A5085021707- Food Allergy and Anaphylaxis Research
- Adipose Tissue and Metabolism
- Nutritional Studies and Diet
- Blood Coagulation and Thrombosis Mechanisms
- Allergic Rhinitis and Sensitization
- Protease and Inhibitor Mechanisms
- Fatty Acid Research and Health
- Contact Dermatitis and Allergies
- Proteins in Food Systems
- Essential Oils and Antimicrobial Activity
- Protein Hydrolysis and Bioactive Peptides
- Signaling Pathways in Disease
- Phytochemicals and Antioxidant Activities
- Occupational exposure and asthma
- Eosinophilic Esophagitis
- SARS-CoV-2 detection and testing
- Advanced Nanomaterials in Catalysis
- Reproductive tract infections research
- Dermatology and Skin Diseases
- Urinary Tract Infections Management
- Meat and Animal Product Quality
- Protein Kinase Regulation and GTPase Signaling
- Olfactory and Sensory Function Studies
- SARS-CoV-2 and COVID-19 Research
- Adipokines, Inflammation, and Metabolic Diseases
University of Belgrade
2016-2025
University of Belgrade – Faculty of Chemistry
2014-2024
Institut za filozofiju
2020
Hydrolates, also referred to as hydrosols, floral or distillate waters, well aromatic are produced in the same isolation process with essential oils by steam distillation. A small amount of oil constituents is dissolved hydrolates providing specific organoleptic properties and flavor, biological activity which makes them useful raw material many industries. Their popularity still on rise, especially aromatherapy. The objective this review analyze chemical compositions their corresponding...
This study evaluates the efficiency of 20 Natural Deep Eutectic Solvents (NADES) formulations for extracting curcuminoids and other bioactive compounds from turmeric emphasize their ability to preserve enhance antioxidant, antimicrobial, antidiabetic, skin depigmentation effects. The NADES formulations, prepared using choline chloride (ChCl) combined with sugars, carboxylic acids, glycerol, amino urea, polyols, betaine, were assessed extraction based on total phenolic content curcumin...
The selective hydrolysis of proteins by non-enzymatic catalysis is difficult to achieve, yet it crucial for applications in biotechnology and proteomics. Herein, we report that discrete hafnium metal-oxo cluster [Hf18 O10 (OH)26 (SO4 )13 ⋅(H2 O)33 ] (Hf18 ), which centred the same hexamer motif found many MOFs, acts as a heterogeneous catalyst efficient horse heart myoglobin (HHM) low buffer concentrations. Among 154 amino acids present sequence HHM, strictly cleavage at only 6 solvent...
Summary Background Most food allergens sensitizing via the gastrointestinal tract are stable proteins that resistant to pepsin digestion, in particular major peanut allergens, Ara h 2 and 6. Survival of their large fragments is essential for capacity. However, immunoreactive proteins/peptides which immune system exposed during digestion unknown. Particularly, IgE reactivity short digestion‐resistant peptides (SDRPs; <10 kDa) released by gastric under standardized physiologically relevant...
The<italic>in vitro</italic>anti-cervical cancer potency of tested polyphenol extracts is exhibited in the following order: green tea > coffee cocoa, with only showing both pro-oxidative and anti-proliferative action.
As byproducts of essential oil distillation, hydrolates are used in natural cosmetics/biomedicine due to their beneficial skin effects. However, data on safety with relevant biological targets, such as human cells, scarce. Therefore, we have tested nine from the Lamiaceae family fibroblasts that responsible for extracellular collagenous matrix builds. Thyme, oregano, and winter savoury showed several times higher total phenolics, which correlated strongly radical scavenging antioxidative...
The binding of ubiquitous serum ligands (free fatty acids) to human albumin (HSA) or its glycation can affect thiol group reactivity, thus influencing antioxidant activity. effects stearic acid (SA) and glucose on HSA structural changes content reactivity were monitored by fluoroscopy the Ellman method during a 14-day incubation in molar ratios that mimic pathophysiological conditions. Upon with 5 mM glucose, was same as without it, three different HSA:SA (HSA:SA-1:1-2-4). protective effect...
Short ragweed (Ambrosia artemisiifolia) allergies affect more than 36 million people annually. Ragweed pollen grains release subpollen particles (SPP) of respirable size upon hydration or a change in air electrical conditions. The aim this study was to characterize the proteomes and allergomes short SPP total protein extract (TOT), compare their effects with those standard aqueous (APE) using sera from pollen-sensitized patients.Quantitative 2D gel-based shotgun proteomics, 1D...
An association between pollution (e.g., from traffic emissions) and the increased prevalence of respiratory allergies has been observed. Field-realistic exposure studies provide most relevant assessment effects intensity diversity urban industrial contamination on pollen structure allergenicity. The significance in-depth post-translational modification (PTM) proteomes, when compared with other aspects altered allergenicity, not yet determined; hence, little progress made within this field....
Abstract Background Although still considered a safer alternative to classical cigarettes, growing body of work points harmful effects electronic cigarettes (e-cigarettes) affecting range cellular processes. The biological effect e-cigarettes needs be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration e-cigarette liquids, and without nicotine. Mutagenicity was evaluated by HPRT assay,...
Resistance to digestion by digestive proteases represents a critical property of many food allergens. Recently, harmonized INFOGEST protocol was proposed for solid digestion. The proposes conditions suitable all kinds and liquid foods. However, peanuts, as lipid-rich food, represent challenge downstream analyses the digestome. This is particularly reflected in methodological difficulties analyzing proteins peptides presence lipids. Therefore, removal lipids seems be prerequisite analysis...
Post-translational modifications (PTMs) are covalent changes occurring on amino acid side chains of proteins and yet neglected structural functional aspects protein architecture. The objective was to detect differences in PTM profiles that take place after roasting using open search. We conducted a bottom-up proteomic study investigate the impact peanut readily soluble allergens their profiles. Proteomic profiling certain confirmed by Western blotting with series PTM-specific antibodies. In...
Abstract The selective hydrolysis of proteins by non‐enzymatic catalysis is difficult to achieve, yet it crucial for applications in biotechnology and proteomics. Herein, we report that discrete hafnium metal‐oxo cluster [Hf 18 O 10 (OH) 26 (SO 4 ) 13 ⋅(H 2 O) 33 ] ( Hf ), which centred the same hexamer motif found many MOFs, acts as a heterogeneous catalyst efficient horse heart myoglobin (HHM) low buffer concentrations. Among 154 amino acids present sequence HHM, strictly cleavage at only...
Abstract Insulin (INS) via INS receptor acts as a mitogen in vascular smooth muscle cells (VSMCs) through stimulation of multiple signaling mechanisms, including p42/44 mitogen‐activated protein kinase (ERK1/2) and phosphatidyl inositol‐3 (PI3K). In addition, cytosolic phospholipase 2 (cPLA ) is linked to VSMCs proliferation. However, the upstream mechanisms responsible for activation cPLA are not well defined. Therefore, this investigation used primary cultured rat examine role PI3K ERK1/2...