A5029170473- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Cardiac pacing and defibrillation studies
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Computational Drug Discovery Methods
- Chemistry and Stereochemistry Studies
- HIV Research and Treatment
- Analytical Chemistry and Sensors
- Mosquito-borne diseases and control
- Cardiac Valve Diseases and Treatments
- COVID-19 epidemiological studies
- Gas Sensing Nanomaterials and Sensors
- Cardiac Imaging and Diagnostics
- Immune Cell Function and Interaction
- Advanced MRI Techniques and Applications
- Advanced Chemical Sensor Technologies
- COVID-19 Clinical Research Studies
- Cancer-related molecular mechanisms research
- Neurological disorders and treatments
- Data-Driven Disease Surveillance
Zhongshan Hospital
2022-2025
Fudan University
2017-2025
Beijing Anzhen Hospital
2023-2025
Capital Medical University
2023-2025
Tianjin Medical University
2025
Queen Elizabeth Hospital Birmingham
2017-2024
Shanghai Medical College of Fudan University
2018-2024
Zhongshan Hospital of Xiamen University
2024
Jiading District Central Hospital
2024
Shanghai University of Medicine and Health Sciences
2024
Abstract B-cell epitope information is critical to immune therapy and vaccine design. Protein epitopes can be significantly affected by glycosylation, while no methods have considered this till now. Based on previous versions of Spatial Epitope Prediction Antigens (SEPPA), we here present an enhanced tool SEPPA 3.0, enabling glycoprotein antigens. Parameters were updated based the latest largest dataset. Then, additional micro-environmental features glycosylation triangles...
AI alignment aims to make systems behave in line with human intentions and values. As grow more capable, so do risks from misalignment. To provide a comprehensive up-to-date overview of the field, this survey, we delve into core concepts, methodology, practice alignment. First, identify four principles as key objectives alignment: Robustness, Interpretability, Controllability, Ethicality (RICE). Guided by these principles, outline landscape current research decompose them two components:...
Abstract The current SARS-CoV-2 variants strikingly evade all authorized monoclonal antibodies and threaten the efficacy of serum-neutralizing activity elicited by vaccination or prior infection, urging need to develop antivirals against related sarbecoviruses. Here, we identified both potent broadly neutralizing from a five-dose vaccinated donor who exhibited cross-reactive diverse coronaviruses. Through single B-cell sorting sequencing followed tailor-made computational pipeline,...
The emergence of various SARS-CoV-2 variants presents challenges for antibody therapeutics, emphasizing the need more potent and broadly neutralizing antibodies. Here, we employed an unbiased screening approach successfully isolated two antibodies from individuals with only exposure to ancestral SARS-CoV-2. One these antibodies, CYFN1006-1, exhibited robust cross-neutralization against a spectrum variants, including latest KP.2, KP.3 XEC, consistent IC50 values ranging ~1 5 ng/mL. It also...
Spatial Epitope Prediction server for Protein Antigens (SEPPA) has received lots of feedback since being published in 2009. In this improved version, relative ASA preference unit patch and consolidated amino acid index were added as further classification parameters addition to unit-triangle propensity clustering coefficient which previously reported. Then logistic regression model was adopted instead the previous simple additive one. Most importantly, subcellular localization protein...
Flavonoids are the largest class of plant polyphenols, with common structure diphenylpropanes, consisting two aromatic rings linked through three carbons and abundant in both daily diets medicinal plants. Fueled by recognition consuming flavonoids to get better health, researchers became interested deciphering how alter functions human body. Here, systematic studies were performed on 679 flavonoid compounds 481 corresponding targets bioinformatics analysis. Multiple diseases related pathways...
Abstract Identifying the exact epitope positions for a monoclonal antibody (mAb) is of critical importance yet highly challenging to Ab design biomedical research. Based on previous versions SEPPA 3.0, we present SEPPA-mAb above purpose with high accuracy and low false positive rate (FPR), suitable both experimental modelled structures. In practice, appended fingerprints-based patch model considering structural physic-chemical complementarity between possible complementarity-determining...
Background: Colon cancer is one of the most common health threats for humans since its high morbidity and mortality. Detecting potential prognosis risk biomarkers (PRBs) essential improvement therapeutic strategies drug development. Currently, although an integrated prognostic analysis multi-omics colon insufficient, it has been reported to be valuable improving PRBs' detection in other types. Aim: This study aims detect PRBs adenocarcinoma (COAD) samples through genome atlas (TCGA) by...
Major challenges in vaccine development include rapidly selecting or designing immunogens for raising cross-protective immunity against different intra- inter-subtypic pathogens, especially the newly emerging varieties. Here we propose a computational method, Conformational Epitope (CE)-BLAST, calculating antigenic similarity among pathogens with stable and high performance, which is independent of prior binding-assay information, unlike currently available models that heavily rely on...
In this paper, an electrochemical sensing platform based on gold nanoparticle modified carbon nanofiber (CNF@AuNPs) as working electrode is proposed for the high sensitivity and selective determination of metronidazole. Meanwhile, sensor has a large linear response range from 0.1 to 2000 μM, limit detection metronidazole (MNZ) low 0.024 μM. The simple prepare reproducible, it shows good results in MNZ analysis tap water.
The G8 rotavirus genotype has been detected frequently in children many countries and even became the predominant strain sub-Saharan African countries, while there are currently no reports from China. In this study we described genetic characteristics evolutionary relationship between strains Guangzhou China epidemic derived GenBank, 2020-2021.Virus isolation subsequent next-generation sequencing were performed for confirmed G8P[8] specimens. analyzed comparison with sequences obtained...
Retroperitoneal laparoscopic adrenalectomy (RLA) is a minimally invasive technique known for its benefits, including reduced pain and quicker recovery. Despite these advantages, respiratory complications remain notable concern, highlighting the importance of identifying risk factors to enhance perioperative care. This study aimed explore determinants following RLA. A retrospective analysis was conducted on clinical data 569 patients who underwent RLA adrenal lesions in Department Urology at...
Abstract The rapid and consistent mutation of influenza requires frequent evaluation antigenicity variation among newly emerged strains, during which several in-silico methods have been reported to facilitate the assays. In this paper, we designed a structure-based scoring model instead those sequence-based previously published. Protein structural context was adopted derive antigenicity-dominant positions, as well physic-chemical change local micro-environment in correlation with change....
Neoantigen vaccines are one of the most effective immunotherapies for personalized tumour treatment. The current immunogen design neoantigen is usually based on whole-genome sequencing (WGS) and bioinformatics prediction that focuses binding affinity between peptide MHC molecules, ignoring other peptide-presenting related steps. This may result in a gap high accuracy relatively low clinical effectiveness. In this study, we designed an integrated in-silico pipeline, Neo-intline, which started...
ABSTRACT Since the first human infection reported in 2013, H7N9 avian influenza virus (AIV) has been regarded as a serious threat to health. In this study, we sought identify virulence determinant of mammalian hosts. By comparing SH/4664 virus, non-virulent H9N2 and various H7N9-H9N2 hybrid viruses infected mice, pinpointed PB2 primary viral factor accounting for difference between virulence. We further analyzed vivo effects individually mutating residues different from closely related...