A5013239716- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- Immunodeficiency and Autoimmune Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Malaria Research and Control
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Parvovirus B19 Infection Studies
- Insect Resistance and Genetics
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
GlaxoSmithKline (United States)
2017-2022
Vertex Pharmaceuticals (United States)
2012
RNA vaccines have demonstrated efficacy against SARS-CoV-2 in humans, and the technology is being leveraged for rapid emergency response. In this report, we assessed immunogenicity and, first time, toxicity, biodistribution, protective preclinical models of a two-dose self-amplifying messenger (SAM) vaccine, encoding prefusion-stabilized spike antigen Wuhan-Hu-1 strain delivered by lipid nanoparticles (LNPs). mice, one immunization with SAM vaccine elicited robust spike-specific antibody...
Human cytomegalovirus (HCMV) is the leading viral cause of birth defects and organ transplant rejection. The HCMV gH/gL/UL128/UL130/UL131A complex (Pentamer) main target humoral responses thus a key vaccine candidate. We report two structures Pentamer bound to human neutralizing antibodies, 8I21 9I6, at 3.0 5.9 Å resolution, respectively. gH/gL architecture similar that Epstein-Barr virus (EBV) except for amino-terminal extensions on both subunits. extension gL forms subdomain composed...
A Zika self-amplifying mRNA vaccine delivered by a flexible bedside mixing formulation induces protective immunity in NHPs.
Abstract Predicting the efficacy and toxicity of intramuscular mRNA vaccines remains challenging. Here, we describe an ex vivo human cell-based model that replicates immune responses to lipid nanoparticle (LNP)-based require injection. Vaccines are administered into a biomimetic muscle module containing skeletal myoblasts antigen-presenting cells (APCs) mimic vaccination, followed by transfer APCs soluble factors microfluidic lymphoid follicle chip (LF Chip) lymphatic drainage....