A5023995775- Sulfur Compounds in Biology
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- Amino Acid Enzymes and Metabolism
- Cancer Cells and Metastasis
- Herpesvirus Infections and Treatments
- Clinical Laboratory Practices and Quality Control
- Folate and B Vitamins Research
- CAR-T cell therapy research
- HIV Research and Treatment
- Fish Ecology and Management Studies
- Chemokine receptors and signaling
- Pancreatic function and diabetes
- Marine and fisheries research
- Diet and metabolism studies
- HIV/AIDS Research and Interventions
- Radiomics and Machine Learning in Medical Imaging
- Metabolomics and Mass Spectrometry Studies
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- Mesenchymal stem cell research
- S100 Proteins and Annexins
- Animal Genetics and Reproduction
- Coral and Marine Ecosystems Studies
- Cell Adhesion Molecules Research
SUNY Upstate Medical University
2016-2025
The University of Texas Medical Branch at Galveston
2015-2022
Galveston College
2022
East Sussex County Council
2020
Medical College of Wisconsin
2013
University of Louisville
2007-2010
Baxter (United States)
2008
Wesleyan University
2008
Griffith University
2007
NOAA National Marine Fisheries Service
2004
Abstract The trans-sulfuration enzyme cystathionine-β-synthase (CBS) and its product hydrogen sulfide (H2S) are aberrantly upregulated in colorectal cancers, where they contribute to tumor growth progression by both autocrine paracrine mechanisms. However, it is unknown whether the CBS/H2S axis plays a role carcinogenesis. Here, we report upregulation of CBS human biopsies precancerous adenomatous polyps show that forced an adenoma-like colonic epithelial cell line sufficient induce...
We have shown previously that Akt exists in a signal complex with p38 MAPK, MAPK-activated protein kinase-2 (MK2), and heat shock 27 (Hsp27) MK2 phosphorylates on Ser-473. Additionally, dissociation of Hsp27 from Akt, prior to activation, induced polymorphonuclear leukocyte (PMN) apoptosis. However, the role regulating activation was not examined. This study tested hypothesis regulates promotes cell survival by scaffolding complex. Here we show loss Akt/Hsp27 interaction anti-Hsp27 antibody...
IL-6 is a pleiotropic cytokine increased in CRC and known to directly promote tumor growth. Colonic myofibroblasts/fibroblasts (CMFs or stromal cells) are CD90(+) innate immune cells representing up 30% of normal colonic mucosal lamina propria cells. They expanded stroma, where they also as cancer associated fibroblasts (CAFs). Cells mesenchymal origin, such myofibroblasts/fibroblasts, secrete IL-6; however, their contribution the increase tumor-promoting inflammation not well defined. Using...
Colon cancer cells contain high levels of cystathionine-beta-synthase (CBS). Its product, hydrogen sulfide (H2S) promotes the growth and proliferation colorectal tumor cells. In order to improve antitumor efficacy prototypical CBS inhibitor aminooxyacetic acid (AOAA), we have designed synthesized YD0171, a methyl ester derivative AOAA. The antiproliferative effect YD0171 exceeded potency AOAA in HCT116 human colon esterase paraoxon prevented cellular inhibition activity by YD0171. suppressed...
Traumatic brain injury (TBI) is a major cause of death and disability worldwide, with mild TBI (mTBI) accounting for 85% cases. mTBI also implicated in serious long-term sequelae including second impact syndrome chronic traumatic encephalopathy. often goes undiagnosed due to delayed symptom onset limited sensitivity conventional assessment measures compared severe TBI. Current efforts seek identify accurate reliable non-invasive biomarkers associated functional relevant outcomes. Here we...
Herpes simplex viruses (HSV) type 1 are the basis of a number anticancer strategies that have proven efficacious in animal models. They natural human pathogens and majority adults anti-HSV immunity. The current study examined effect preexisting immunity on response to herpes-based oncolytic viral treatment hepatic metastatic cancer murine model designed simulate clinical approach likely be utilized for nonneurological tumors. Specifically, effects NV1020 or G207, two multimutated HSV-1...
Plasma from two rhesus macaques (Macaca mulatta) experimentally infected with the simian immunodeficiency virus (SIV; isolate SIVmac251) enhanced SIVmac infection of a human CD4+ lymphoblastoid cell line, MT-2. Prechallenge plasma samples these animals and serum SIV-negative did not enhance infection. Compared controls, enhancement was characterized by rapid appearance syncytium formation (3 to 4 days sooner), reverse transcriptase release (10-fold increase), cytopathic effect (60% killing)....
