A5067551716- HIV Research and Treatment
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Antimicrobial Resistance in Staphylococcus
- Immune responses and vaccinations
- Influenza Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
- Radiopharmaceutical Chemistry and Applications
- T-cell and Retrovirus Studies
- HIV-related health complications and treatments
- HIV/AIDS Research and Interventions
- Respiratory viral infections research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- interferon and immune responses
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Disease Management and Epidemiology
- Chronic Lymphocytic Leukemia Research
- Viral gastroenteritis research and epidemiology
GlaxoSmithKline (United States)
2021-2023
Ragon Institute of MGH, MIT and Harvard
2017-2021
Novartis (Italy)
2014-2016
Sapienza University of Rome
2014-2016
Novartis (Switzerland)
2015
Toscana Life Sciences
2015
Both active and passive immunization strategies against Staphylococcus aureus have thus far failed to show efficacy in humans. With the attempt develop an effective S. vaccine, we selected five conserved antigens known different roles pathogenesis. They include secreted factors α-hemolysin (Hla), ess extracellular A (EsxA), B (EsxB) two surface proteins ferric hydroxamate uptake D2 staphylococcal antigen 1A. The combined vaccine formulated with aluminum hydroxide induced antibodies...
RNA vaccines have demonstrated efficacy against SARS-CoV-2 in humans, and the technology is being leveraged for rapid emergency response. In this report, we assessed immunogenicity and, first time, toxicity, biodistribution, protective preclinical models of a two-dose self-amplifying messenger (SAM) vaccine, encoding prefusion-stabilized spike antigen Wuhan-Hu-1 strain delivered by lipid nanoparticles (LNPs). mice, one immunization with SAM vaccine elicited robust spike-specific antibody...
HIV-specific broadly neutralizing antibodies (bNAbs) confer protection after passive immunization, but the immunological mechanisms that drive their development are poorly understood. Structural features of bNAbs indicate they originate from extensive germinal center (GC) selection, which relies on persistent GC activity. However, why a fraction infected individuals able to successfully more effective affinity maturation is unclear. Delivery antigens in form antibody-immune complexes (ICs),...
Abstract Induction of persistent protective immune responses is a key attribute successful vaccine formulation. MF59 adjuvant, an oil-in-water emulsion used in human vaccines, known to induce high-affinity functional Ab titers and memory B cells, but how it really shapes the Ag-specific cell compartment poorly documented. In this study, we characterized Ab- wild-type mice immunized with HlaH35L, Staphylococcus aureus Ag measurable responses, formulated or aluminum salts, focusing on germinal...
A rapidly acting, single dose vaccine against Staphylococcus aureus would be highly beneficial for patients scheduled major surgeries or in intensive care units. Here we show that one immunization with a multicomponent S. candidate vaccine, 4C-Staph, formulated novel TLR7-dependent adjuvant, T7-alum, readily protected mice from death and bacterial dissemination, both kidney abscess peritonitis models, outperforming alum-formulated vaccine. This increased efficacy was paralleled by higher...
Understanding the mechanism(s) by which broadly neutralizing antibodies (bNAbs) emerge naturally following infection is crucial for development of a protective vaccine against human immunodeficiency virus (HIV). Although previous studies have implicated high viremia and associated immune activation as potential drivers bNAbs, here we sought to unlink effect these 2 parameters evaluating key inflammatory predictors bNAb in HIV-infected individuals who spontaneously control HIV absence...
Changes in antibody glycosylation are linked to inflammation across several diseases. Alterations bulk galactosylation can predict rheumatic flares, act as a sensor for immune activation, gastric cancer relapse, track with biological age, shift vaccination, change HIV reservoir size on therapy, and decrease HCV infections. However, whether changes Fc biology also rebound time remains unclear. The identification of biomarker that could forecast viral significantly accelerate the downselection...
Staphylococcus aureus (S. aureus) is an important opportunistic pathogen that may cause invasive life-threatening infections like sepsis and pneumonia. Due to increasing antibiotic-resistance, the development of effective vaccine against S. needed. Although a correlate protection staphylococcal diseases not yet established, several findings suggest both antibodies CD4 T cells might contribute optimal immunity. In this study, we show adjuvanting multivalent (4C-Staph) with MF59, oil-in-water...
A minor subset of individuals infected with HIV-1 develop antibody neutralization breadth during the natural course infection, often linked to chronic, high-level viremia. Despite significant efforts, vaccination strategies have been unable induce similar and mechanisms underlying neutralizing induction remain largely elusive. Broadly responses can also be found in who control HIV low even undetectable plasma levels absence antiretroviral therapy, suggesting that high antigen exposure is not...
RNA vaccines are instrumental to a timely response disease outbreaks and pandemics. However, drug development constantly copes with high attrition rates, leading efficacy safety-related failures. Mathematical models cost time-effective tools in guiding optimization strategies. Here, we extend multicompartmental model of immunogenicity for therapeutic proteins1 provide representation the early events after injection an mRNA vaccine suggest, through sensitivity analysis, properties optimize....
Although influenza vaccination remains the most effective way to protect population from infection, current Flu vaccines induce suboptimal immune responses in particular segments of and need be reformulated almost every year due genetic instability virus. The development next generation represents a fascinating challenge for scientists clinicians, who aim design new vaccine formulations that are able cross-protective immunity against multiple strains promote long-lasting responses. With this...
Abstract A minor subset of individuals infected with HIV-1 develop antibody neutralization breadth during the natural course infection, often linked to chronic, high level viremia. Despite significant efforts, vaccination strategies have been unable induce similar and mechanisms underlying neutralizing induction remain largely elusive. Broadly responses can also be found in who control HIV low even undetectable plasma levels absence antiretroviral therapy, suggesting that antigen exposure is...
Abstract Primary exposure to an antigen generates, along with antibody-secreting cells (ASCs), a heterogeneous B-cell memory compartment that changes over time and may respond in different ways when re-encountering the cognate antigen. The goal of this study is characterize responses after priming effect boosting intervals on differentiation function ASCs B cells. We used immunization strategy based Hla protein from S. aureus, adjuvanted Alum, as it was previously reported induce protective...
Event Abstract Back to Vaccine-mediated B cells responses S. aureus hemolysin, and effect of different boosting intervals on functional humoral Giuseppe Lofano1*, Maria Teresa Fiorillo1 Sylvie Bertholet2 1 University Rome "Sapienza", Italy 2 Novartis V&D, Although antibody titers are usually considered the best readout for vaccine efficacy, importance memory B-cell in conferring protection has remained controversial. Primary exposure an antigen generates, along with antibody-secreting...