A5102726364- Bacterial Genetics and Biotechnology
- Assembly Line Balancing Optimization
- RNA and protein synthesis mechanisms
- Scheduling and Optimization Algorithms
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Manufacturing Process and Optimization
- Protein Structure and Dynamics
- Streptococcal Infections and Treatments
- Electron Spin Resonance Studies
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Bacterial Infections and Vaccines
- Signaling Pathways in Disease
- Advanced Manufacturing and Logistics Optimization
- Trace Elements in Health
- Galectins and Cancer Biology
- Advanced NMR Techniques and Applications
- Lipid Membrane Structure and Behavior
- Bacterial biofilms and quorum sensing
- Amino Acid Enzymes and Metabolism
- Coordination Chemistry and Organometallics
- Hemoglobin structure and function
- Cardiac electrophysiology and arrhythmias
- Amoebic Infections and Treatments
Inserm
2013-2022
Center for Infection and Immunity of Lille
2013-2022
Interactions, Corpus, Apprentissages, Représentations
2022
National Institute of Diabetes and Digestive and Kidney Diseases
2017-2021
National Institutes of Health
2017-2021
Institut Pasteur de Lille
2013-2021
Centre National de la Recherche Scientifique
2013-2021
Nantes Université
2019
École Supérieure de Commerce de Rennes
2019
Université de Lille
2013-2015
FhaC is an integral outer membrane protein of the whooping cough agent Bordetella pertussis that mediates transport to cell surface a major virulence factor, filamentous haemagglutinin adhesin FHA. The FHA/FhaC pair prototypic TpsA/TpsB system widespread 'Two-Partner Secretion' pathway, dedicated long extracellular proteins in various pathogenic and environmental Gram-negative bacteria. belongs ubiquitous Omp85 superfamily transporters. X-ray structure shows transmembrane β-barrel channel...
Bacterial contact-dependent growth inhibition (CDI) systems use a type Vb secretion mechanism to export large CdiA toxins across the outer membrane by dedicated transporters called CdiB. Here, we report first crystal structures of two CdiB from Acinetobacter baumannii and Escherichia coli . adopt TpsB fold, containing 16-stranded transmembrane β-barrel connected periplasmic domains. The lumen pore is occluded an N-terminal α-helix conserved extracellular loop 6; these elements different...
Omp85 transporters mediate protein insertion into, or translocation across, membranes. They have a conserved architecture, with POTRA domains that interact substrate proteins, 16-stranded transmembrane β barrel, and an extracellular loop, L6, folded back in the barrel pore. Here using electrophysiology, vivo biochemical approaches electron paramagnetic resonance, we show L6 loop of transporter FhaC changes conformation modulates channel opening. Those conformational involve breaking...
In this study, we develop a branch and bound solution algorithm to solve the workload smoothing problem. Our incorporates new formulas for dynamically computing lower on optimal value of objective function determining earliest workstations tasks. It also uses fast heuristic good initial upper bound. A comprehensive experimental analysis is conducted in study. The demonstrates outstanding performance its efficiency solving medium-sized problems.
The Two-Partner secretion pathway mediates protein transport across the outer membrane of Gram-negative bacteria. TpsB transporters belong to Omp85 superfamily, whose members catalyze insertion into, or translocation membranes without external energy sources. They are composed a transmembrane β barrel preceded by two periplasmic POTRA domains that bind incoming substrate. Here we used an integrative approach combining in vivo assays, mass spectrometry, nuclear magnetic resonance and electron...
Workload smoothing techniques have been used to establish a balance among stations in assembly lines by assigning the tasks evenly stations. Smoothing facilitates planning workforce and optimizing resource allocation problems. Considering these advantages, we address simple line this research develop problem specific heuristic algorithm solve large instances effectively. We test effectiveness of with experimental study.
Abstract The Two-Partner secretion pathway mediates protein transport across the outer membrane of Gram-negative bacteria. TpsB transporters belong to Omp85 superfamily, whose members catalyze insertion into, or translocation membranes without external energy sources. They are composed a transmembrane β barrel preceded by two periplasmic POTRA domains that bind incoming substrate. Here we used an integrative approach combining in vivo assays, mass spectrometry, nuclear magnetic resonance and...