatic crosslinking on IgE binding, allergenicity and digestion stability beta-casein (CN). beta-CN crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid laccase/caffeic acid. binding compared vitro CAP inhibition assay, ELISA as well ex vivo basophil activation assay. Crosslinked CNs were digested simulated gastric fluid for 15 60 min obtained...
Scope During food processing, the Maillard reaction (МR) may occur, resulting in formation of glycated proteins. Glycated proteins are particular importance allergies because glycation influence interactions with immune system. This study compared native and extensively milk allergen β‐lactoglobulin (BLG), their cells crucially involved allergy. Methods results BLG was MR characterized. Native were tested experiments epithelial transport, uptake degradation by DCs, T‐cell cytokine responses,...
Summary Background Most food allergens sensitizing via the gastrointestinal tract are stable proteins that resistant to pepsin digestion, in particular major peanut allergens, Ara h 2 and 6. Survival of their large fragments is essential for capacity. However, immunoreactive proteins/peptides which immune system exposed during digestion unknown. Particularly, IgE reactivity short digestion‐resistant peptides (SDRPs; <10 kDa) released by gastric under standardized physiologically relevant...
Phycocyanobilin (PCB) binds with high affinity (2.2 x 106 M-1 at 25°C) to human serum albumin (HSA) sites located in IB and IIA subdomains. The aim of this study was examine effects PCB binding on protein conformation stability. Using 300 ns molecular dynamics (MD) simulations, UV-VIS spectrophotometry, CD, FT-IR, spectrofluorimetry, thermal denaturation susceptibility trypsin digestion, we studied the stability rigidity HSA, as well conformational changes itself upon protein. MD simulation...
Human ingestion of microplastics (MPs) is common and inevitable due to the widespread contamination food items, but implications on gastric digestion proteins are still unknown. In this study, interactions between pepsin polystyrene (PS) MPs were evaluated by investigating enzyme activity conformation in a simulated human environment presence or absence PS MPs. The impact was also assessed monitoring kinetics protein hydrolysis through static vitro cow's milk contaminated with PS. binding...