A5025366120- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Cervical Cancer and HPV Research
- Urinary and Genital Oncology Studies
- Urinary Tract Infections Management
- Genital Health and Disease
- Bacterial Infections and Vaccines
- Infectious Disease Case Reports and Treatments
- Polyomavirus and related diseases
- Amoebic Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Full-Duplex Wireless Communications
- Herpesvirus Infections and Treatments
- Bacillus and Francisella bacterial research
- Mosquito-borne diseases and control
- Parasitic Infections and Diagnostics
- Adolescent Sexual and Reproductive Health
- Indigenous Health and Education
- Clostridium difficile and Clostridium perfringens research
- Poxvirus research and outbreaks
- HIV/AIDS Research and Interventions
- Parvovirus B19 Infection Studies
National Institute of Health Dr. Ricardo Jorge
2010-2025
Instituto Nacional de Saúde
2023
Laboratoire National de Référence
2022
Background Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis ompA -genotypes L1–L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution the LGV -subvariants in Portugal between 2007 2023, order to better understand dissemination diversification landscape strains. Methods The collection Portuguese National Reference Laboratory includes 1188 -genotyped samples 2023. In-depth analysis...
Chlamydia trachomatis is the most prevalent sexually transmitted bacterium worldwide and causative agent of trachoma. Its strains are classified according to their ompA genotypes, which strongly linked differential tissue tropism disease outcomes [ocular disease, urogenital lymphogranuloma venereum (LGV)]. While genome-based species phylogenetic tree presents four main clades correlating with tropism/prevalence, namely ocular, LGV, T1 (more genotypes) T2 (less genotypes), inter-clade...
Objectives Chlamydia trachomatis is classified into 15 major genotypes, A to L3, based on the diversity of ompA gene. Here, we evaluated and characterised distribution -genotypes over 32 years (1990–2021) in Portugal. Methods The collection Portuguese National Reference Laboratory for Sexually Transmitted Infections includes 5824 C . -positive samples that were successfully -genotyped between 1990 2021. An in-depth analysis across years, as well by biological sex, age anatomical site...
R. Lopes da Silva, I. Ferreira, G. Teixeira, D. Cordeiro, M. Mafra, Costa, J.M. Bravo Marques, Abecasis. BK virus encephalitis with thrombotic microangiopathy in an allogeneic hematopoietic stem cell transplant recipient. Transpl Infect Dis 2011: 13: 161–167. All rights reserved Abstract: (BKV) infection occurs most often immunocompromised hosts, the setting of renal or bone marrow transplantation. Hemorrhagic cystitis is commonest manifestation but recent years infections other organ...
To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among Chlamydia trachomatis-positive samples from the collection Portuguese National Reference Laboratory for Sexually Transmitted Infections (STIs), explore a multiplex PCR approach coupled with NGS provide complementary information regarding strain's genomic backbone. A total 502 C. samples, mostly anorectal exudates, were subjected sequencing five targets, including loci potentially...
Previously, we identified a Chlamydia trachomatis lymphogranuloma venereum (LGV) recombinant strain possessing non-LGV ompA genotype. Here, culture-independent genome sequencing confirms its circulation in Europe, Middle East, and North America, unveils emergence of antibiotic resistance. Broad surveillance is needed.
Background Men who have sex with men (MSM) are at risk for sexually transmitted infections (STIs), but more data on extragenital carriage needed. Aim We assessed the genital and prevalence of bacterial other STIs in MSM a Lisbon sexual health clinic. Methods screened oral, anal, urine samples visiting GAT-CheckpointLX clinic June 2017-December 2021 Chlamydia trachomatis (including lymphogranuloma venereum, LGV), Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, hominis,...
<title>Abstract</title> <bold>Background</bold> Lymphogranuloma venereum is a sexually transmitted infection caused by <italic>Chlamydia trachomatis ompA</italic>-genotypes L1-L3, with increasing numbers of detected cases across Europe. Here, we analysed diversity and temporal distribution the LGV <italic>ompA</italic>-subvariants in Portugal between 2007 2023, order to better understand dissemination diversification landscape strains.<bold>Methods</bold> The collection Portuguese National...
<title>Abstract</title> <bold>Objectives: </bold>To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among <italic>C. trachomatis</italic>-positive samples from the collection Portuguese National Reference Laboratory for STI explore application a multiplex PCR approach coupled with Next-Generation Sequencing (NGS) provide complementary information regarding strain’s genomic backbone. <bold>Methods: </bold>502 were subjected sequencing five...
Mpox is a zoonotic disease caused by the Monkeypox virus (MPXV), and since May 2022, tens of thousands cases have been reported in non-endemic countries. We aimed to evaluate suitability different sample types for mpox diagnostic assess temporal dynamics viral load. evaluated 1914 samples from 953 laboratory-confirmed cases. The positivity rate was higher lesion (91.3%) rectal swabs (86.1%) when compared with oropharyngeal (69.5%) urines (41.2%), indicating loads former. Supporting this,...
Abstract Chlamydia trachomatis is the most prevalent sexually transmitted bacteria worldwide and causative agent of blinding trachoma. Strains are classified based on ompA genotypes, which strongly linked with differential tissue tropism disease outcomes. A lymphogranuloma venereum (LGV) epidemics, characterized by ulcerative proctitis, has emerged in last two decades (mainly L2b genotype), raising high concern especially due to its circulation among men who have sex (MSM). Here, we report...
Abstract Between 2001 and 2020, 54 LGV cases were diagnosed in a sexually transmitted disease clinic Lisbon, most men who have sex with (87%), HIV negative (63%), from the anorectal mucosa (72.2%). Cases among heterosexuals also identified (13%). Surveillance programs irrespective of sexual orientation status are needed to avoid morbidity associated LGV.
Background/Objectives: To determine the prevalence of urogenital chlamydia trachomatis (CT), neisseria gonorrhoeae (NG), trichomonas vaginalis (TV), and mycoplasma genitalium (MG) among attendees an open freely available sexually transmitted infections (STI) dedicated clinic in Lisbon, at Centro de Saúde da Lapa, during 1-year.Methods: Molecular testing for CT, NG, MG, TV was performed on 1,062 specimens (one specimen per person).A descriptive, crosssectional, observational study conducted...