Diabetes increases gingival collagenase activity, an effect that may be mediated by endogenous tissue changes and exacerbated overgrowth of Gram‐negative organisms in the crevice (see Ramamurthy & Golub 1983, McNamara et al. 1982). In attempt to reverse this collagenolytic abnormality, we administered appropriate antibiotic, minocycline (a semisynthetic tetracycline), diabetic rats humans. Adult male conventional or germfree were made with streptozotocin, half these animals (20 mg per...

Tetracyclines (including the semi-synthetic analogues, minocycline and doxycycline) are considered useful adjuncts in periodontal therapy because they suppress Gram-negative periodontopathogens. Recently, these antibiotics were found to inhibit mammalian collagenase activity, a property which may also be of therapeutic value. It has been suggested that anti-collagenase properties tetracyclines independent their antibiotic efficacy. To advance this hypothesis further, we chemically converted...

Abstract We previously reported that low‐dose doxycycline (DOXY) therapy reduces host‐derived collagenase activity in gingival tissue of adult periodontitis (AP) patients. However, it was not clear whether this vivo effect direct or indirect. In the present study, inflamed human tissue, obtained from AP patients during periodontal surgery, extracted and extracts partially purified by (NH4) 2 SO 4 precipitation. The were then analyzed for using SDS‐PAGE/fluorography/laser densitometry,...

Tetracyclines are now recognized to have non‐antimicrobial properties with therapeutic potential ‐ for example, these agents can inhibit pathologic collagenolysis by blocking mammalian collagenases and other matrix‐degrading metal‐loproteinases. In the current study, adult human subjects moderate chronic periodontitis were administered specially formulated capsules of doxycycline, containing lower‐than‐usual amounts this semisynthetic tetracycline, on a daily basis 2 weeks prior...

Recently, a new mechanism was proposed to explain the therapeutic efficacy of tetracyclines in periodontal disease— that specifically these antibiotics directly inhibit activity collagenase (and possibly other collagenolytic enzymes) produced by host tissues resulting reduced rate collagen breakdown (Golub et al . 1983). In current studies, effect (minocycline, doxycycline, tetracycline) on enzyme examined human pockets and several animal systems. clinical gingival crevicular fluid (GCF)...

Background— Vulnerable plaque demonstrates intense inflammation in which macrophages secrete matrix metalloproteinases (MMPs) that degrade the fibrous cap, ultimately leading to rupture, situ thrombosis, and an associated clinical event. Thus, inhibition of MMP activity or more general suppression vascular are attractive targets for interventions intended reduce rupture. We hypothesized subantimicrobial doses doxycycline (SDD) (20 mg twice daily) would benefit patients with coronary artery...

Collagenase activity in human gingival fluid was measured using a radioactive collagen fibril assay. The positively correlated with the severity of disease. collagenase seemed to be controlled by α 2 -macroglobulin, based on its activation NaSCN, and present solely extracellular fraction. Examination breakdown products acrylamide gel electrophoresis indicated that tissue rather than bacterial origin.

Abstract Objectives: The therapeutic effects of doxycycline and other tetracyclines in the treatment periodontitis involve, at least part, mechanisms that are unrelated to their antimicrobial activity. Previous clinical studies have shown administered orally, doses below those needed for efficacy, human subjects with adult resulted significantly reduced collagenase activity gingival crevicular fluid (GCF) extracts inflamed tissues. purpose present study was identify clinically effective...

Abstract Antibiotic forms of tetracycline exhibit antitumor activity in some tumor models. However, their low vivo efficacy and associated morbidity limit long‐term application cancer therapy. This report appraises the doxycycline (DC) non‐antimicrobial, chemically modified tetracyclines (CMTs) against prostate cancer. Both DC several CMTs inhibited cell proliferation vitro . Some were significantly more potent than DC. One CMTs, 6‐deoxy, 6‐demethyl, 4‐de‐dimethylamino (CMT‐3, COL‐3), was...

T etracyclines (TC s ) have wide therapeutic usage as antimicrobial agents; these drugs (e.g., minocycline, doxycycline) remain useful adjuncts in periodontal therapy. However, TCs also non‐antimicrobial properties which appear to modulate host response. In that regard, and their chemically‐modified analogs (CMTs) been shown inhibit the activity of matrix metalloproteinase (MMP), collagenase. The this enzyme appears crucial destruction major structural protein connective tissues, collagen....

Background: Administration of subantimicrobial dose doxycycline (SDD) to chronic periodontitis (CP) patients has repeatedly been found reduce mammalian collagenase and other matrix metalloproteinase (MMP) activity in gingival tissues crevicular fluid, association with clinical efficacy, without the emergence antibiotic‐resistant bacteria either orally or extra‐orally. More recently, SDD adjunctive repeated mechanical debridement resulted dramatic improvement (>50% smokers) generalized...

• Recent evidence has demonstrated a marked anticollagenolytic effect for the tetracycline antibiotics. We have examined efficacy of systemic hydrochloride in rabbit model corneal ulceration. A standard alkali burn (1N sodium hydroxide 40 s) was delivered to 62 eyes (31 rabbits). Animals were then divided into three groups: group 1 (controls) (ten rabbits [20 eyes]) received no treatment; 2 10 mg/kg/d hydrochloride, given intramuscularly; and 3 (11 [22 50 mg/kg/d. Ulceration occurred 85%...

We recently demonstrated that a 2-year subantimicrobial-dose doxycycline (SDD) regimen (double-masked, placebo-controlled clinical trial) in postmenopausal (PM) women exhibiting mild systemic bone loss (osteopenia) and local (periodontitis) reduced the progression of periodontal attachment (intent-to-treat analysis) severity gingival inflammation alveolar (subgroups) without producing antibiotic side effects. now describe SDD effects on biomarkers collagen degradation resorption crevicular...