Most prostate cancer (PCa)-related deaths are due to metastasis, which is mediated in part by chemokine receptor and corresponding ligand interaction. We have previously shown that PCa tissue cell lines express high levels of the CXCR5, than compared their normal counterparts, interaction CXCR5 with its specific (CXCL13) promoted invasion, migration, differential matrix metalloproteinase (MMP) expression. This study dissects some molecular mechanisms following CXCL13-CXCR5 mediate migration...
Objective To assess the strategy of combining oncolytic herpes simplex virus (HSV) therapy with immunomodulatory as treatment for experimental colon cancer. The HSV recombinant NV1023 and interleukin 12 (IL-12)-secreting NV1042 were evaluated in vitro vivo respect to antitumor efficacy. Summary Background Data Genetically engineered, replication-conditional, attenuated HSVs have shown activity against a wide variety solid malignancies. Other strategies treating cancer involved...
To evaluate mortality of landlocked Atlantic salmon (Salmo salar) caught on flies and worms in a typical river nursery area, study was carried out at the East Outlet Moosehead Lake, Maine, June 1975-1977. Of 177 fish 3 yr, 22% died after hooking. All 74 control fishway trap survived. Mortality worm-hooked (35%) significantly greater than that hooked (4%). Higher attributed largely to anatomical site Nearly 37% worm-caught were esophagus, but most fly-caught either jaws or mouth. bled hooking...
Patient-derived xenografting (PDX) of human colorectal cancer (CRC) is the preferred experimental model to study tumor response therapeutic agents. Gradually, stromal cells are replaced by mouse cells; however, exact timing replacement with murine in CRC xenograft has not been fully elucidated. We hypothesize that orthologous transcripts functionally substitutes for loss due genes.Human were implanted athymic nude mice replicates and followed-up over time. Using next-generation sequencing,...
Little is known about the presence and function of tissue-resident mesenchymal stem cells [MtSCs] within gastrointestinal mucosa in health inflammatory bowel disease [IBD]. The contribution MtSCs to generation fibroblasts during IBD also poorly understood. We hypothesized that IBD-MtSCs are impaired contribute pathological myofibroblasts IBD. In a cohort clinically endoscopically active patients normal controls, we used quantitative RT-PCR cell differentiation assays, as well confocal...
Behavior-analytic treatments successfully reduce individuals’ destructive behavior (e.g., self-injurious behavior, aggression, property destruction, disruption); however, there is limited research evaluating these treatments’ effects on physiological stress responses, even though can have significant negative impacts quality of life. Research from other fields has demonstrated that salivary cortisol concentration a valid biomarker for stress, so researchers could potentially use this to...
American lobsters (Homarus americanus) are native to the east coast of North and Canada, but have been imported live into Europe for several decades resulting in their escape wild. potential a significant impact on European lobster gammarus) fisheries Europe, status is not well understood, especially Great Britain (GB) where reports sporadic. Reports were collated from across GB being caught coastal waters. Between 1988 2011, 26 individuals positively identified using standard taxonomic...
Ulcerative colitis (UC) is characterized by widespread relapsing inflammation of the colonic mucosa. Colitis-associated cancer (CAC) one most serious complications a prolonged history UC. Hydrogen sulfide (H2S) has emerged as an important physiological mediator gastrointestinal homeostasis, limiting mucosal and promoting tissue healing in response to injury. Inhibition cystathionine-γ-lyase (CSE)-dependent H2S production animal models UC been shown exacerbate delay repair. It unknown whether...
Simian immunodeficiency virus (SIV) infection of macaques is a useful and relevant model for evaluating candidate human (HIV) vaccines. One important feature this that SIV vaccines can be evaluated their ability to prevent as well or delay the onset AIDS. In present study, group was vaccinated with whole inactivated challenged peripheral blood mononuclear cells from an SIV-infected macaque. This challenge represented rigorous realistic test immunization protocol. All became infected after...
Background Published literature has demonstrated commercially available premix vancomycin (5 mg/mL) and piperacillin-tazobactam (67.5 as physically compatible via simulated Y-site methodology. Compatibility actual infusion yet to be established. Objective To assess compare the compatibility of concentrations evaluation. Methods Vancomycin were tested using methodologies. Simulated was performed previously published methods visual inspection, turbidity evaluation, pH Evaluation occurred...
Fibroblasts/myofibroblasts (MFs) have been gaining increasing attention for their role in pathogenesis and contributions to both wound healing promotion of the tumor microenvironment. While there are currently many techniques isolation MFs from gastrointestinal (GI) tissues, this protocol introduces a novel element these stromal cells frozen tissue. Freezing GI tissue specimens not only allows researcher acquire samples worldwide collaborators, biobanks, commercial vendors, it also permits...