A5014508044- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Cell Image Analysis Techniques
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- Nuclear Receptors and Signaling
- RNA Research and Splicing
- Congenital heart defects research
- Neuroinflammation and Neurodegeneration Mechanisms
- Apelin-related biomedical research
- Microfluidic and Bio-sensing Technologies
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Tryptophan and brain disorders
- Health, Environment, Cognitive Aging
- Signaling Pathways in Disease
- Axon Guidance and Neuronal Signaling
- Modular Robots and Swarm Intelligence
- Genomics and Chromatin Dynamics
- Biotin and Related Studies
- Genetics, Aging, and Longevity in Model Organisms
Stanford University
2017-2024
Organogenesis (United States)
2020-2024
Emory University
2022-2024
Neurosciences Institute
2021-2023
Neuroscience Institute
2023
Stony Brook University
2015
Abstract Self-organizing neural organoids represent a promising in vitro platform with which to model human development and disease 1–5 . However, lack the connectivity that exists vivo, limits maturation makes integration other circuits control behaviour impossible. Here we show stem cell-derived cortical transplanted into somatosensory cortex of newborn athymic rats develop mature cell types integrate sensory motivation-related circuits. MRI reveals post-transplantation organoid growth...
The structural and functional complexity of multicellular biological systems, such as the brain, are beyond reach human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials structures if treated anatomically defined compartments for specific chemistry, harnessing biology complex structures. By integrating engineered-enzyme targeting polymer we genetically instructed neurons guide chemical synthesis electrically (conductive insulating)...
Abstract Timothy syndrome (TS) is a severe, multisystem disorder characterized by autism, epilepsy, long-QT and other neuropsychiatric conditions 1 . TS type (TS1) caused gain-of-function variant in the alternatively spliced developmentally enriched CACNA1C exon 8A, as opposed to its counterpart 8. We previously uncovered several phenotypes neurons derived from patients with TS1, including delayed channel inactivation, prolonged depolarization-induced calcium rise, impaired interneuron...
Significance Cell content analysis has rapidly become one of the most important new tools for measuring cell phenotype and behavior. However, central limitation current sampling technologies is they are destructive must lyse cells to measure contents. This destruction prevents knowledge prior or future states cell, which particularly dynamic processes, such as development differentiation. Here, we show a nondestructive longitudinal platform that can sample repeatedly accurately from same...
Abstract Abnormalities in crosstalk between the thalamus and cerebral cortex are thought to lead severe neuropsychiatric disorders, such as epilepsy psychotic disorders. Pathogenic variants CACNA1G gene, which encodes α1G subunit of thalamus-enriched T-type voltage-gated calcium channel CaV3.1, associated with absence seizures, intellectual disability, schizophrenia, but cellular circuit level consequences these genetic humans remain unknown. Here, we developed an vitro human assembloid...
Neuron–glial antigen 2-positive (NG2 + ) glial cells are the most proliferative glia type in adult CNS, and their tile-like arrangement gray matter is under tight regulation. However, little known about cues that govern this unique distribution. To end, using a NG2 cell ablation model mice, we examined repopulation dynamics of mature aged mice matter. We found some resident escaped depletion rapidly enter cycle to repopulate cortex with altered spatial reveal netrin-1 signaling involved...
Assembloids generated from human pluripotent stem cells are self-organizing, multicellular in vitro models that recapitulate aspects of cell-cell interactions and circuit assembly during neural development. Here, we present protocols to functionally monitor, forebrain assembloids, the migration GABAergic interneurons ventral dorsal activity early cortical networks. Specifically, describe high-resolution imaging analysis neuronal as well calcium network assembloids. For complete details on...
SUMMARY Defects in interneuron migration during forebrain development can disrupt the assembly of cortical circuits and have been associated with neuropsychiatric disease. The molecular cellular bases such deficits particularly difficult to study humans due limited access functional tissue from patients. We previously developed a human assembloid model Timothy Syndrome (TS), caused by gain-of-function mutation CACNA1C which encodes L-type calcium channel (LTCC) Ca v 1.2. By functionally...
SUMMARY Extremely preterm born individuals at < 28 postconceptional weeks (PCW) are high risk for encephalopathy of prematurity and life-long neuropsychiatric conditions. Clinical studies animal models brain injury suggest that is strongly associated with exposure to hypoxia and/or inflammation in the perinatal period. Histologic examination postmortem tissue from children demonstrates decreased numbers cortical GABAergic interneurons cerebral cortex. However, cellular molecular...
Abstract Background The locus coeruleus (LC), is the first brain region to develop hyperphosphorylated tau (ptau) inclusions in Alzheimer’s disease (AD) and undergoes catastrophic degeneration later stages of disease. Importantly, LC main noradrenergic nucleus source NE forebrain, dysregulation neurotransmitter norepinephrine (NE) associated with AD symptoms, as its release forebrain regulates attention, arousal, stress response, learning memory. Moreover, may transmit pathogenic via...